Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3292 and Atu3293 are separated by 7 nucleotides Atu3293 and Atu3294 are separated by 49 nucleotides Atu3294 and Atu3295 are separated by 179 nucleotides
Atu3292: Atu3292 - alanine racemase, at 317,717 to 318,883
Atu3292
Atu3293: Atu3293 - D-amino acid dehydrogenase small subunit, at 318,891 to 320,147
Atu3293
Atu3294: Atu3294 - GntR family transcriptional regulator, at 320,197 to 320,907
Atu3294
Atu3295: Atu3295 - hypothetical protein, at 321,087 to 321,599
Atu3295
Position (kb) 318
319
320
321 Strain fitness (log2 ratio) -1
0
1 at 318.050 kb on + strand, within Atu3292 at 318.068 kb on - strand, within Atu3292 at 318.081 kb on + strand, within Atu3292 at 318.094 kb on - strand, within Atu3292 at 318.158 kb on - strand, within Atu3292 at 318.162 kb on + strand, within Atu3292 at 318.162 kb on + strand, within Atu3292 at 318.170 kb on - strand, within Atu3292 at 318.170 kb on - strand, within Atu3292 at 318.196 kb on + strand, within Atu3292 at 318.221 kb on + strand, within Atu3292 at 318.223 kb on - strand, within Atu3292 at 318.246 kb on + strand, within Atu3292 at 318.268 kb on + strand, within Atu3292 at 318.383 kb on + strand, within Atu3292 at 318.383 kb on + strand, within Atu3292 at 318.386 kb on - strand, within Atu3292 at 318.550 kb on + strand, within Atu3292 at 318.568 kb on - strand, within Atu3292 at 318.614 kb on + strand, within Atu3292 at 318.622 kb on - strand, within Atu3292 at 318.627 kb on - strand, within Atu3292 at 318.671 kb on + strand, within Atu3292 at 318.684 kb on - strand, within Atu3292 at 318.699 kb on - strand, within Atu3292 at 318.730 kb on + strand, within Atu3292 at 318.735 kb on + strand, within Atu3292 at 318.735 kb on + strand, within Atu3292 at 318.747 kb on + strand, within Atu3292 at 318.947 kb on + strand at 318.954 kb on + strand at 319.059 kb on + strand, within Atu3293 at 319.097 kb on + strand, within Atu3293 at 319.153 kb on + strand, within Atu3293 at 319.273 kb on - strand, within Atu3293 at 319.303 kb on - strand, within Atu3293 at 319.326 kb on - strand, within Atu3293 at 319.355 kb on - strand, within Atu3293 at 319.423 kb on + strand, within Atu3293 at 319.489 kb on + strand, within Atu3293 at 319.489 kb on + strand, within Atu3293 at 319.509 kb on - strand, within Atu3293 at 319.574 kb on + strand, within Atu3293 at 319.686 kb on - strand, within Atu3293 at 319.790 kb on + strand, within Atu3293 at 319.924 kb on + strand, within Atu3293 at 320.213 kb on + strand at 320.225 kb on + strand at 320.252 kb on - strand at 320.293 kb on + strand, within Atu3294 at 320.372 kb on + strand, within Atu3294 at 320.397 kb on - strand, within Atu3294 at 320.464 kb on - strand, within Atu3294 at 320.491 kb on - strand, within Atu3294 at 320.630 kb on - strand, within Atu3294 at 320.656 kb on - strand, within Atu3294 at 320.690 kb on + strand, within Atu3294 at 320.704 kb on - strand, within Atu3294 at 320.704 kb on - strand, within Atu3294 at 320.704 kb on - strand, within Atu3294 at 320.704 kb on - strand, within Atu3294 at 320.714 kb on - strand, within Atu3294 at 320.733 kb on - strand, within Atu3294 at 320.735 kb on + strand, within Atu3294 at 320.743 kb on - strand, within Atu3294 at 320.743 kb on - strand, within Atu3294 at 320.745 kb on + strand, within Atu3294 at 320.895 kb on + strand at 320.976 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 318,050 + Atu3292 0.29 -0.0 318,068 - Atu3292 0.30 +0.6 318,081 + Atu3292 0.31 -1.7 318,094 - Atu3292 0.32 -0.3 318,158 - Atu3292 0.38 -0.4 318,162 + Atu3292 0.38 -0.0 318,162 + Atu3292 0.38 -0.1 318,170 - Atu3292 0.39 -0.8 318,170 - Atu3292 0.39 +0.0 318,196 + Atu3292 0.41 -0.0 318,221 + Atu3292 0.43 -0.7 318,223 - Atu3292 0.43 -1.4 318,246 + Atu3292 0.45 -0.1 318,268 + Atu3292 0.47 -0.6 318,383 + Atu3292 0.57 -0.1 318,383 + Atu3292 0.57 +0.1 318,386 - Atu3292 0.57 -1.2 318,550 + Atu3292 0.71 -0.0 318,568 - Atu3292 0.73 -0.1 318,614 + Atu3292 0.77 +0.2 318,622 - Atu3292 0.78 +0.4 318,627 - Atu3292 0.78 +0.1 318,671 + Atu3292 0.82 +0.0 318,684 - Atu3292 0.83 -0.2 318,699 - Atu3292 0.84 -0.9 318,730 + Atu3292 0.87 -1.2 318,735 + Atu3292 0.87 +0.1 318,735 + Atu3292 0.87 +0.2 318,747 + Atu3292 0.88 +0.6 318,947 + -0.6 318,954 + -0.2 319,059 + Atu3293 0.13 +0.1 319,097 + Atu3293 0.16 -0.1 319,153 + Atu3293 0.21 +0.0 319,273 - Atu3293 0.30 +0.5 319,303 - Atu3293 0.33 -0.7 319,326 - Atu3293 0.35 +0.2 319,355 - Atu3293 0.37 +0.3 319,423 + Atu3293 0.42 +0.4 319,489 + Atu3293 0.48 +1.1 319,489 + Atu3293 0.48 -1.2 319,509 - Atu3293 0.49 +0.3 319,574 + Atu3293 0.54 +0.0 319,686 - Atu3293 0.63 -0.0 319,790 + Atu3293 0.72 -1.1 319,924 + Atu3293 0.82 +0.3 320,213 + +1.0 320,225 + +0.8 320,252 - +0.1 320,293 + Atu3294 0.14 -0.3 320,372 + Atu3294 0.25 -1.0 320,397 - Atu3294 0.28 +0.5 320,464 - Atu3294 0.38 -0.3 320,491 - Atu3294 0.41 -1.7 320,630 - Atu3294 0.61 +0.5 320,656 - Atu3294 0.65 -1.0 320,690 + Atu3294 0.69 -0.4 320,704 - Atu3294 0.71 +0.1 320,704 - Atu3294 0.71 -1.3 320,704 - Atu3294 0.71 -0.1 320,704 - Atu3294 0.71 +0.0 320,714 - Atu3294 0.73 +0.2 320,733 - Atu3294 0.75 -1.1 320,735 + Atu3294 0.76 -1.0 320,743 - Atu3294 0.77 +0.1 320,743 - Atu3294 0.77 +0.5 320,745 + Atu3294 0.77 -0.1 320,895 + +0.2 320,976 + +0.4
Or see this region's nucleotide sequence