Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mngA and mngB are separated by 17 nucleotides mngB and cydA are separated by 846 nucleotides
b0731: mngA - fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI), at 765,207 to 767,183
mngA
b0732: mngB - alpha-mannosidase (NCBI), at 767,201 to 769,834
mngB
b0733: cydA - cytochrome d terminal oxidase, polypeptide subunit I (VIMSS), at 770,681 to 772,249
cydA
Position (kb) 767
768
769
770 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 766.263 kb on + strand, within mngA at 766.399 kb on - strand, within mngA at 766.767 kb on + strand, within mngA at 766.767 kb on + strand, within mngA at 766.848 kb on + strand, within mngA at 766.848 kb on + strand, within mngA at 767.053 kb on + strand at 767.053 kb on + strand at 767.053 kb on + strand at 767.158 kb on + strand at 767.158 kb on + strand at 767.226 kb on - strand at 767.257 kb on + strand at 767.309 kb on + strand at 767.329 kb on + strand at 767.329 kb on + strand at 767.473 kb on - strand, within mngB at 767.473 kb on - strand, within mngB at 767.473 kb on - strand, within mngB at 767.473 kb on - strand, within mngB at 767.535 kb on - strand, within mngB at 767.614 kb on - strand, within mngB at 767.614 kb on - strand, within mngB at 767.655 kb on + strand, within mngB at 767.717 kb on - strand, within mngB at 767.863 kb on + strand, within mngB at 767.917 kb on + strand, within mngB at 767.917 kb on + strand, within mngB at 767.917 kb on + strand, within mngB at 767.925 kb on - strand, within mngB at 767.937 kb on - strand, within mngB at 767.937 kb on - strand, within mngB at 768.026 kb on + strand, within mngB at 768.124 kb on + strand, within mngB at 768.131 kb on + strand, within mngB at 768.135 kb on - strand, within mngB at 768.135 kb on - strand, within mngB at 768.247 kb on - strand, within mngB at 768.247 kb on - strand, within mngB at 768.343 kb on - strand, within mngB at 768.346 kb on - strand, within mngB at 768.367 kb on + strand, within mngB at 768.367 kb on + strand, within mngB at 768.380 kb on + strand, within mngB at 768.395 kb on - strand, within mngB at 768.454 kb on + strand, within mngB at 768.459 kb on + strand, within mngB at 768.467 kb on - strand, within mngB at 768.483 kb on + strand, within mngB at 768.487 kb on - strand, within mngB at 768.498 kb on + strand, within mngB at 768.498 kb on + strand, within mngB at 768.498 kb on + strand, within mngB at 768.498 kb on + strand, within mngB at 768.498 kb on + strand, within mngB at 768.498 kb on + strand, within mngB at 768.498 kb on + strand, within mngB at 768.503 kb on + strand, within mngB at 768.506 kb on - strand, within mngB at 768.506 kb on - strand, within mngB at 768.506 kb on - strand, within mngB at 768.506 kb on - strand, within mngB at 768.506 kb on - strand, within mngB at 768.527 kb on - strand, within mngB at 768.540 kb on - strand, within mngB at 768.558 kb on + strand, within mngB at 768.558 kb on + strand at 768.561 kb on + strand, within mngB at 768.561 kb on + strand, within mngB at 768.566 kb on - strand, within mngB at 768.573 kb on + strand, within mngB at 768.573 kb on + strand, within mngB at 768.580 kb on + strand, within mngB at 768.586 kb on + strand, within mngB at 768.586 kb on + strand, within mngB at 768.587 kb on + strand, within mngB