Experiment: R2A with Cesium chloride 1.75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2197 and Atu2198 are separated by 147 nucleotides Atu2198 and Atu2199 are separated by 155 nucleotides Atu2199 and Atu8026 are separated by 58 nucleotides Atu8026 and Atu2200 are separated by 267 nucleotides Atu2200 and Atu2201 are separated by 84 nucleotides
Atu2197: Atu2197 - hypothetical protein, at 2,171,033 to 2,171,959
Atu2197
Atu2198: Atu2198 - hypothetical protein, at 2,172,107 to 2,172,418
Atu2198
Atu2199: Atu2199 - permease, at 2,172,574 to 2,173,476
Atu2199
Atu8026: Atu8026 - hypothetical protein, at 2,173,535 to 2,173,654
Atu8026
Atu2200: Atu2200 - cold shock protein, at 2,173,922 to 2,174,137
Atu2200
Atu2201: Atu2201 - hydrolase, at 2,174,222 to 2,174,866
Atu2201
Position (kb)
2172
2173
2174 Strain fitness (log2 ratio)
-2
-1
0
1 at 2171.632 kb on + strand, within Atu2197 at 2171.656 kb on - strand, within Atu2197 at 2171.678 kb on + strand, within Atu2197 at 2171.751 kb on + strand, within Atu2197 at 2171.767 kb on + strand, within Atu2197 at 2171.769 kb on + strand, within Atu2197 at 2171.775 kb on - strand, within Atu2197 at 2171.929 kb on + strand at 2171.937 kb on - strand at 2171.938 kb on - strand at 2171.938 kb on - strand at 2171.945 kb on - strand at 2172.069 kb on + strand at 2172.077 kb on - strand at 2172.077 kb on - strand at 2172.138 kb on + strand at 2172.227 kb on - strand, within Atu2198 at 2172.256 kb on - strand, within Atu2198 at 2172.259 kb on - strand, within Atu2198 at 2172.265 kb on - strand, within Atu2198 at 2172.265 kb on - strand, within Atu2198 at 2172.268 kb on - strand, within Atu2198 at 2172.275 kb on - strand, within Atu2198 at 2172.306 kb on + strand, within Atu2198 at 2172.319 kb on - strand, within Atu2198 at 2172.349 kb on - strand, within Atu2198 at 2172.379 kb on - strand, within Atu2198 at 2172.402 kb on - strand at 2172.571 kb on + strand at 2172.591 kb on - strand at 2172.665 kb on - strand, within Atu2199 at 2172.675 kb on - strand, within Atu2199 at 2172.723 kb on - strand, within Atu2199 at 2172.723 kb on - strand, within Atu2199 at 2172.723 kb on - strand, within Atu2199 at 2172.782 kb on + strand, within Atu2199 at 2172.782 kb on + strand, within Atu2199 at 2172.790 kb on - strand, within Atu2199 at 2172.805 kb on - strand, within Atu2199 at 2172.867 kb on - strand, within Atu2199 at 2172.933 kb on + strand, within Atu2199 at 2172.943 kb on + strand, within Atu2199 at 2172.951 kb on - strand, within Atu2199 at 2172.951 kb on - strand, within Atu2199 at 2172.977 kb on + strand, within Atu2199 at 2173.035 kb on + strand, within Atu2199 at 2173.047 kb on + strand, within Atu2199 at 2173.047 kb on + strand, within Atu2199 at 2173.123 kb on - strand, within Atu2199 at 2173.136 kb on + strand, within Atu2199 at 2173.163 kb on - strand, within Atu2199 at 2173.167 kb on + strand, within Atu2199 at 2173.272 kb on + strand, within Atu2199 at 2173.280 kb on - strand, within Atu2199 at 2173.280 kb on - strand, within Atu2199 at 2173.285 kb on + strand, within Atu2199 at 2173.449 kb on + strand at 2173.457 kb on - strand at 2173.871 kb on + strand at 2173.871 kb on + strand at 2173.871 kb on - strand at 2173.889 kb on - strand at 2173.911 kb on + strand at 2173.925 kb on + strand at 2174.029 kb on + strand, within Atu2200 at 2174.039 kb on - strand, within Atu2200 at 2174.049 kb on - strand, within Atu2200 at 2174.102 kb on + strand, within Atu2200 at 2174.146 kb on - strand at 2174.210 kb on + strand at 2174.218 kb on - strand at 2174.267 kb on - strand at 2174.286 kb on - strand at 2174.291 kb on + strand, within Atu2201
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cesium chloride 1.75 mM remove 2,171,632 + Atu2197 0.65 -2.0 2,171,656 - Atu2197 0.67 -0.1 2,171,678 + Atu2197 0.70 +0.4 2,171,751 + Atu2197 0.77 -0.7 2,171,767 + Atu2197 0.79 +0.7 2,171,769 + Atu2197 0.79 -0.2 2,171,775 - Atu2197 0.80 +0.1 2,171,929 + -0.2 2,171,937 - +0.0 2,171,938 - -0.4 2,171,938 - +0.4 2,171,945 - -0.3 2,172,069 + +0.1 2,172,077 - -0.2 2,172,077 - +0.3 2,172,138 + -0.1 2,172,227 - Atu2198 0.38 -0.7 2,172,256 - Atu2198 0.48 -0.1 2,172,259 - Atu2198 0.49 -0.3 2,172,265 - Atu2198 0.51 +0.1 2,172,265 - Atu2198 0.51 +1.7 2,172,268 - Atu2198 0.52 +0.2 2,172,275 - Atu2198 0.54 +0.0 2,172,306 + Atu2198 0.64 +0.2 2,172,319 - Atu2198 0.68 +0.1 2,172,349 - Atu2198 0.78 -0.1 2,172,379 - Atu2198 0.87 -0.4 2,172,402 - -0.0 2,172,571 + -1.0 2,172,591 - -1.0 2,172,665 - Atu2199 0.10 -1.4 2,172,675 - Atu2199 0.11 -0.2 2,172,723 - Atu2199 0.17 -1.1 2,172,723 - Atu2199 0.17 -0.5 2,172,723 - Atu2199 0.17 +0.3 2,172,782 + Atu2199 0.23 -0.6 2,172,782 + Atu2199 0.23 -0.8 2,172,790 - Atu2199 0.24 -0.7 2,172,805 - Atu2199 0.26 -0.7 2,172,867 - Atu2199 0.32 -2.4 2,172,933 + Atu2199 0.40 -1.1 2,172,943 + Atu2199 0.41 -0.8 2,172,951 - Atu2199 0.42 -0.9 2,172,951 - Atu2199 0.42 -0.5 2,172,977 + Atu2199 0.45 -0.1 2,173,035 + Atu2199 0.51 +0.2 2,173,047 + Atu2199 0.52 -0.9 2,173,047 + Atu2199 0.52 -0.9 2,173,123 - Atu2199 0.61 -0.8 2,173,136 + Atu2199 0.62 -1.1 2,173,163 - Atu2199 0.65 -0.7 2,173,167 + Atu2199 0.66 -0.8 2,173,272 + Atu2199 0.77 -0.0 2,173,280 - Atu2199 0.78 -0.0 2,173,280 - Atu2199 0.78 -0.6 2,173,285 + Atu2199 0.79 -0.8 2,173,449 + -0.3 2,173,457 - -1.1 2,173,871 + -0.0 2,173,871 + -0.1 2,173,871 - +0.1 2,173,889 - +1.0 2,173,911 + +0.0 2,173,925 + +0.1 2,174,029 + Atu2200 0.50 -0.2 2,174,039 - Atu2200 0.54 +0.5 2,174,049 - Atu2200 0.59 +0.5 2,174,102 + Atu2200 0.83 +0.3 2,174,146 - -0.3 2,174,210 + -0.2 2,174,218 - +0.4 2,174,267 - +0.1 2,174,286 - -0.6 2,174,291 + Atu2201 0.11 +1.7
Or see this region's nucleotide sequence