Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gsk and ybaL are separated by 132 nucleotides ybaL and fsr are separated by 237 nucleotides
b0477: gsk - inosine/guanosine kinase (NCBI), at 499,349 to 500,653
gsk
b0478: ybaL - predicted transporter with NAD(P)-binding Rossmann-fold domain (NCBI), at 500,786 to 502,462
ybaL
b0479: fsr - predicted fosmidomycin efflux system (NCBI), at 502,700 to 503,920
fsr
Position (kb) 500
501
502
503 Strain fitness (log2 ratio) -2
-1
0
1 at 499.803 kb on + strand, within gsk at 499.803 kb on + strand, within gsk at 499.856 kb on - strand, within gsk at 499.913 kb on + strand, within gsk at 499.913 kb on + strand, within gsk at 499.951 kb on + strand, within gsk at 499.952 kb on + strand, within gsk at 500.002 kb on + strand, within gsk at 500.027 kb on - strand, within gsk at 500.072 kb on - strand, within gsk at 500.168 kb on + strand, within gsk at 500.176 kb on - strand, within gsk at 500.186 kb on - strand, within gsk at 500.202 kb on - strand, within gsk at 500.202 kb on - strand, within gsk at 500.302 kb on + strand, within gsk at 500.306 kb on + strand, within gsk at 500.359 kb on + strand, within gsk at 500.404 kb on + strand, within gsk at 500.471 kb on - strand, within gsk at 500.581 kb on + strand at 500.581 kb on + strand at 500.633 kb on - strand at 500.633 kb on - strand at 500.643 kb on - strand at 500.664 kb on + strand at 500.692 kb on - strand at 500.794 kb on + strand at 500.817 kb on + strand at 500.868 kb on + strand at 500.868 kb on + strand at 501.023 kb on + strand, within ybaL at 501.023 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.073 kb on - strand, within ybaL at 501.073 kb on - strand, within ybaL at 501.073 kb on - strand, within ybaL at 501.075 kb on + strand, within ybaL at 501.178 kb on + strand, within ybaL at 501.184 kb on - strand, within ybaL at 501.184 kb on - strand, within ybaL at 501.316 kb on + strand, within ybaL at 501.316 kb on + strand, within ybaL at 501.324 kb on - strand, within ybaL at 501.358 kb on - strand, within ybaL at 501.358 kb on - strand, within ybaL at 501.417 kb on + strand, within ybaL at 501.417 kb on + strand, within ybaL at 501.455 kb on + strand, within ybaL at 501.455 kb on + strand, within ybaL at 501.468 kb on + strand, within ybaL at 501.677 kb on + strand, within ybaL at 501.927 kb on - strand, within ybaL at 501.986 kb on - strand, within ybaL at 502.033 kb on - strand, within ybaL at 502.051 kb on + strand, within ybaL at 502.105 kb on + strand, within ybaL at 502.154 kb on + strand, within ybaL at 502.155 kb on - strand, within ybaL at 502.155 kb on - strand, within ybaL at 502.293 kb on - strand, within ybaL at 502.293 kb on - strand, within ybaL at 502.346 kb on + strand at 502.346 kb on + strand at 502.384 kb on - strand at 502.384 kb on - strand at 502.437 kb on - strand at 502.656 kb on + strand at 502.816 