Experiment: R2A with Chlorate 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5123 and Atu5124 overlap by 1 nucleotides Atu5124 and Atu5125 are separated by 114 nucleotides
Atu5123: Atu5123 - acetolactate synthase catalytic subunit, at 129,201 to 130,916
Atu5123
Atu5124: Atu5124 - glutamate-1-semialdehyde aminotransferase, at 130,916 to 132,250
Atu5124
Atu5125: Atu5125 - GntR family transcriptional regulator, at 132,365 to 133,054
Atu5125
Position (kb)
130
131
132
133 Strain fitness (log2 ratio)
-1
0
1 at 129.993 kb on - strand, within Atu5123 at 130.020 kb on - strand, within Atu5123 at 130.020 kb on - strand, within Atu5123 at 130.044 kb on - strand, within Atu5123 at 130.126 kb on - strand, within Atu5123 at 130.174 kb on + strand, within Atu5123 at 130.182 kb on - strand, within Atu5123 at 130.215 kb on - strand, within Atu5123 at 130.247 kb on - strand, within Atu5123 at 130.249 kb on - strand, within Atu5123 at 130.351 kb on - strand, within Atu5123 at 130.405 kb on - strand, within Atu5123 at 130.545 kb on - strand, within Atu5123 at 130.598 kb on - strand, within Atu5123 at 130.634 kb on + strand, within Atu5123 at 130.647 kb on - strand, within Atu5123 at 130.656 kb on - strand, within Atu5123 at 130.687 kb on - strand, within Atu5123 at 130.689 kb on + strand, within Atu5123 at 130.697 kb on - strand, within Atu5123 at 130.697 kb on - strand, within Atu5123 at 130.735 kb on + strand, within Atu5123 at 130.789 kb on - strand at 130.789 kb on - strand at 130.789 kb on - strand at 130.986 kb on + strand at 131.072 kb on - strand, within Atu5124 at 131.210 kb on + strand, within Atu5124 at 131.210 kb on + strand, within Atu5124 at 131.218 kb on - strand, within Atu5124 at 131.218 kb on - strand, within Atu5124 at 131.275 kb on - strand, within Atu5124 at 131.325 kb on + strand, within Atu5124 at 131.325 kb on + strand, within Atu5124 at 131.383 kb on + strand, within Atu5124 at 131.389 kb on - strand, within Atu5124 at 131.429 kb on - strand, within Atu5124 at 131.449 kb on - strand, within Atu5124 at 131.506 kb on - strand, within Atu5124 at 131.506 kb on - strand, within Atu5124 at 131.506 kb on - strand, within Atu5124 at 131.549 kb on + strand, within Atu5124 at 131.567 kb on + strand, within Atu5124 at 131.569 kb on + strand, within Atu5124 at 131.577 kb on - strand, within Atu5124 at 131.579 kb on - strand, within Atu5124 at 131.590 kb on + strand, within Atu5124 at 131.590 kb on - strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.881 kb on + strand, within Atu5124 at 131.894 kb on + strand, within Atu5124 at 131.974 kb on - strand, within Atu5124 at 131.984 kb on - strand, within Atu5124 at 131.984 kb on - strand, within Atu5124 at 132.011 kb on - strand, within Atu5124 at 132.121 kb on + strand at 132.144 kb on - strand at 132.148 kb on + strand at 132.525 kb on + strand, within Atu5125 at 132.533 kb on - strand, within Atu5125 at 132.533 kb on - strand, within Atu5125 at 132.541 kb on + strand, within Atu5125 at 132.549 kb on - strand, within Atu5125 at 132.565 kb on + strand, within Atu5125 at 132.786 kb on - strand, within Atu5125 at 132.834 kb on - strand, within Atu5125 at 132.877 kb on - strand, within Atu5125 at 132.892 kb on - strand, within Atu5125 at 132.899 kb on + strand, within Atu5125 at 132.920 kb on + strand, within Atu5125 at 132.966 kb on + strand, within Atu5125 at 133.009 kb on + strand at 133.036 kb on - strand at 133.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorate 400 mM remove 129,993 - Atu5123 0.46 -0.2 130,020 - Atu5123 0.48 +0.2 130,020 - Atu5123 0.48 -1.0 130,044 - Atu5123 0.49 -0.0 130,126 - Atu5123 0.54 -0.2 130,174 + Atu5123 0.57 +0.1 130,182 - Atu5123 0.57 -0.5 130,215 - Atu5123 0.59 +0.6 130,247 - Atu5123 0.61 +0.0 130,249 - Atu5123 0.61 -0.2 130,351 - Atu5123 0.67 +0.1 130,405 - Atu5123 0.70 +0.8 130,545 - Atu5123 0.78 -0.3 130,598 - Atu5123 0.81 +0.5 130,634 + Atu5123 0.84 -0.0 130,647 - Atu5123 0.84 -0.1 130,656 - Atu5123 0.85 -0.6 130,687 - Atu5123 0.87 +0.2 130,689 + Atu5123 0.87 -0.3 130,697 - Atu5123 0.87 +0.5 130,697 - Atu5123 0.87 +1.2 130,735 + Atu5123 0.89 -0.2 130,789 - -0.1 130,789 - -0.4 130,789 - -0.0 130,986 + +0.4 131,072 - Atu5124 0.12 +0.2 131,210 + Atu5124 0.22 -0.5 131,210 + Atu5124 0.22 +0.4 131,218 - Atu5124 0.23 -0.3 131,218 - Atu5124 0.23 +0.5 131,275 - Atu5124 0.27 -0.3 131,325 + Atu5124 0.31 -0.6 131,325 + Atu5124 0.31 +0.0 131,383 + Atu5124 0.35 +0.3 131,389 - Atu5124 0.35 +1.0 131,429 - Atu5124 0.38 -0.1 131,449 - Atu5124 0.40 +0.1 131,506 - Atu5124 0.44 -0.3 131,506 - Atu5124 0.44 +0.3 131,506 - Atu5124 0.44 -0.1 131,549 + Atu5124 0.47 -0.1 131,567 + Atu5124 0.49 +0.3 131,569 + Atu5124 0.49 +0.3 131,577 - Atu5124 0.50 -0.4 131,579 - Atu5124 0.50 +0.3 131,590 + Atu5124 0.50 -0.4 131,590 - Atu5124 0.50 -0.1 131,777 + Atu5124 0.64 -0.7 131,777 + Atu5124 0.64 -0.1 131,777 + Atu5124 0.64 +0.4 131,777 + Atu5124 0.64 -1.0 131,785 - Atu5124 0.65 -0.0 131,785 - Atu5124 0.65 +0.3 131,785 - Atu5124 0.65 -0.4 131,785 - Atu5124 0.65 +0.2 131,785 - Atu5124 0.65 +1.0 131,799 + Atu5124 0.66 +0.9 131,799 + Atu5124 0.66 -0.4 131,799 + Atu5124 0.66 +0.3 131,799 + Atu5124 0.66 +0.5 131,807 - Atu5124 0.67 +0.5 131,807 - Atu5124 0.67 -0.1 131,807 - Atu5124 0.67 +0.3 131,807 - Atu5124 0.67 -0.1 131,881 + Atu5124 0.72 -0.6 131,894 + Atu5124 0.73 +0.2 131,974 - Atu5124 0.79 -0.2 131,984 - Atu5124 0.80 -0.1 131,984 - Atu5124 0.80 -0.5 132,011 - Atu5124 0.82 -0.1 132,121 + +1.0 132,144 - +0.1 132,148 + -0.2 132,525 + Atu5125 0.23 -0.1 132,533 - Atu5125 0.24 -0.5 132,533 - Atu5125 0.24 -0.7 132,541 + Atu5125 0.26 +0.3 132,549 - Atu5125 0.27 -1.7 132,565 + Atu5125 0.29 -0.4 132,786 - Atu5125 0.61 -1.2 132,834 - Atu5125 0.68 -0.3 132,877 - Atu5125 0.74 -0.0 132,892 - Atu5125 0.76 +0.7 132,899 + Atu5125 0.77 -0.5 132,920 + Atu5125 0.80 -0.5 132,966 + Atu5125 0.87 +0.3 133,009 + -1.4 133,036 - -1.2 133,218 - -0.1
Or see this region's nucleotide sequence