Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0285 and Atu0286 are separated by 3 nucleotides Atu0286 and Atu0287 overlap by 1 nucleotides
Atu0285: Atu0285 - hypothetical protein, at 278,400 to 279,494
Atu0285
Atu0286: Atu0286 - hypothetical protein, at 279,498 to 280,619
Atu0286
Atu0287: Atu0287 - hypothetical protein, at 280,619 to 282,160
Atu0287
Position (kb)
279
280
281 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 278.509 kb on - strand at 278.592 kb on + strand, within Atu0285 at 278.609 kb on - strand, within Atu0285 at 278.619 kb on + strand, within Atu0285 at 278.690 kb on + strand, within Atu0285 at 278.692 kb on + strand, within Atu0285 at 278.692 kb on + strand, within Atu0285 at 278.700 kb on - strand, within Atu0285 at 278.700 kb on - strand, within Atu0285 at 278.700 kb on - strand, within Atu0285 at 278.751 kb on - strand, within Atu0285 at 278.826 kb on + strand, within Atu0285 at 278.836 kb on + strand, within Atu0285 at 278.890 kb on - strand, within Atu0285 at 279.001 kb on + strand, within Atu0285 at 279.026 kb on + strand, within Atu0285 at 279.054 kb on + strand, within Atu0285 at 279.101 kb on + strand, within Atu0285 at 279.101 kb on + strand, within Atu0285 at 279.174 kb on + strand, within Atu0285 at 279.174 kb on + strand, within Atu0285 at 279.238 kb on + strand, within Atu0285 at 279.570 kb on + strand at 279.583 kb on + strand at 279.603 kb on - strand at 279.630 kb on - strand, within Atu0286 at 279.647 kb on - strand, within Atu0286 at 279.728 kb on + strand, within Atu0286 at 279.753 kb on + strand, within Atu0286 at 279.761 kb on - strand, within Atu0286 at 279.772 kb on + strand, within Atu0286 at 279.772 kb on + strand, within Atu0286 at 279.772 kb on + strand, within Atu0286 at 279.772 kb on + strand, within Atu0286 at 279.772 kb on + strand, within Atu0286 at 279.772 kb on + strand, within Atu0286 at 279.780 kb on - strand, within Atu0286 at 279.780 kb on - strand, within Atu0286 at 279.780 kb on - strand, within Atu0286 at 279.801 kb on + strand, within Atu0286 at 279.821 kb on + strand, within Atu0286 at 279.857 kb on - strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.893 kb on + strand, within Atu0286 at 279.901 kb on - strand, within Atu0286 at 279.901 kb on - strand, within Atu0286 at 279.901 kb on - strand, within Atu0286 at 279.911 kb on - strand, within Atu0286 at 279.921 kb on - strand, within Atu0286 at 280.004 kb on - strand, within Atu0286 at 280.142 kb on + strand, within Atu0286 at 280.142 kb on + strand, within Atu0286 at 280.208 kb on + strand, within Atu0286 at 280.230 kb on - strand, within Atu0286 at 280.258 kb on + strand, within Atu0286 at 280.258 kb on + strand, within Atu0286 at 280.266 kb on - strand, within Atu0286 at 280.266 kb on - strand, within Atu0286 at 280.266 kb on - strand, within Atu0286 at 280.332 kb on + strand, within Atu0286 at 280.332 kb on + strand, within Atu0286 at 280.332 kb on + strand, within Atu0286 at 280.332 kb on + strand, within Atu0286 at 280.332 kb on + strand, within Atu0286 at 280.332 kb on + strand, within Atu0286 at 280.340 kb on - strand, within Atu0286 at 280.340 kb on - strand, within Atu0286 at 280.340 kb on - strand, within Atu0286 at 280.415 kb on + strand, within Atu0286 at 280.421 kb on - strand, within Atu0286 at 280.453 kb on - strand, within Atu0286 at 280.461 kb on + strand, within Atu0286 at 280.500 kb on - strand, within Atu0286 at 280.502 kb on + strand, within Atu0286 at 280.507 kb on + strand at 280.510 kb on - strand at 280.510 kb on - strand at 280.515 kb on - strand at 280.538 kb on - strand at 280.681 kb on - strand at 280.734 kb on - strand at 280.734 kb on - strand at 280.764 kb on - strand at 280.777 kb on - strand, within Atu0287 at 280.815 kb on + strand, within