at 768.592 kb on + strand, within mngB at 768.604 kb on + strand, within mngB at 768.619 kb on + strand, within mngB at 768.620 kb on + strand, within mngB at 768.620 kb on + strand, within mngB at 768.697 kb on + strand, within mngB at 768.703 kb on + strand, within mngB at 768.705 kb on - strand, within mngB at 768.705 kb on - strand, within mngB at 768.708 kb on + strand, within mngB at 768.711 kb on + strand, within mngB at 768.721 kb on - strand, within mngB at 768.721 kb on - strand, within mngB at 768.730 kb on + strand, within mngB at 768.730 kb on + strand, within mngB at 768.731 kb on - strand, within mngB at 768.731 kb on - strand, within mngB at 768.733 kb on - strand, within mngB at 768.738 kb on + strand, within mngB at 768.738 kb on + strand, within mngB at 768.791 kb on + strand, within mngB at 768.791 kb on + strand, within mngB at 768.791 kb on + strand, within mngB at 768.799 kb on - strand, within mngB at 768.799 kb on - strand, within mngB at 768.799 kb on - strand, within mngB at 768.820 kb on + strand, within mngB at 768.820 kb on - strand, within mngB at 768.831 kb on - strand, within mngB at 768.831 kb on - strand, within mngB at 768.831 kb on - strand, within mngB at 768.875 kb on + strand, within mngB at 768.878 kb on - strand, within mngB at 768.890 kb on + strand, within mngB at 768.890 kb on + strand, within mngB at 768.896 kb on - strand, within mngB at 768.898 kb on - strand, within mngB at 768.898 kb on - strand, within mngB at 768.898 kb on - strand, within mngB at 768.901 kb on + strand, within mngB at 768.901 kb on + strand, within mngB at 768.906 kb on - strand, within mngB at 768.911 kb on - strand, within mngB at 768.916 kb on - strand, within mngB at 768.916 kb on - strand, within mngB at 768.936 kb on + strand, within mngB at 768.943 kb on + strand, within mngB at 768.943 kb on + strand, within mngB at 768.957 kb on + strand, within mngB at 768.965 kb on - strand, within mngB at 768.967 kb on + strand, within mngB at 768.989 kb on - strand, within mngB at 769.010 kb on - strand, within mngB at 769.012 kb on - strand, within mngB at 769.032 kb on + strand, within mngB at 769.032 kb on + strand, within mngB at 769.055 kb on + strand, within mngB at 769.071 kb on - strand, within mngB at 769.072 kb on + strand, within mngB at 769.088 kb on - strand, within mngB at 769.091 kb on - strand, within mngB at 769.113 kb on + strand, within mngB at 769.157 kb on - strand, within mngB at 769.233 kb on - strand, within mngB at 769.322 kb on + strand, within mngB at 769.443 kb on - strand, within mngB at 769.451 kb on - strand, within mngB at 769.451 kb on - strand, within mngB at 769.494 kb on + strand, within mngB at 769.533 kb on - strand, within mngB at 769.533 kb on - strand, within mngB at 769.665 kb on - strand at 769.737 kb on + strand at 769.737 kb on + strand at 769.747 kb on + strand at 769.755 kb on - strand at 769.771 kb on - strand at 769.812 kb on - strand at 769.855 kb on + strand at 769.874 kb on + strand at 769.895 kb on - strand at 769.895 kb on - strand at 769.927 kb on - strand at 769.947 kb on + strand at 769.954 kb on + strand at 769.964 kb on + strand at 769.964 kb on + strand at 769.966 kb on + strand at 769.971 kb on + strand at 769.972 kb on - strand at 769.972 kb on - strand at 769.974 kb on + strand