Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4732 and Atu4733 are separated by 21 nucleotides Atu4733 and Atu4734 are separated by 363 nucleotides Atu4734 and Atu4736 are separated by 408 nucleotides
Atu4732: Atu4732 - fimbrial chaperone, at 1,898,306 to 1,899,022
Atu4732
Atu4733: Atu4733 - hypothetical protein, at 1,899,044 to 1,899,541
Atu4733
Atu4734: Atu4734 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,899,905 to 1,902,694
Atu4734
Atu4736: Atu4736 - methyl-accepting chemotaxis protein, at 1,903,103 to 1,905,046
Atu4736
Position (kb)
1899
1900
1901
1902
1903 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1898.935 kb on + strand, within Atu4732 at 1898.935 kb on + strand, within Atu4732 at 1898.943 kb on - strand, within Atu4732 at 1899.040 kb on - strand at 1899.053 kb on - strand at 1899.139 kb on - strand, within Atu4733 at 1899.149 kb on + strand, within Atu4733 at 1899.188 kb on - strand, within Atu4733 at 1899.188 kb on - strand, within Atu4733 at 1899.193 kb on - strand, within Atu4733 at 1899.218 kb on - strand, within Atu4733 at 1899.218 kb on - strand, within Atu4733 at 1899.235 kb on - strand, within Atu4733 at 1899.308 kb on + strand, within Atu4733 at 1899.314 kb on - strand, within Atu4733 at 1899.316 kb on - strand, within Atu4733 at 1899.437 kb on + strand, within Atu4733 at 1899.459 kb on + strand, within Atu4733 at 1899.502 kb on - strand at 1899.515 kb on + strand at 1899.648 kb on - strand at 1899.760 kb on + strand at 1899.760 kb on + strand at 1899.768 kb on - strand at 1899.773 kb on + strand at 1899.826 kb on + strand at 1899.897 kb on + strand at 1899.927 kb on - strand at 1900.083 kb on + strand at 1900.147 kb on - strand at 1900.197 kb on - strand, within Atu4734 at 1900.234 kb on - strand, within Atu4734 at 1900.522 kb on + strand, within Atu4734 at 1900.522 kb on + strand, within Atu4734 at 1900.522 kb on + strand, within Atu4734 at 1900.522 kb on + strand, within Atu4734 at 1900.522 kb on + strand, within Atu4734 at 1900.530 kb on - strand, within Atu4734 at 1900.530 kb on - strand, within Atu4734 at 1900.530 kb on - strand, within Atu4734 at 1900.530 kb on - strand, within Atu4734 at 1900.595 kb on - strand, within Atu4734 at 1900.691 kb on - strand, within Atu4734 at 1900.808 kb on - strand, within Atu4734 at 1901.032 kb on + strand, within Atu4734 at 1901.048 kb on + strand, within Atu4734 at 1901.069 kb on - strand, within Atu4734 at 1901.072 kb on + strand, within Atu4734 at 1901.080 kb on - strand, within Atu4734 at 1901.111 kb on + strand, within Atu4734 at 1901.119 kb on - strand, within Atu4734 at 1901.124 kb on - strand, within Atu4734 at 1901.230 kb on + strand, within Atu4734 at 1901.238 kb on - strand, within Atu4734 at 1901.238 kb on - strand, within Atu4734 at 1901.238 kb on - strand, within Atu4734 at 1901.248 kb on - strand, within Atu4734 at 1901.322 kb on + strand, within Atu4734 at 1901.356 kb on + strand, within Atu4734 at 1901.424 kb on - strand, within Atu4734 at 1901.549 kb on + strand, within Atu4734 at 1901.551 kb on + strand, within Atu4734 at 1901.575 kb on + strand, within Atu4734 at 1901.641 kb on + strand, within Atu4734 at 1901.649 kb on - strand, within Atu4734 at 1901.686 kb on - strand, within Atu4734 at 1901.688 kb on - strand, within Atu4734 at 1901.764 kb on + strand, within Atu4734 at 1901.891 kb on + strand, within