Atu0287 at 280.815 kb on + strand, within Atu0287 at 280.875 kb on - strand, within Atu0287 at 280.896 kb on - strand, within Atu0287 at 281.006 kb on + strand, within Atu0287 at 281.147 kb on + strand, within Atu0287 at 281.147 kb on - strand, within Atu0287 at 281.245 kb on + strand, within Atu0287 at 281.305 kb on + strand, within Atu0287 at 281.305 kb on - strand, within Atu0287 at 281.364 kb on - strand, within Atu0287 at 281.385 kb on + strand, within Atu0287 at 281.407 kb on + strand, within Atu0287 at 281.407 kb on + strand, within Atu0287 at 281.409 kb on - strand, within Atu0287 at 281.446 kb on + strand, within Atu0287 at 281.487 kb on + strand, within Atu0287 at 281.492 kb on - strand, within Atu0287 at 281.496 kb on - strand, within Atu0287 at 281.501 kb on - strand, within Atu0287
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 278,509 - -1.3 278,592 + Atu0285 0.18 -1.2 278,609 - Atu0285 0.19 -1.3 278,619 + Atu0285 0.20 -0.9 278,690 + Atu0285 0.26 -0.9 278,692 + Atu0285 0.27 -1.0 278,692 + Atu0285 0.27 -0.5 278,700 - Atu0285 0.27 -0.8 278,700 - Atu0285 0.27 -1.0 278,700 - Atu0285 0.27 -1.8 278,751 - Atu0285 0.32 -0.6 278,826 + Atu0285 0.39 -0.9 278,836 + Atu0285 0.40 -1.3 278,890 - Atu0285 0.45 -0.8 279,001 + Atu0285 0.55 -1.0 279,026 + Atu0285 0.57 -1.1 279,054 + Atu0285 0.60 -1.0 279,101 + Atu0285 0.64 -1.2 279,101 + Atu0285 0.64 -1.3 279,174 + Atu0285 0.71 -1.9 279,174 + Atu0285 0.71 -0.9 279,238 + Atu0285 0.77 -1.0 279,570 + -1.9 279,583 + -1.1 279,603 - -0.7 279,630 - Atu0286 0.12 -0.0 279,647 - Atu0286 0.13 -1.3 279,728 + Atu0286 0.20 -1.2 279,753 + Atu0286 0.23 -0.1 279,761 - Atu0286 0.23 -0.9 279,772 + Atu0286 0.24 -0.1 279,772 + Atu0286 0.24 -0.9 279,772 + Atu0286 0.24 -1.1 279,772 + Atu0286 0.24 -2.2 279,772 + Atu0286 0.24 -0.9 279,772 + Atu0286 0.24 -1.8 279,780 - Atu0286 0.25 -1.2 279,780 - Atu0286 0.25 -0.5 279,780 - Atu0286 0.25 -0.5 279,801 + Atu0286 0.27 -0.9 279,821 + Atu0286 0.29 -0.6 279,857 - Atu0286 0.32 -1.9 279,893 + Atu0286 0.35 -0.9 279,893 + Atu0286 0.35 -0.9 279,893 + Atu0286 0.35 -0.8 279,893 + Atu0286 0.35 +0.2 279,893 + Atu0286 0.35 -2.1 279,893 + Atu0286 0.35 -1.1 279,893 + Atu0286 0.35 -2.5 279,901 - Atu0286 0.36 -0.2 279,901 - Atu0286 0.36 -1.5 279,901 - Atu0286 0.36 -1.0 279,911 - Atu0286 0.37 -0.8 279,921 - Atu0286 0.38 +0.8 280,004 - Atu0286 0.45 -1.0 280,142 + Atu0286 0.57 +0.3 280,142 + Atu0286 0.57 -0.5 280,208 + Atu0286 0.63 -1.0 280,230 - Atu0286 0.65 -0.8 280,258 + Atu0286 0.68 -1.9 280,258 + Atu0286 0.68 +0.6 280,266 - Atu0286 0.68 -1.3 280,266 - Atu0286 0.68 -0.4 280,266 - Atu0286 0.68 -1.0 280,332 + Atu0286 0.74 -2.9 280,332 + Atu0286 0.74 +1.5 280,332 + Atu0286 0.74 -1.8 280,332 + Atu0286 0.74 -0.5 280,332 + Atu0286 0.74 -0.9 280,332 + Atu0286 0.74 -0.2 280,340 - Atu0286 0.75 -1.5 280,340 - Atu0286 0.75 -1.0 280,340 - Atu0286 0.75 -1.4 280,415 + Atu0286 0.82 -0.4 280,421 - Atu0286 0.82 -1.8 280,453 - Atu0286 0.85 -0.6 280,461 + Atu0286 0.86 -0.8 280,500 - Atu0286 0.89 +0.2 280,502 + Atu0286 0.89 -1.5 280,507 + -1.1 280,510 - -0.5 280,510 - -0.5 280,515 - -0.7 280,538 - -1.3 280,681 - -1.6 280,734 - -0.2 280,734 - -1.7 280,764 - -0.9 280,777 - Atu0287 0.10 -0.7 280,815 + Atu0287 0.13 -0.5 280,815 + Atu0287 0.13 -1.9 280,875 - Atu0287 0.17 -1.2 280,896 - Atu0287 0.18 -1.2 281,006 + Atu0287 0.25 -1.3 281,147 + Atu0287 0.34 +0.3 281,147 - Atu0287 0.34 -0.5 281,245 + Atu0287 0.41 +0.7 281,305 + Atu0287 0.44 -1.0 281,305 - Atu0287 0.44 -1.3 281,364 - Atu0287 0.48 -1.8 281,385 + Atu0287 0.50 -0.5 281,407 + Atu0287 0.51 -1.4 281,407 + Atu0287 0.51 -1.6 281,409 - Atu0287 0.51 -2.1 281,446 + Atu0287 0.54 -0.5 281,487 + Atu0287 0.56 -0.9 281,492 - Atu0287 0.57 -0.7 281,496 - Atu0287 0.57 -1.0 281,501 - Atu0287 0.57 +0.1
Or see this region's nucleotide sequence