Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5029 and Atu5030 are separated by 160 nucleotides Atu5030 and Atu5031 are separated by 60 nucleotides Atu5031 and Atu5032 are separated by 195 nucleotides Atu5032 and Atu5033 are separated by 31 nucleotides
Atu5029: Atu5029 - hypothetical protein, at 29,924 to 30,694
Atu5029
Atu5030: Atu5030 - hypothetical protein, at 30,855 to 31,397
Atu5030
Atu5031: Atu5031 - hypothetical protein, at 31,458 to 32,576
Atu5031
Atu5032: Atu5032 - hypothetical protein, at 32,772 to 33,533
Atu5032
Atu5033: Atu5033 - hypothetical protein, at 33,565 to 34,635
Atu5033
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 30.562 kb on - strand, within Atu5029 at 30.581 kb on + strand, within Atu5029 at 30.644 kb on - strand at 30.706 kb on - strand at 30.706 kb on - strand at 30.725 kb on + strand at 30.760 kb on - strand at 30.762 kb on + strand at 30.853 kb on + strand at 31.006 kb on + strand, within Atu5030 at 31.006 kb on + strand, within Atu5030 at 31.006 kb on + strand, within Atu5030 at 31.016 kb on + strand, within Atu5030 at 31.016 kb on + strand, within Atu5030 at 31.019 kb on - strand, within Atu5030 at 31.027 kb on + strand, within Atu5030 at 31.050 kb on + strand, within Atu5030 at 31.064 kb on + strand, within Atu5030 at 31.076 kb on + strand, within Atu5030 at 31.084 kb on - strand, within Atu5030 at 31.132 kb on + strand, within Atu5030 at 31.133 kb on - strand, within Atu5030 at 31.191 kb on - strand, within Atu5030 at 31.193 kb on + strand, within Atu5030 at 31.201 kb on - strand, within Atu5030 at 31.214 kb on - strand, within Atu5030 at 31.233 kb on - strand, within Atu5030 at 31.238 kb on - strand, within Atu5030 at 31.266 kb on + strand, within Atu5030 at 31.321 kb on + strand, within Atu5030 at 31.356 kb on + strand at 31.371 kb on + strand at 31.402 kb on - strand at 31.617 kb on + strand, within Atu5031 at 31.624 kb on - strand, within Atu5031 at 31.632 kb on - strand, within Atu5031 at 31.632 kb on - strand, within Atu5031 at 31.646 kb on - strand, within Atu5031 at 31.751 kb on - strand, within Atu5031 at 31.919 kb on - strand, within Atu5031 at 31.928 kb on - strand, within Atu5031 at 31.930 kb on - strand, within Atu5031 at 31.938 kb on + strand, within Atu5031 at 32.056 kb on - strand, within Atu5031 at 32.128 kb on + strand, within Atu5031 at 32.128 kb on + strand, within Atu5031 at 32.128 kb on + strand, within Atu5031 at 32.252 kb on + strand, within Atu5031 at 32.269 kb on + strand, within Atu5031 at 32.274 kb on + strand, within Atu5031 at 32.274 kb on + strand, within Atu5031 at 32.275 kb on + strand, within Atu5031 at 32.275 kb on + strand, within Atu5031 at 32.282 kb on - strand, within Atu5031 at 32.283 kb on - strand, within Atu5031 