Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insC and insD-6 overlap by 43 nucleotides insD-6 and yjgX are separated by 92 nucleotides yjgX and yjgZ are separated by 468 nucleotides
b4272: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 4,496,295 to 4,496,660
insC
b4273: insD-6 - KpLE2 phage-like element; IS2 insertion element transposase InsAB' (NCBI), at 4,496,618 to 4,497,523
insD-6
b4575: yjgX - no description, at 4,497,616 to 4,498,814
yjgX
b4277: yjgZ - KpLE2 phage-like element; predicted protein (NCBI), at 4,499,283 to 4,499,612
yjgZ
Position (kb)
4497
4498
4499 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4497.491 kb on + strand at 4497.491 kb on + strand at 4497.492 kb on + strand at 4497.498 kb on + strand at 4497.529 kb on + strand at 4497.579 kb on + strand at 4497.581 kb on + strand at 4497.581 kb on + strand at 4497.589 kb on - strand at 4497.591 kb on + strand at 4497.591 kb on + strand at 4497.591 kb on + strand at 4497.591 kb on + strand at 4497.593 kb on + strand at 4497.593 kb on + strand at 4497.593 kb on + strand at 4497.601 kb on - strand at 4497.633 kb on + strand at 4497.666 kb on - strand at 4497.681 kb on - strand at 4497.710 kb on - strand at 4497.750 kb on + strand, within yjgX at 4497.790 kb on - strand, within yjgX at 4497.790 kb on - strand at 4497.813 kb on + strand, within yjgX at 4497.837 kb on - strand, within yjgX at 4497.837 kb on - strand, within yjgX at 4497.841 kb on - strand, within yjgX at 4497.857 kb on + strand, within yjgX at 4497.857 kb on + strand, within yjgX at 4497.857 kb on - strand, within yjgX at 4497.859 kb on + strand, within yjgX at 4497.860 kb on - strand, within yjgX at 4497.864 kb on - strand, within yjgX at 4497.864 kb on - strand, within yjgX at 4497.864 kb on - strand, within yjgX at 4497.865 kb on - strand, within yjgX at 4497.866 kb on + strand, within yjgX at 4497.871 kb on - strand, within yjgX at 4497.871 kb on - strand, within yjgX at 4497.873 kb on - strand, within yjgX at 4497.878 kb on - strand, within yjgX at 4497.878 kb on - strand, within yjgX at 4497.894 kb on - strand, within yjgX at 4497.894 kb on - strand, within yjgX at 4497.918 kb on + strand, within yjgX at 4497.977 kb on - strand, within yjgX at 4497.977 kb on - strand, within yjgX at 4497.977 kb on - strand, within yjgX at 4497.977 kb on - strand, within yjgX at 4497.977 kb on - strand, within yjgX at 4497.982 kb on - strand, within yjgX at 4498.085 kb on + strand, within yjgX at 4498.085 kb on + strand, within yjgX at 4498.157 kb on - strand, within yjgX at 4498.157 kb on - strand, within yjgX at 4498.187 kb on + strand, within yjgX at 4498.187 kb on + strand, within yjgX at 4498.304 kb on + strand, within yjgX at 4498.319 kb on - strand, within