Experiment: R2A with Chlorate 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3675 and Atu3676 overlap by 4 nucleotides Atu3676 and Atu3677 overlap by 4 nucleotides Atu3677 and Atu3678 are separated by 14 nucleotides Atu3678 and Atu3679 are separated by 10 nucleotides Atu3679 and Atu3680 overlap by 4 nucleotides
Atu3675: Atu3675 - peptide synthetase, siderophore biosynthesis protein, at 729,769 to 734,331
Atu3675
Atu3676: Atu3676 - siderophore biosynthesis protein, at 734,328 to 735,224
Atu3676
Atu3677: Atu3677 - polyketide synthase, at 735,221 to 736,696
Atu3677
Atu3678: Atu3678 - siderophore biosynthesis protein, at 736,711 to 736,950
Atu3678
Atu3679: Atu3679 - siderophore biosynthesis protein, at 736,961 to 737,479
Atu3679
Atu3680: Atu3680 - siderophore biosynthesis protein, at 737,476 to 738,039
Atu3680
Position (kb) 735
736
737 Strain fitness (log2 ratio) -1
0
1
2 at 734.237 kb on + strand at 734.335 kb on + strand at 734.335 kb on + strand at 734.337 kb on + strand at 734.343 kb on - strand at 734.386 kb on + strand at 734.400 kb on - strand at 734.464 kb on - strand, within Atu3676 at 734.464 kb on - strand, within Atu3676 at 734.518 kb on - strand, within Atu3676 at 734.637 kb on - strand, within Atu3676 at 734.663 kb on - strand, within Atu3676 at 734.663 kb on - strand, within Atu3676 at 734.692 kb on + strand, within Atu3676 at 734.717 kb on - strand, within Atu3676 at 734.797 kb on - strand, within Atu3676 at 734.810 kb on - strand, within Atu3676 at 734.889 kb on + strand, within Atu3676 at 734.906 kb on + strand, within Atu3676 at 734.940 kb on + strand, within Atu3676 at 735.046 kb on - strand, within Atu3676 at 735.068 kb on - strand, within Atu3676 at 735.161 kb on - strand at 735.161 kb on - strand at 735.192 kb on + strand at 735.200 kb on - strand at 735.264 kb on - strand at 735.455 kb on - strand, within Atu3677 at 735.537 kb on + strand, within Atu3677 at 735.563 kb on - strand, within Atu3677 at 735.604 kb on + strand, within Atu3677 at 735.711 kb on - strand, within Atu3677 at 735.752 kb on + strand, within Atu3677 at 735.752 kb on + strand, within Atu3677 at 735.760 kb on - strand, within Atu3677 at 735.817 kb on - strand, within Atu3677 at 735.817 kb on - strand, within Atu3677 at 735.927 kb on - strand, within Atu3677 at 735.960 kb on - strand, within Atu3677 at 735.966 kb on + strand, within Atu3677 at 736.006 kb on - strand, within Atu3677 at 736.006 kb on - strand, within Atu3677 at 736.117 kb on + strand, within Atu3677 at 736.217 kb on + strand, within Atu3677 at 736.346 kb on + strand, within Atu3677 at 736.369 kb on + strand, within Atu3677 at 736.555 kb on - strand at 736.555 kb on - strand at 736.562 kb on + strand at 736.568 kb on - strand at 736.699 kb on + strand at 736.728 kb on + strand at 736.743 kb on + strand, within Atu3678 at 736.750 kb on + strand, within Atu3678 at 736.768 kb on + strand, within Atu3678 at 736.791 kb on + strand, within Atu3678 at 736.797 kb on - strand, within Atu3678 at 736.848 kb on - strand, within Atu3678 at 737.022 kb on + strand, within Atu3679 at 737.024 kb on - strand, within Atu3679 at 737.027 kb on + strand, within Atu3679 at 737.050 kb on + strand, within Atu3679 at 737.149 kb on + strand, within Atu3679 at 737.342 kb on - strand, within Atu3679 at 737.361 kb on - strand, within Atu3679 at 737.467 kb on - strand at 737.469 kb on + strand at 737.475 kb on - strand at 737.557 kb on - strand, within Atu3680 at 737.557 kb on - strand, within Atu3680 at 737.610 kb on + strand, within Atu3680 at 737.648 kb on + strand, within Atu3680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorate 400 mM remove 734,237 + +0.0 734,335 + -0.0 734,335 + -0.3 734,337 + +0.0 734,343 - -0.3 734,386 + -0.3 734,400 - +1.0 734,464 - Atu3676 0.15 -0.0 734,464 - Atu3676 0.15 -0.1 734,518 - Atu3676 0.21 -1.0 734,637 - Atu3676 0.34 -0.0 734,663 - Atu3676 0.37 -0.4 734,663 - Atu3676 0.37 +0.4 734,692 + Atu3676 0.41 -0.2 734,717 - Atu3676 0.43 -0.2 734,797 - Atu3676 0.52 -0.4 734,810 - Atu3676 0.54 +0.1 734,889 + Atu3676 0.63 -1.1 734,906 + Atu3676 0.64 -0.9 734,940 + Atu3676 0.68 -0.4 735,046 - Atu3676 0.80 +0.8 735,068 - Atu3676 0.82 -0.1 735,161 - +0.1 735,161 - -0.8 735,192 + +0.2 735,200 - -0.2 735,264 - -1.1 735,455 - Atu3677 0.16 -0.0 735,537 + Atu3677 0.21 +0.3 735,563 - Atu3677 0.23 -0.0 735,604 + Atu3677 0.26 +0.1 735,711 - Atu3677 0.33 +0.6 735,752 + Atu3677 0.36 -0.6 735,752 + Atu3677 0.36 -1.6 735,760 - Atu3677 0.37 +0.1 735,817 - Atu3677 0.40 -0.1 735,817 - Atu3677 0.40 +1.1 735,927 - Atu3677 0.48 +0.0 735,960 - Atu3677 0.50 -0.5 735,966 + Atu3677 0.50 -0.3 736,006 - Atu3677 0.53 +1.7 736,006 - Atu3677 0.53 -0.7 736,117 + Atu3677 0.61 +0.3 736,217 + Atu3677 0.67 +0.0 736,346 + Atu3677 0.76 +0.3 736,369 + Atu3677 0.78 -0.3 736,555 - -0.3 736,555 - +0.2 736,562 + -0.3 736,568 - +2.3 736,699 + +0.4 736,728 + -0.2 736,743 + Atu3678 0.13 +0.6 736,750 + Atu3678 0.16 -0.4 736,768 + Atu3678 0.24 -1.6 736,791 + Atu3678 0.33 +0.4 736,797 - Atu3678 0.36 -0.6 736,848 - Atu3678 0.57 +0.0 737,022 + Atu3679 0.12 +0.2 737,024 - Atu3679 0.12 +0.4 737,027 + Atu3679 0.13 +0.4 737,050 + Atu3679 0.17 -0.5 737,149 + Atu3679 0.36 +0.3 737,342 - Atu3679 0.73 -0.2 737,361 - Atu3679 0.77 -0.2 737,467 - -0.0 737,469 + -0.2 737,475 - -0.4 737,557 - Atu3680 0.14 +0.2 737,557 - Atu3680 0.14 +0.2 737,610 + Atu3680 0.24 +0.4 737,648 + Atu3680 0.30 -0.5
Or see this region's nucleotide sequence