Experiment: R2A with Chlorate 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3473 and Atu3474 are separated by 44 nucleotides Atu3474 and Atu3475 are separated by 11 nucleotides
Atu3473: Atu3473 - 2-oxoisovalerate dehydrogenase subunit alpha, at 520,202 to 521,440
Atu3473
Atu3474: Atu3474 - acyl-CoA dehydrogenase, at 521,485 to 522,612
Atu3474
Atu3475: Atu3475 - acetyl-CoA C-acetyltransferase, at 522,624 to 523,862
Atu3475
Position (kb) 521
522
523 Strain fitness (log2 ratio) -2
-1
0
1
2 at 520.501 kb on - strand, within Atu3473 at 520.534 kb on + strand, within Atu3473 at 520.542 kb on - strand, within Atu3473 at 520.547 kb on - strand, within Atu3473 at 520.572 kb on - strand, within Atu3473 at 520.583 kb on + strand, within Atu3473 at 520.583 kb on + strand, within Atu3473 at 520.591 kb on - strand, within Atu3473 at 520.591 kb on - strand, within Atu3473 at 520.629 kb on - strand, within Atu3473 at 520.739 kb on - strand, within Atu3473 at 520.931 kb on - strand, within Atu3473 at 520.979 kb on - strand, within Atu3473 at 521.134 kb on - strand, within Atu3473 at 521.226 kb on + strand, within Atu3473 at 521.282 kb on + strand, within Atu3473 at 521.290 kb on - strand, within Atu3473 at 521.300 kb on + strand, within Atu3473 at 521.334 kb on + strand at 521.342 kb on - strand at 521.459 kb on + strand at 521.463 kb on + strand at 521.464 kb on + strand at 521.467 kb on - strand at 521.509 kb on + strand at 521.528 kb on - strand at 521.566 kb on - strand at 521.694 kb on - strand, within Atu3474 at 521.699 kb on - strand, within Atu3474 at 521.746 kb on - strand, within Atu3474 at 521.803 kb on + strand, within Atu3474 at 521.838 kb on - strand, within Atu3474 at 521.848 kb on - strand, within Atu3474 at 521.929 kb on - strand, within Atu3474 at 521.946 kb on + strand, within Atu3474 at 521.954 kb on - strand, within Atu3474 at 522.029 kb on + strand, within Atu3474 at 522.034 kb on - strand, within Atu3474 at 522.146 kb on - strand, within Atu3474 at 522.156 kb on + strand, within Atu3474 at 522.164 kb on - strand, within Atu3474 at 522.174 kb on - strand, within Atu3474 at 522.174 kb on - strand, within Atu3474 at 522.193 kb on - strand, within Atu3474 at 522.203 kb on + strand, within Atu3474 at 522.210 kb on - strand, within Atu3474 at 522.244 kb on - strand, within Atu3474 at 522.622 kb on + strand at 522.630 kb on - strand at 522.710 kb on - strand at 522.942 kb on + strand, within Atu3475 at 522.942 kb on + strand, within Atu3475 at 523.113 kb on - strand, within Atu3475 at 523.113 kb on - strand, within Atu3475 at 523.202 kb on + strand, within Atu3475 at 523.233 kb on + strand, within Atu3475 at 523.236 kb on - strand, within Atu3475 at 523.241 kb on - strand, within Atu3475 at 523.248 kb on + strand, within Atu3475 at 523.290 kb on - strand, within Atu3475 at 523.349 kb on + strand, within Atu3475 at 523.474 kb on + strand, within Atu3475 at 523.486 kb on - strand, within Atu3475 at 523.603 kb on + strand, within Atu3475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorate 400 mM remove 520,501 - Atu3473 0.24 -0.3 520,534 + Atu3473 0.27 -1.3 520,542 - Atu3473 0.27 +0.3 520,547 - Atu3473 0.28 -0.7 520,572 - Atu3473 0.30 +0.1 520,583 + Atu3473 0.31 +0.8 520,583 + Atu3473 0.31 -1.2 520,591 - Atu3473 0.31 -0.4 520,591 - Atu3473 0.31 +1.2 520,629 - Atu3473 0.34 -1.5 520,739 - Atu3473 0.43 -1.0 520,931 - Atu3473 0.59 -0.9 520,979 - Atu3473 0.63 -1.1 521,134 - Atu3473 0.75 -0.2 521,226 + Atu3473 0.83 -0.5 521,282 + Atu3473 0.87 -0.1 521,290 - Atu3473 0.88 -1.2 521,300 + Atu3473 0.89 -0.4 521,334 + -0.3 521,342 - -0.1 521,459 + -0.8 521,463 + -0.3 521,464 + +0.2 521,467 - +0.1 521,509 + +1.1 521,528 - -0.2 521,566 - +0.1 521,694 - Atu3474 0.19 -0.0 521,699 - Atu3474 0.19 -1.9 521,746 - Atu3474 0.23 -0.0 521,803 + Atu3474 0.28 -0.2 521,838 - Atu3474 0.31 -0.5 521,848 - Atu3474 0.32 +1.2 521,929 - Atu3474 0.39 -0.5 521,946 + Atu3474 0.41 -0.7 521,954 - Atu3474 0.42 -0.9 522,029 + Atu3474 0.48 -2.4 522,034 - Atu3474 0.49 -0.4 522,146 - Atu3474 0.59 -0.7 522,156 + Atu3474 0.59 -0.2 522,164 - Atu3474 0.60 -1.1 522,174 - Atu3474 0.61 -0.6 522,174 - Atu3474 0.61 -0.8 522,193 - Atu3474 0.63 -0.5 522,203 + Atu3474 0.64 +0.0 522,210 - Atu3474 0.64 -0.3 522,244 - Atu3474 0.67 -0.6 522,622 + -0.3 522,630 - +0.2 522,710 - +0.1 522,942 + Atu3475 0.26 -0.3 522,942 + Atu3475 0.26 +0.0 523,113 - Atu3475 0.39 +0.3 523,113 - Atu3475 0.39 -0.6 523,202 + Atu3475 0.47 -1.2 523,233 + Atu3475 0.49 +2.0 523,236 - Atu3475 0.49 -0.1 523,241 - Atu3475 0.50 -0.6 523,248 + Atu3475 0.50 -0.9 523,290 - Atu3475 0.54 +0.6 523,349 + Atu3475 0.59 +0.3 523,474 + Atu3475 0.69 -0.9 523,486 - Atu3475 0.70 -0.0 523,603 + Atu3475 0.79 -0.1
Or see this region's nucleotide sequence