at 769.976 kb on + strand at 769.976 kb on + strand at 769.976 kb on + strand at 769.980 kb on + strand at 769.980 kb on + strand at 769.981 kb on + strand at 769.992 kb on - strand at 770.009 kb on + strand at 770.016 kb on + strand at 770.034 kb on - strand at 770.045 kb on - strand at 770.045 kb on - strand at 770.053 kb on + strand at 770.132 kb on + strand at 770.144 kb on + strand at 770.144 kb on + strand at 770.151 kb on - strand at 770.153 kb on - strand at 770.160 kb on + strand at 770.160 kb on + strand at 770.168 kb on - strand at 770.198 kb on - strand at 770.203 kb on + strand at 770.206 kb on - strand at 770.257 kb on + strand at 770.277 kb on - strand at 770.310 kb on - strand at 770.310 kb on - strand at 770.322 kb on - strand at 770.322 kb on - strand at 770.346 kb on + strand at 770.346 kb on + strand at 770.354 kb on - strand at 770.354 kb on - strand at 770.354 kb on - strand at 770.356 kb on - strand at 770.356 kb on - strand at 770.402 kb on - strand at 770.402 kb on - strand at 770.437 kb on + strand at 770.582 kb on + strand at 770.582 kb on + strand at 770.619 kb on + strand at 770.625 kb on + strand at 770.661 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 remove 766,263 + mngA b0731 0.53 -0.3 766,399 - mngA b0731 0.60 -0.1 766,767 + mngA b0731 0.79 +0.3 766,767 + mngA b0731 0.79 +0.5 766,848 + mngA b0731 0.83 -1.0 766,848 + mngA b0731 0.83 -0.2 767,053 + -0.5 767,053 + -0.3 767,053 + -0.7 767,158 + -0.6 767,158 + -1.5 767,226 - +0.4 767,257 + -0.8 767,309 + -0.4 767,329 + -0.2 767,329 + +0.2 767,473 - mngB b0732 0.10 -0.1 767,473 - mngB b0732 0.10 -0.4 767,473 - mngB b0732 0.10 +0.2 767,473 - mngB b0732 0.10 -0.5 767,535 - mngB b0732 0.13 -0.4 767,614 - mngB b0732 0.16 -0.1 767,614 - mngB b0732 0.16 -0.3 767,655 + mngB b0732 0.17 +0.2 767,717 - mngB b0732 0.20 -0.0 767,863 + mngB b0732 0.25 -0.6 767,917 + mngB b0732 0.27 -0.4 767,917 + mngB b0732 0.27 -0.8 767,917 + mngB b0732 0.27 +0.1 767,925 - mngB b0732 0.27 -0.1 767,937 - mngB b0732 0.28 -0.4 767,937 - mngB b0732 0.28 -0.4 768,026 + mngB b0732 0.31 -1.8 768,124 + mngB b0732 0.35 +0.3 768,131 + mngB b0732 0.35 -0.4 768,135 - mngB b0732 0.35 -0.1 768,135 - mngB b0732 0.35 +0.2 768,247 - mngB b0732 0.40 +0.5 768,247 - mngB b0732 0.40 +1.0 768,343 - mngB b0732 0.43 +0.2 768,346 - mngB b0732 0.43 +1.1 768,367 + mngB b0732 0.44 +0.0 768,367 + mngB b0732 0.44 -0.3 768,380 + mngB b0732 0.45 -0.3 768,395 - mngB b0732 0.45 -0.5 768,454 + mngB b0732 0.48 -0.3 768,459 + mngB b0732 0.48 +0.4 768,467 - mngB b0732 0.48 -0.7 768,483 + mngB b0732 0.49 +0.2 768,487 - mngB b0732 0.49 +0.8 768,498 + mngB b0732 0.49 -0.8 768,498 + mngB b0732 0.49 -1.3 768,498 + mngB b0732 0.49 -0.3 768,498 + mngB b0732 0.49 -0.5 768,498 + mngB b0732 0.49 -0.1 768,498 + mngB b0732 0.49 -0.1 768,498 + mngB b0732 0.49 +0.0 768,503 + mngB b0732 0.49 -1.6 768,506 - mngB b0732 0.50 -0.0 768,506 - mngB b0732 0.50 +0.0 768,506 - mngB b0732 0.50 +1.8 768,506 - mngB b0732 0.50 +1.0 768,506 - mngB b0732 0.50 -0.5 768,527 - mngB b0732 0.50 +0.2 768,540 - mngB b0732 0.51 +0.5 768,558 + mngB b0732 0.52 +0.3 768,558 + +0.1 768,561 + mngB b0732 0.52 -0.1 768,561 + mngB b0732 0.52 +0.3 768,566 - mngB b0732 0.52 +0.1 768,573 + mngB b0732 0.52 +0.1 768,573 + mngB b0732 0.52 -0.3 768,580 + mngB b0732 0.52 -0.5 768,586 + mngB b0732 0.53 +1.0 768,586 + mngB b0732 0.53 -0.7 768,587 + mngB b0732 0.53 -0.1 768,592 + mngB b0732 0.53 -0.1 768,604 + mngB b0732 0.53 -0.2 768,619 + mngB b0732 0.54 +0.3 768,620 + mngB b0732 0.54 -0.4 768,620 + mngB b0732 0.54 +0.3 768,697 + mngB b0732 0.57 +0.2 768,703 + mngB b0732 0.57 -0.9 768,705 - mngB b0732 0.57 +0.3 768,705 - mngB b0732 0.57 -0.7 768,708 + mngB b0732 0.57 +0.2 768,711 + mngB b0732 0.57 +1.6 768,721 - mngB b0732 0.58 +1.2 768,721 - mngB b0732 0.58 -1.7 768,730 + mngB b0732 0.58 +0.4 768,730 + mngB b0732 0.58 -0.3 768,731 - mngB b0732 0.58 +0.3 768,731 - mngB b0732 0.58 -0.7 768,733 - mngB b0732 0.58 +0.6 768,738 + mngB b0732 0.58 -0.1 768,738 + mngB b0732 0.58 -0.6 768,791 + mngB b0732 0.60 -0.0 768,791 + mngB b0732 0.60 -0.6 768,791 + mngB b0732 0.60 +0.1 768,799 - mngB b0732 0.61 +0.8 768,799 - mngB b0732 0.61 +0.4 768,799 - mngB b0732 0.61 +0.1 768,820 + mngB b0732 0.61 -0.4 768,820 - mngB b0732 0.61 -0.6 768,831 - mngB b0732 0.62 -1.0 768,831 - mngB b0732 0.62 +1.0 768,831 - mngB b0732 0.62 -0.7 768,875 + mngB b0732 0.64 +0.1 768,878 - mngB b0732 0.64 -1.1 768,890 + mngB b0732 0.64 +0.4 768,890 + mngB b0732 0.64 +1.0 768,896 - mngB b0732 0.64 -0.2 768,898 - mngB b0732 0.64 +1.5 768,898 - mngB b0732 0.64 +0.2 768,898 - mngB b0732 0.64 -1.4 768,901 + mngB b0732 0.65 +1.1 768,901 + mngB b0732 0.65 +0.3 768,906 - mngB b0732 0.65 -0.1 768,911 - mngB b0732 0.65 -0.7 768,916 - mngB b0732 0.65 +0.6 768,916 - mngB b0732 0.65 -0.4 768,936 + mngB b0732 0.66 -0.4 768,943 + mngB b0732 0.66 -0.1 768,943 + mngB b0732 0.66 +0.6 768,957 + mngB b0732 0.67 +0.1 768,965 - mngB b0732 0.67 +0.4 768,967 + mngB b0732 0.67 -0.6 768,989 - mngB b0732 0.68 +0.1 769,010 - mngB b0732 0.69 +0.5 769,012 - mngB b0732 0.69 +0.0 769,032 + mngB b0732 0.70 +0.5 769,032 + mngB b0732 0.70 -0.4 769,055 + mngB b0732 0.70 -0.1 769,071 - mngB b0732 0.71 -0.2 769,072 + mngB b0732 0.71 -0.2 769,088 - mngB b0732 0.72 +0.5 769,091 - mngB b0732 0.72 -0.1 769,113 + mngB b0732 0.73 -0.2 769,157 - mngB b0732 0.74 -0.2 769,233 - mngB b0732 0.77 -0.2 769,322 + mngB b0732 0.81 -0.7 769,443 - mngB b0732 0.85 +0.6 769,451 - mngB b0732 0.85 -1.3 769,451 - mngB b0732 0.85 +1.4 769,494 + mngB b0732 0.87 -0.6 769,533 - mngB b0732 0.89 +0.9 769,533 - mngB b0732 0.89 -0.4 769,665 - +0.4 769,737 + +0.2 769,737 + -0.8 769,747 + +0.3 769,755 - +1.2 769,771 - +1.1 769,812 - +0.0 769,855 + -0.3 769,874 + -0.7 769,895 - +0.4 769,895 - +0.4 769,927 - +2.0 769,947 + +0.5 769,954 + +0.1 769,964 + -0.3 769,964 + +1.2 769,966 + +0.3 769,971 + +0.3 769,972 - -0.1 769,972 - +1.7 769,974 + +0.2 769,976 + +0.5 769,976 + +0.4 769,976 + +0.5 769,980 + -0.1 769,980 + +0.3 769,981 + -1.4 769,992 - -1.3 770,009 + +0.3 770,016 + +0.4 770,034 - +0.6 770,045 - +1.8 770,045 - -0.7 770,053 + -0.5 770,132 + -2.1 770,144 + +0.3 770,144 + -0.6 770,151 - +0.2 770,153 - +0.2 770,160 + -0.7 770,160 + +0.1 770,168 - +0.8 770,198 - +1.4 770,203 + +0.1 770,206 - +0.6 770,257 + -0.6 770,277 - +2.0 770,310 - +1.9 770,310 - +0.8 770,322 - +1.4 770,322 - +0.2 770,346 + +0.1 770,346 + +0.3 770,354 - -0.0 770,354 - +1.5 770,354 - +1.8 770,356 - +4.0 770,356 - -0.1 770,402 - +1.1 770,402 - +1.0 770,437 + +0.3 770,582 + +0.4 770,582 + -0.3 770,619 + +3.7 770,625 + +0.3 770,661 + +0.9
Or see this region's nucleotide sequence