yjgX at 4498.362 kb on + strand, within yjgX at 4498.365 kb on - strand, within yjgX at 4498.388 kb on - strand, within yjgX at 4498.395 kb on - strand, within yjgX at 4498.401 kb on - strand, within yjgX at 4498.403 kb on + strand, within yjgX at 4498.403 kb on + strand, within yjgX at 4498.406 kb on - strand, within yjgX at 4498.406 kb on - strand, within yjgX at 4498.409 kb on - strand, within yjgX at 4498.471 kb on - strand, within yjgX at 4498.473 kb on + strand, within yjgX at 4498.483 kb on + strand, within yjgX at 4498.491 kb on - strand, within yjgX at 4498.491 kb on - strand, within yjgX at 4498.491 kb on - strand, within yjgX at 4498.491 kb on - strand, within yjgX at 4498.491 kb on - strand, within yjgX at 4498.503 kb on - strand, within yjgX at 4498.575 kb on + strand, within yjgX at 4498.588 kb on + strand, within yjgX at 4498.593 kb on - strand, within yjgX at 4498.600 kb on + strand, within yjgX at 4498.600 kb on + strand, within yjgX at 4498.633 kb on + strand, within yjgX at 4498.633 kb on + strand, within yjgX at 4498.648 kb on - strand, within yjgX at 4498.681 kb on - strand, within yjgX at 4498.717 kb on - strand at 4498.855 kb on + strand at 4498.973 kb on - strand at 4498.993 kb on - strand at 4499.122 kb on + strand at 4499.122 kb on + strand at 4499.230 kb on + strand at 4499.238 kb on - strand at 4499.279 kb on - strand at 4499.290 kb on + strand at 4499.290 kb on + strand at 4499.309 kb on - strand at 4499.385 kb on - strand, within yjgZ at 4499.387 kb on + strand, within yjgZ at 4499.408 kb on - strand, within yjgZ at 4499.451 kb on + strand, within yjgZ at 4499.466 kb on - strand, within yjgZ at 4499.486 kb on - strand, within yjgZ at 4499.503 kb on + strand, within yjgZ at 4499.552 kb on - strand, within yjgZ at 4499.584 kb on - strand at 4499.612 kb on + strand at 4499.612 kb on + strand at 4499.641 kb on - strand at 4499.721 kb on - strand at 4499.735 kb on - strand at 4499.735 kb on - strand at 4499.752 kb on - strand at 4499.752 kb on - strand at 4499.770 kb on + strand at 4499.770 kb on + strand at 4499.783 kb on + strand at 4499.793 kb on + strand at 4499.799 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 remove 4,497,491 + -0.9 4,497,491 + +0.1 4,497,492 + -0.0 4,497,498 + +0.4 4,497,529 + -0.0 4,497,579 + -0.5 4,497,581 + -0.6 4,497,581 + -1.4 4,497,589 - -1.3 4,497,591 + +0.5 4,497,591 + -0.4 4,497,591 + -1.2 4,497,591 + -0.1 4,497,593 + -1.5 4,497,593 + -1.5 4,497,593 + -1.6 4,497,601 - +1.8 4,497,633 + +0.5 4,497,666 - +0.7 4,497,681 - +0.4 4,497,710 - -0.1 4,497,750 + yjgX b4575 0.11 -0.0 4,497,790 - yjgX b4575 0.15 -1.0 4,497,790 - -1.4 4,497,813 + yjgX b4575 0.16 +0.4 4,497,837 - yjgX b4575 0.18 -0.7 4,497,837 - yjgX b4575 0.18 -0.2 4,497,841 - yjgX b4575 0.19 -1.3 4,497,857 + yjgX b4575 0.20 -2.1 4,497,857 + yjgX b4575 0.20 -0.2 4,497,857 - yjgX b4575 0.20 -0.2 4,497,859 + yjgX b4575 0.20 -0.3 4,497,860 - yjgX b4575 0.20 -1.0 4,497,864 - yjgX b4575 0.21 -0.1 4,497,864 - yjgX b4575 0.21 -0.0 4,497,864 - yjgX b4575 0.21 -0.2 4,497,865 - yjgX b4575 0.21 -0.4 4,497,866 + yjgX b4575 0.21 +0.5 4,497,871 - yjgX b4575 0.21 -0.1 4,497,871 - yjgX b4575 0.21 -0.1 4,497,873 - yjgX b4575 0.21 -0.9 4,497,878 - yjgX b4575 0.22 +0.2 4,497,878 - yjgX b4575 0.22 -0.3 4,497,894 - yjgX b4575 0.23 -0.8 4,497,894 - yjgX b4575 0.23 -0.0 4,497,918 + yjgX b4575 0.25 +0.1 4,497,977 - yjgX b4575 0.30 +0.0 4,497,977 - yjgX b4575 0.30 -0.4 4,497,977 - yjgX b4575 0.30 -0.3 4,497,977 - yjgX b4575 0.30 -0.9 4,497,977 - yjgX b4575 0.30 +0.7 4,497,982 - yjgX b4575 0.31 -0.9 4,498,085 + yjgX b4575 0.39 -0.0 4,498,085 + yjgX b4575 0.39 -0.1 4,498,157 - yjgX b4575 0.45 -1.8 4,498,157 - yjgX b4575 0.45 +0.7 4,498,187 + yjgX b4575 0.48 +0.9 4,498,187 + yjgX b4575 0.48 -0.1 4,498,304 + yjgX b4575 0.57 -0.4 4,498,319 - yjgX b4575 0.59 -0.2 4,498,362 + yjgX b4575 0.62 -0.3 4,498,365 - yjgX b4575 0.62 -0.6 4,498,388 - yjgX b4575 0.64 -0.2 4,498,395 - yjgX b4575 0.65 -0.9 4,498,401 - yjgX b4575 0.65 +0.5 4,498,403 + yjgX b4575 0.66 +0.3 4,498,403 + yjgX b4575 0.66 +0.0 4,498,406 - yjgX b4575 0.66 +1.3 4,498,406 - yjgX b4575 0.66 -0.3 4,498,409 - yjgX b4575 0.66 -0.8 4,498,471 - yjgX b4575 0.71 -0.8 4,498,473 + yjgX b4575 0.71 -0.0 4,498,483 + yjgX b4575 0.72 -1.1 4,498,491 - yjgX b4575 0.73 -0.4 4,498,491 - yjgX b4575 0.73 +0.2 4,498,491 - yjgX b4575 0.73 +0.1 4,498,491 - yjgX b4575 0.73 +0.1 4,498,491 - yjgX b4575 0.73 -0.2 4,498,503 - yjgX b4575 0.74 -0.0 4,498,575 + yjgX b4575 0.80 -0.7 4,498,588 + yjgX b4575 0.81 +0.6 4,498,593 - yjgX b4575 0.81 +0.3 4,498,600 + yjgX b4575 0.82 -0.6 4,498,600 + yjgX b4575 0.82 -0.3 4,498,633 + yjgX b4575 0.85 -0.4 4,498,633 + yjgX b4575 0.85 -0.5 4,498,648 - yjgX b4575 0.86 -0.2 4,498,681 - yjgX b4575 0.89 -1.1 4,498,717 - -0.0 4,498,855 + +0.3 4,498,973 - +0.4 4,498,993 - -0.6 4,499,122 + -1.1 4,499,122 + -0.1 4,499,230 + +0.4 4,499,238 - -0.9 4,499,279 - -0.5 4,499,290 + -0.6 4,499,290 + -0.9 4,499,309 - -1.6 4,499,385 - yjgZ b4277 0.31 +0.5 4,499,387 + yjgZ b4277 0.32 -0.2 4,499,408 - yjgZ b4277 0.38 +0.1 4,499,451 + yjgZ b4277 0.51 +0.3 4,499,466 - yjgZ b4277 0.55 +0.4 4,499,486 - yjgZ b4277 0.62 +0.0 4,499,503 + yjgZ b4277 0.67 +0.2 4,499,552 - yjgZ b4277 0.82 +0.2 4,499,584 - +0.5 4,499,612 + +0.0 4,499,612 + -0.8 4,499,641 - +0.4 4,499,721 - +0.9 4,499,735 - +0.2 4,499,735 - -0.2 4,499,752 - -0.8 4,499,752 - +0.5 4,499,770 + +0.5 4,499,770 + -0.4 4,499,783 + -0.2 4,499,793 + +0.4 4,499,799 + -0.0
Or see this region's nucleotide sequence