Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0402 and Atu0403 overlap by 4 nucleotides Atu0403 and Atu0404 are separated by 55 nucleotides Atu0404 and Atu0405 are separated by 130 nucleotides
Atu0402: Atu0402 - hypothetical protein, at 396,686 to 397,651
Atu0402
Atu0403: Atu0403 - hypothetical protein, at 397,648 to 398,376
Atu0403
Atu0404: Atu0404 - glucose-6-phosphate isomerase, at 398,432 to 400,057
Atu0404
Atu0405: Atu0405 - long-chain-fatty-acid-CoA-ligase, at 400,188 to 401,900
Atu0405
Position (kb) 398
399
400
401 Strain fitness (log2 ratio) -1
0
1
2 at 397.446 kb on + strand, within Atu0402 at 397.527 kb on - strand, within Atu0402 at 397.527 kb on - strand, within Atu0402 at 397.527 kb on - strand, within Atu0402 at 397.527 kb on - strand, within Atu0402 at 397.561 kb on - strand at 397.585 kb on + strand at 397.593 kb on - strand at 397.619 kb on + strand at 397.676 kb on + strand at 397.694 kb on + strand at 397.712 kb on - strand at 397.732 kb on + strand, within Atu0403 at 397.830 kb on + strand, within Atu0403 at 397.838 kb on - strand, within Atu0403 at 397.979 kb on + strand, within Atu0403 at 397.992 kb on - strand, within Atu0403 at 397.992 kb on - strand, within Atu0403 at 397.997 kb on - strand, within Atu0403 at 398.036 kb on + strand, within Atu0403 at 398.044 kb on + strand, within Atu0403 at 398.083 kb on + strand, within Atu0403 at 398.093 kb on + strand, within Atu0403 at 398.178 kb on + strand, within Atu0403 at 398.186 kb on - strand, within Atu0403 at 398.221 kb on + strand, within Atu0403 at 398.221 kb on + strand, within Atu0403 at 398.229 kb on - strand, within Atu0403 at 398.274 kb on - strand, within Atu0403 at 398.274 kb on - strand, within Atu0403 at 398.274 kb on - strand, within Atu0403 at 398.274 kb on - strand, within Atu0403 at 398.349 kb on + strand at 398.454 kb on + strand at 400.066 kb on - strand at 400.257 kb on - strand at 400.306 kb on + strand at 400.314 kb on - strand at 400.410 kb on - strand, within Atu0405 at 400.412 kb on + strand, within Atu0405 at 400.445 kb on + strand, within Atu0405 at 400.506 kb on + strand, within Atu0405 at 400.572 kb on - strand, within Atu0405 at 400.614 kb on - strand, within Atu0405 at 400.614 kb on - strand, within Atu0405 at 400.614 kb on - strand, within Atu0405 at 400.641 kb on + strand, within Atu0405 at 400.660 kb on + strand, within Atu0405 at 400.709 kb on + strand, within Atu0405 at 400.709 kb on + strand, within Atu0405 at 400.776 kb on - strand, within Atu0405 at 400.779 kb on + strand, within Atu0405 at 400.787 kb on - strand, within Atu0405 at 400.844 kb on + strand, within Atu0405 at 400.844 kb on - strand, within Atu0405 at 400.844 kb on - strand, within Atu0405 at 400.849 kb on - strand, within Atu0405 at 401.015 kb on + strand, within Atu0405 at 401.015 kb on + strand, within Atu0405 at 401.030 kb on + strand, within Atu0405 at 401.030 kb on + strand, within Atu0405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 397,446 + Atu0402 0.79 +0.2 397,527 - Atu0402 0.87 -0.5 397,527 - Atu0402 0.87 +0.4 397,527 - Atu0402 0.87 +0.2 397,527 - Atu0402 0.87 +0.1 397,561 - +0.1 397,585 + +0.2 397,593 - +0.5 397,619 + -0.2 397,676 + +0.5 397,694 + +2.0 397,712 - +0.4 397,732 + Atu0403 0.12 -0.0 397,830 + Atu0403 0.25 +0.1 397,838 - Atu0403 0.26 -0.0 397,979 + Atu0403 0.45 -0.1 397,992 - Atu0403 0.47 -0.1 397,992 - Atu0403 0.47 +0.5 397,997 - Atu0403 0.48 -0.6 398,036 + Atu0403 0.53 +0.1 398,044 + Atu0403 0.54 +0.2 398,083 + Atu0403 0.60 -1.7 398,093 + Atu0403 0.61 -0.4 398,178 + Atu0403 0.73 -0.1 398,186 - Atu0403 0.74 -1.2 398,221 + Atu0403 0.79 +0.1 398,221 + Atu0403 0.79 -0.4 398,229 - Atu0403 0.80 +0.4 398,274 - Atu0403 0.86 +0.6 398,274 - Atu0403 0.86 +0.4 398,274 - Atu0403 0.86 +0.1 398,274 - Atu0403 0.86 -0.3 398,349 + +0.7 398,454 + -0.2 400,066 - +0.9 400,257 - -0.2 400,306 + +0.1 400,314 - -0.5 400,410 - Atu0405 0.13 -0.7 400,412 + Atu0405 0.13 -0.1 400,445 + Atu0405 0.15 -0.4 400,506 + Atu0405 0.19 +1.3 400,572 - Atu0405 0.22 -0.0 400,614 - Atu0405 0.25 +0.3 400,614 - Atu0405 0.25 -0.8 400,614 - Atu0405 0.25 +0.3 400,641 + Atu0405 0.26 +0.3 400,660 + Atu0405 0.28 -0.1 400,709 + Atu0405 0.30 +0.3 400,709 + Atu0405 0.30 +1.1 400,776 - Atu0405 0.34 +0.4 400,779 + Atu0405 0.35 -0.7 400,787 - Atu0405 0.35 -0.2 400,844 + Atu0405 0.38 -0.1 400,844 - Atu0405 0.38 -0.3 400,844 - Atu0405 0.38 -0.2 400,849 - Atu0405 0.39 -0.3 401,015 + Atu0405 0.48 +0.1 401,015 + Atu0405 0.48 +0.2 401,030 + Atu0405 0.49 -0.8 401,030 + Atu0405 0.49 -0.8
Or see this region's nucleotide sequence