Experiment: R2A with nitrite 9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3178 and Atu3179 are separated by 28 nucleotides Atu3179 and Atu3180 overlap by 8 nucleotides
Atu3178: Atu3178 - ABC transporter substrate binding protein, at 188,009 to 189,022
Atu3178
Atu3179: Atu3179 - ABC transporter permease, at 189,051 to 189,917
Atu3179
Atu3180: Atu3180 - ABC transporter permease, at 189,910 to 190,743
Atu3180
Position (kb)
189
190 Strain fitness (log2 ratio)
-2
-1
0
1 at 188.059 kb on + strand at 188.067 kb on - strand at 188.120 kb on + strand, within Atu3178 at 188.133 kb on - strand, within Atu3178 at 188.134 kb on - strand, within Atu3178 at 188.139 kb on - strand, within Atu3178 at 188.207 kb on - strand, within Atu3178 at 188.207 kb on - strand, within Atu3178 at 188.216 kb on - strand, within Atu3178 at 188.495 kb on + strand, within Atu3178 at 188.503 kb on - strand, within Atu3178 at 188.691 kb on + strand, within Atu3178 at 188.699 kb on - strand, within Atu3178 at 188.699 kb on - strand, within Atu3178 at 188.699 kb on - strand, within Atu3178 at 188.777 kb on - strand, within Atu3178 at 188.932 kb on - strand at 188.946 kb on + strand at 188.946 kb on + strand at 188.954 kb on - strand at 189.020 kb on - strand at 189.020 kb on - strand at 189.020 kb on - strand at 189.020 kb on - strand at 189.058 kb on + strand at 189.066 kb on - strand at 189.066 kb on - strand at 189.116 kb on - strand at 189.191 kb on - strand, within Atu3179 at 189.196 kb on - strand, within Atu3179 at 189.240 kb on + strand, within Atu3179 at 189.240 kb on + strand, within Atu3179 at 189.248 kb on - strand, within Atu3179 at 189.259 kb on + strand, within Atu3179 at 189.259 kb on + strand, within Atu3179 at 189.262 kb on - strand, within Atu3179 at 189.267 kb on - strand, within Atu3179 at 189.267 kb on - strand, within Atu3179 at 189.349 kb on - strand, within Atu3179 at 189.398 kb on - strand, within Atu3179 at 189.424 kb on - strand, within Atu3179 at 189.456 kb on - strand, within Atu3179 at 189.484 kb on + strand, within Atu3179 at 189.600 kb on + strand, within Atu3179 at 189.605 kb on + strand, within Atu3179 at 189.684 kb on + strand, within Atu3179 at 189.689 kb on + strand, within Atu3179 at 189.781 kb on - strand, within Atu3179 at 189.887 kb on - strand at 189.913 kb on + strand at 189.961 kb on + strand at 189.961 kb on + strand at 189.978 kb on + strand at 189.981 kb on - strand at 189.983 kb on + strand at 190.117 kb on - strand, within Atu3180 at 190.139 kb on - strand, within Atu3180 at 190.172 kb on - strand, within Atu3180 at 190.208 kb on + strand, within Atu3180 at 190.217 kb on - strand, within Atu3180 at 190.234 kb on + strand, within Atu3180 at 190.268 kb on + strand, within Atu3180 at 190.295 kb on - strand, within Atu3180 at 190.397 kb on + strand, within Atu3180 at 190.504 kb on + strand, within Atu3180 at 190.542 kb on + strand, within Atu3180 at 190.603 kb on - strand, within Atu3180 at 190.654 kb on + strand, within Atu3180 at 190.664 kb on + strand at 190.669 kb on + strand at 190.678 kb on + strand at 190.678 kb on + strand at 190.861 kb on + strand at 190.861 kb on + strand at 190.861 kb on + strand at 190.869 kb on - strand at 190.869 kb on - strand at 190.878 kb on + strand at 190.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 9 mM remove 188,059 + +0.5 188,067 - +0.4 188,120 + Atu3178 0.11 -1.1 188,133 - Atu3178 0.12 -2.0 188,134 - Atu3178 0.12 -0.3 188,139 - Atu3178 0.13 -0.2 188,207 - Atu3178 0.20 -0.2 188,207 - Atu3178 0.20 +0.0 188,216 - Atu3178 0.20 -0.8 188,495 + Atu3178 0.48 -0.6 188,503 - Atu3178 0.49 -0.1 188,691 + Atu3178 0.67 +0.1 188,699 - Atu3178 0.68 +0.2 188,699 - Atu3178 0.68 -0.5 188,699 - Atu3178 0.68 -1.0 188,777 - Atu3178 0.76 -0.6 188,932 - -0.3 188,946 + +0.4 188,946 + +0.1 188,954 - -0.7 189,020 - -0.1 189,020 - -0.7 189,020 - -0.3 189,020 - -1.2 189,058 + +0.1 189,066 - -0.4 189,066 - -0.1 189,116 - -0.1 189,191 - Atu3179 0.16 -0.6 189,196 - Atu3179 0.17 +0.0 189,240 + Atu3179 0.22 +0.1 189,240 + Atu3179 0.22 +0.4 189,248 - Atu3179 0.23 -0.0 189,259 + Atu3179 0.24 +0.6 189,259 + Atu3179 0.24 +0.1 189,262 - Atu3179 0.24 +0.1 189,267 - Atu3179 0.25 -0.4 189,267 - Atu3179 0.25 +0.1 189,349 - Atu3179 0.34 -0.1 189,398 - Atu3179 0.40 -0.2 189,424 - Atu3179 0.43 +0.6 189,456 - Atu3179 0.47 +0.3 189,484 + Atu3179 0.50 +0.2 189,600 + Atu3179 0.63 -0.2 189,605 + Atu3179 0.64 +0.3 189,684 + Atu3179 0.73 +0.2 189,689 + Atu3179 0.74 -0.6 189,781 - Atu3179 0.84 -0.8 189,887 - +1.0 189,913 + -0.6 189,961 + +0.5 189,961 + -0.0 189,978 + -0.4 189,981 - +0.1 189,983 + -0.2 190,117 - Atu3180 0.25 +0.7 190,139 - Atu3180 0.27 -0.7 190,172 - Atu3180 0.31 -0.3 190,208 + Atu3180 0.36 -0.7 190,217 - Atu3180 0.37 -0.5 190,234 + Atu3180 0.39 -0.4 190,268 + Atu3180 0.43 +0.3 190,295 - Atu3180 0.46 -0.2 190,397 + Atu3180 0.58 -0.1 190,504 + Atu3180 0.71 +0.0 190,542 + Atu3180 0.76 -0.8 190,603 - Atu3180 0.83 +0.1 190,654 + Atu3180 0.89 +0.4 190,664 + -1.0 190,669 + +0.1 190,678 + +1.0 190,678 + +0.8 190,861 + +0.7 190,861 + -0.1 190,861 + +0.1 190,869 - -0.4 190,869 - +0.1 190,878 + +0.7 190,886 - -0.5
Or see this region's nucleotide sequence