Atu4734 at 1901.891 kb on + strand, within Atu4734 at 1901.891 kb on + strand, within Atu4734 at 1901.891 kb on + strand, within Atu4734 at 1901.934 kb on + strand, within Atu4734 at 1902.209 kb on + strand, within Atu4734 at 1902.245 kb on - strand, within Atu4734 at 1902.249 kb on - strand, within Atu4734 at 1902.265 kb on - strand, within Atu4734 at 1902.371 kb on + strand, within Atu4734 at 1902.460 kb on + strand at 1902.468 kb on - strand at 1902.657 kb on + strand at 1902.657 kb on + strand at 1902.660 kb on - strand at 1902.688 kb on + strand at 1902.691 kb on - strand at 1902.691 kb on - strand at 1902.696 kb on - strand at 1902.696 kb on - strand at 1902.927 kb on - strand at 1903.043 kb on + strand at 1903.059 kb on - strand at 1903.673 kb on + strand, within Atu4736 at 1903.693 kb on + strand, within Atu4736
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 1,898,935 + Atu4732 0.88 -0.5 1,898,935 + Atu4732 0.88 -0.3 1,898,943 - Atu4732 0.89 +2.2 1,899,040 - +0.0 1,899,053 - -0.6 1,899,139 - Atu4733 0.19 -0.2 1,899,149 + Atu4733 0.21 +0.3 1,899,188 - Atu4733 0.29 -0.3 1,899,188 - Atu4733 0.29 -0.6 1,899,193 - Atu4733 0.30 +0.8 1,899,218 - Atu4733 0.35 -0.6 1,899,218 - Atu4733 0.35 -0.1 1,899,235 - Atu4733 0.38 -1.0 1,899,308 + Atu4733 0.53 +0.0 1,899,314 - Atu4733 0.54 -0.4 1,899,316 - Atu4733 0.55 +0.1 1,899,437 + Atu4733 0.79 +0.1 1,899,459 + Atu4733 0.83 -1.2 1,899,502 - +0.4 1,899,515 + +0.1 1,899,648 - +0.8 1,899,760 + +0.2 1,899,760 + +0.7 1,899,768 - -0.7 1,899,773 + -0.1 1,899,826 + +3.4 1,899,897 + +4.9 1,899,927 - +5.8 1,900,083 + +5.8 1,900,147 - +5.7 1,900,197 - Atu4734 0.10 +5.2 1,900,234 - Atu4734 0.12 +5.8 1,900,522 + Atu4734 0.22 +5.8 1,900,522 + Atu4734 0.22 +6.1 1,900,522 + Atu4734 0.22 +5.9 1,900,522 + Atu4734 0.22 +6.1 1,900,522 + Atu4734 0.22 +5.6 1,900,530 - Atu4734 0.22 +6.0 1,900,530 - Atu4734 0.22 +5.9 1,900,530 - Atu4734 0.22 +5.8 1,900,530 - Atu4734 0.22 +5.5 1,900,595 - Atu4734 0.25 +5.2 1,900,691 - Atu4734 0.28 +5.9 1,900,808 - Atu4734 0.32 +6.4 1,901,032 + Atu4734 0.40 +6.0 1,901,048 + Atu4734 0.41 +5.7 1,901,069 - Atu4734 0.42 +5.9 1,901,072 + Atu4734 0.42 +5.8 1,901,080 - Atu4734 0.42 +6.0 1,901,111 + Atu4734 0.43 +5.8 1,901,119 - Atu4734 0.44 +5.9 1,901,124 - Atu4734 0.44 +5.7 1,901,230 + Atu4734 0.47 +6.0 1,901,238 - Atu4734 0.48 +6.1 1,901,238 - Atu4734 0.48 +5.2 1,901,238 - Atu4734 0.48 +5.7 1,901,248 - Atu4734 0.48 +6.0 1,901,322 + Atu4734 0.51 +5.9 1,901,356 + Atu4734 0.52 +5.6 1,901,424 - Atu4734 0.54 +6.0 1,901,549 + Atu4734 0.59 +6.1 1,901,551 + Atu4734 0.59 +5.8 1,901,575 + Atu4734 0.60 +5.5 1,901,641 + Atu4734 0.62 +5.6 1,901,649 - Atu4734 0.63 +5.9 1,901,686 - Atu4734 0.64 +5.9 1,901,688 - Atu4734 0.64 +5.5 1,901,764 + Atu4734 0.67 +5.8 1,901,891 + Atu4734 0.71 +5.8 1,901,891 + Atu4734 0.71 +5.7 1,901,891 + Atu4734 0.71 +5.8 1,901,891 + Atu4734 0.71 +6.0 1,901,934 + Atu4734 0.73 +5.9 1,902,209 + Atu4734 0.83 +5.7 1,902,245 - Atu4734 0.84 +5.5 1,902,249 - Atu4734 0.84 +5.8 1,902,265 - Atu4734 0.85 +5.6 1,902,371 + Atu4734 0.88 +6.0 1,902,460 + +5.9 1,902,468 - +4.9 1,902,657 + +1.1 1,902,657 + +1.2 1,902,660 - +5.3 1,902,688 + +0.5 1,902,691 - +5.3 1,902,691 - +5.3 1,902,696 - +0.9 1,902,696 - -0.1 1,902,927 - -0.8 1,903,043 + -0.4 1,903,059 - -1.2 1,903,673 + Atu4736 0.29 +0.9 1,903,693 + Atu4736 0.30 +0.3
Or see this region's nucleotide sequence