kb on - strand at 502.990 kb on - strand, within fsr at 503.020 kb on + strand, within fsr at 503.084 kb on + strand, within fsr at 503.125 kb on - strand, within fsr at 503.125 kb on - strand, within fsr at 503.134 kb on + strand, within fsr at 503.134 kb on + strand, within fsr at 503.178 kb on - strand, within fsr at 503.275 kb on - strand, within fsr at 503.288 kb on - strand, within fsr at 503.288 kb on - strand, within fsr
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 remove 499,803 + gsk b0477 0.35 -0.6 499,803 + gsk b0477 0.35 +0.0 499,856 - gsk b0477 0.39 -0.7 499,913 + gsk b0477 0.43 +1.1 499,913 + gsk b0477 0.43 -0.6 499,951 + gsk b0477 0.46 -0.3 499,952 + gsk b0477 0.46 -0.5 500,002 + gsk b0477 0.50 +0.4 500,027 - gsk b0477 0.52 -0.4 500,072 - gsk b0477 0.55 -2.3 500,168 + gsk b0477 0.63 -0.2 500,176 - gsk b0477 0.63 -0.2 500,186 - gsk b0477 0.64 -1.8 500,202 - gsk b0477 0.65 +0.3 500,202 - gsk b0477 0.65 +0.0 500,302 + gsk b0477 0.73 -0.1 500,306 + gsk b0477 0.73 -0.8 500,359 + gsk b0477 0.77 -0.1 500,404 + gsk b0477 0.81 -0.1 500,471 - gsk b0477 0.86 +0.2 500,581 + +0.5 500,581 + -0.9 500,633 - +0.2 500,633 - +0.4 500,643 - +0.2 500,664 + -0.0 500,692 - +0.6 500,794 + -1.0 500,817 + +0.2 500,868 + +0.5 500,868 + -0.4 501,023 + ybaL b0478 0.14 -1.1 501,023 + ybaL b0478 0.14 +0.7 501,065 + ybaL b0478 0.17 -0.1 501,065 + ybaL b0478 0.17 +0.5 501,065 + ybaL b0478 0.17 -0.6 501,065 + ybaL b0478 0.17 +1.0 501,065 + ybaL b0478 0.17 +0.7 501,065 + ybaL b0478 0.17 -0.7 501,065 + ybaL b0478 0.17 -0.3 501,073 - ybaL b0478 0.17 -1.0 501,073 - ybaL b0478 0.17 +0.6 501,073 - ybaL b0478 0.17 +1.1 501,075 + ybaL b0478 0.17 +1.3 501,178 + ybaL b0478 0.23 +0.4 501,184 - ybaL b0478 0.24 +0.1 501,184 - ybaL b0478 0.24 +0.8 501,316 + ybaL b0478 0.32 +1.7 501,316 + ybaL b0478 0.32 +0.9 501,324 - ybaL b0478 0.32 +0.6 501,358 - ybaL b0478 0.34 -0.0 501,358 - ybaL b0478 0.34 +0.0 501,417 + ybaL b0478 0.38 +0.3 501,417 + ybaL b0478 0.38 -0.2 501,455 + ybaL b0478 0.40 +0.7 501,455 + ybaL b0478 0.40 -0.8 501,468 + ybaL b0478 0.41 +0.5 501,677 + ybaL b0478 0.53 +0.0 501,927 - ybaL b0478 0.68 +0.2 501,986 - ybaL b0478 0.72 +0.1 502,033 - ybaL b0478 0.74 +0.2 502,051 + ybaL b0478 0.75 -0.0 502,105 + ybaL b0478 0.79 -0.1 502,154 + ybaL b0478 0.82 +1.6 502,155 - ybaL b0478 0.82 +0.3 502,155 - ybaL b0478 0.82 +0.3 502,293 - ybaL b0478 0.90 +0.3 502,293 - ybaL b0478 0.90 +0.2 502,346 + -0.2 502,346 + +0.4 502,384 - +0.7 502,384 - +0.6 502,437 - +0.2 502,656 + -0.7 502,816 - +0.3 502,990 - fsr b0479 0.24 -0.1 503,020 + fsr b0479 0.26 +0.4 503,084 + fsr b0479 0.31 +0.3 503,125 - fsr b0479 0.35 +0.3 503,125 - fsr b0479 0.35 +0.6 503,134 + fsr b0479 0.36 +0.2 503,134 + fsr b0479 0.36 -1.1 503,178 - fsr b0479 0.39 +0.1 503,275 - fsr b0479 0.47 -0.1 503,288 - fsr b0479 0.48 -0.5 503,288 - fsr b0479 0.48 -0.6
Or see this region's nucleotide sequence