at 32.322 kb on + strand, within Atu5031 at 32.362 kb on + strand, within Atu5031 at 32.370 kb on - strand, within Atu5031 at 32.391 kb on - strand, within Atu5031 at 32.487 kb on - strand at 32.493 kb on + strand at 32.498 kb on + strand at 32.530 kb on - strand at 32.588 kb on + strand at 32.588 kb on + strand at 32.596 kb on - strand at 32.630 kb on + strand at 32.657 kb on + strand at 32.768 kb on + strand at 32.778 kb on - strand at 32.783 kb on + strand at 32.783 kb on + strand at 32.783 kb on + strand at 32.783 kb on + strand at 32.791 kb on - strand at 32.791 kb on - strand at 32.791 kb on - strand at 32.791 kb on - strand at 32.836 kb on + strand at 32.858 kb on + strand, within Atu5032 at 32.858 kb on + strand, within Atu5032 at 32.866 kb on - strand, within Atu5032 at 32.866 kb on - strand, within Atu5032 at 32.928 kb on - strand, within Atu5032 at 32.928 kb on - strand, within Atu5032 at 32.957 kb on - strand, within Atu5032 at 32.957 kb on - strand, within Atu5032 at 33.015 kb on + strand, within Atu5032 at 33.023 kb on - strand, within Atu5032 at 33.023 kb on - strand, within Atu5032 at 33.192 kb on + strand, within Atu5032 at 33.192 kb on + strand, within Atu5032 at 33.192 kb on + strand, within Atu5032 at 33.200 kb on - strand, within Atu5032 at 33.200 kb on - strand, within Atu5032 at 33.200 kb on - strand, within Atu5032 at 33.219 kb on - strand, within Atu5032 at 33.224 kb on - strand, within Atu5032 at 33.269 kb on + strand, within Atu5032 at 33.303 kb on - strand, within Atu5032 at 33.337 kb on + strand, within Atu5032 at 33.340 kb on + strand, within Atu5032 at 33.340 kb on - strand, within Atu5032 at 33.342 kb on + strand, within Atu5032 at 33.342 kb on + strand, within Atu5032 at 33.342 kb on + strand, within Atu5032 at 33.347 kb on + strand, within Atu5032 at 33.347 kb on + strand, within Atu5032 at 33.347 kb on + strand, within Atu5032 at 33.347 kb on + strand, within Atu5032 at 33.348 kb on - strand, within Atu5032 at 33.350 kb on - strand, within Atu5032 at 33.350 kb on - strand, within Atu5032 at 33.350 kb on - strand, within Atu5032 at 33.350 kb on - strand, within Atu5032 at 33.350 kb on - strand, within Atu5032 at 33.355 kb on - strand, within Atu5032 at 33.395 kb on + strand, within Atu5032 at 33.433 kb on + strand, within Atu5032 at 33.453 kb on - strand, within Atu5032 at 33.462 kb on + strand at 33.500 kb on + strand at 33.529 kb on + strand at 33.534 kb on + strand at 33.537 kb on - strand at 33.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 30,562 - Atu5029 0.83 +0.1 30,581 + Atu5029 0.85 +0.5 30,644 - -0.1 30,706 - -0.1 30,706 - -0.5 30,725 + -0.7 30,760 - -0.4 30,762 + +0.0 30,853 + -1.8 31,006 + Atu5030 0.28 +0.9 31,006 + Atu5030 0.28 -1.2 31,006 + Atu5030 0.28 -0.4 31,016 + Atu5030 0.30 +1.6 31,016 + Atu5030 0.30 +0.0 31,019 - Atu5030 0.30 -0.1 31,027 + Atu5030 0.32 -0.9 31,050 + Atu5030 0.36 -1.7 31,064 + Atu5030 0.38 -0.1 31,076 + Atu5030 0.41 -0.1 31,084 - Atu5030 0.42 -1.3 31,132 + Atu5030 0.51 +0.4 31,133 - Atu5030 0.51 -1.0 31,191 - Atu5030 0.62 +0.6 31,193 + Atu5030 0.62 +0.4 31,201 - Atu5030 0.64 -1.1 31,214 - Atu5030 0.66 +0.8 31,233 - Atu5030 0.70 +0.1 31,238 - Atu5030 0.71 -0.1 31,266 + Atu5030 0.76 +0.9 31,321 + Atu5030 0.86 -0.2 31,356 + -1.1 31,371 + -0.9 31,402 - +0.3 31,617 + Atu5031 0.14 +0.1 31,624 - Atu5031 0.15 -1.4 31,632 - Atu5031 0.16 -0.6 31,632 - Atu5031 0.16 +0.1 31,646 - Atu5031 0.17 +0.3 31,751 - Atu5031 0.26 -0.3 31,919 - Atu5031 0.41 -0.1 31,928 - Atu5031 0.42 -0.3 31,930 - Atu5031 0.42 +0.4 31,938 + Atu5031 0.43 -0.7 32,056 - Atu5031 0.53 -0.2 32,128 + Atu5031 0.60 -0.7 32,128 + Atu5031 0.60 -0.3 32,128 + Atu5031 0.60 +0.3 32,252 + Atu5031 0.71 -1.1 32,269 + Atu5031 0.72 -0.5 32,274 + Atu5031 0.73 +0.5 32,274 + Atu5031 0.73 +0.7 32,275 + Atu5031 0.73 -0.6 32,275 + Atu5031 0.73 +0.2 32,282 - Atu5031 0.74 -0.9 32,283 - Atu5031 0.74 -1.2 32,322 + Atu5031 0.77 -1.0 32,362 + Atu5031 0.81 -1.0 32,370 - Atu5031 0.82 -0.7 32,391 - Atu5031 0.83 +0.0 32,487 - +0.4 32,493 + -1.4 32,498 + -0.7 32,530 - -0.9 32,588 + -1.8 32,588 + -1.7 32,596 - -1.1 32,630 + +0.2 32,657 + -1.0 32,768 + +1.5 32,778 - +0.0 32,783 + -0.3 32,783 + +0.3 32,783 + -0.2 32,783 + +1.2 32,791 - -0.4 32,791 - +0.3 32,791 - -0.1 32,791 - +0.2 32,836 + -1.6 32,858 + Atu5032 0.11 -0.1 32,858 + Atu5032 0.11 +0.1 32,866 - Atu5032 0.12 -0.1 32,866 - Atu5032 0.12 +0.2 32,928 - Atu5032 0.20 +0.1 32,928 - Atu5032 0.20 -0.1 32,957 - Atu5032 0.24 +0.6 32,957 - Atu5032 0.24 +0.4 33,015 + Atu5032 0.32 +0.4 33,023 - Atu5032 0.33 +0.6 33,023 - Atu5032 0.33 -1.1 33,192 + Atu5032 0.55 -0.1 33,192 + Atu5032 0.55 -1.1 33,192 + Atu5032 0.55 -0.5 33,200 - Atu5032 0.56 +0.8 33,200 - Atu5032 0.56 -0.3 33,200 - Atu5032 0.56 -0.1 33,219 - Atu5032 0.59 -0.1 33,224 - Atu5032 0.59 -1.7 33,269 + Atu5032 0.65 +0.7 33,303 - Atu5032 0.70 +0.2 33,337 + Atu5032 0.74 -0.2 33,340 + Atu5032 0.75 +1.3 33,340 - Atu5032 0.75 -0.6 33,342 + Atu5032 0.75 -0.0 33,342 + Atu5032 0.75 -1.2 33,342 + Atu5032 0.75 -0.2 33,347 + Atu5032 0.75 +0.5 33,347 + Atu5032 0.75 +0.1 33,347 + Atu5032 0.75 +0.1 33,347 + Atu5032 0.75 -0.0 33,348 - Atu5032 0.76 +0.2 33,350 - Atu5032 0.76 -1.0 33,350 - Atu5032 0.76 +0.6 33,350 - Atu5032 0.76 +0.3 33,350 - Atu5032 0.76 +0.6 33,350 - Atu5032 0.76 -0.2 33,355 - Atu5032 0.77 -0.2 33,395 + Atu5032 0.82 -1.8 33,433 + Atu5032 0.87 +0.6 33,453 - Atu5032 0.89 +2.5 33,462 + +0.2 33,500 + -0.8 33,529 + +0.4 33,534 + +0.3 33,537 - +0.2 33,542 - -1.1
Or see this region's nucleotide sequence