Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpC and prpD are separated by 33 nucleotides prpD and prpE are separated by 39 nucleotides
b0333: prpC - 2-methylcitrate synthase (NCBI), at 349,236 to 350,405
prpC
b0334: prpD - 2-methylcitrate dehydratase (NCBI), at 350,439 to 351,890
prpD
b0335: prpE - predicted propionyl-CoA synthetase with ATPase domain (NCBI), at 351,930 to 353,816
prpE
Position (kb) 350
351
352 Strain fitness (log2 ratio) -1
0
1 at 349.502 kb on + strand, within prpC at 349.502 kb on + strand, within prpC at 349.642 kb on - strand, within prpC at 349.691 kb on + strand, within prpC at 349.691 kb on + strand, within prpC at 349.744 kb on - strand, within prpC at 349.797 kb on - strand, within prpC at 349.895 kb on - strand, within prpC at 350.020 kb on - strand, within prpC at 350.068 kb on - strand, within prpC at 350.069 kb on - strand, within prpC at 350.069 kb on - strand, within prpC at 350.142 kb on + strand, within prpC at 350.187 kb on + strand, within prpC at 350.327 kb on - strand at 350.327 kb on - strand at 350.395 kb on + strand at 350.398 kb on - strand at 350.550 kb on - strand at 350.550 kb on - strand at 350.626 kb on - strand, within prpD at 350.639 kb on - strand, within prpD at 350.639 kb on - strand, within prpD at 350.771 kb on - strand, within prpD at 351.073 kb on + strand, within prpD at 351.080 kb on + strand, within prpD at 351.080 kb on + strand, within prpD at 351.236 kb on + strand, within prpD at 351.419 kb on - strand, within prpD at 351.481 kb on - strand, within prpD at 351.565 kb on + strand, within prpD at 351.573 kb on - strand, within prpD at 351.691 kb on + strand, within prpD at 351.691 kb on + strand, within prpD at 351.702 kb on + strand, within prpD at 351.707 kb on - strand, within prpD at 351.918 kb on - strand at 351.918 kb on - strand at 351.977 kb on + strand at 351.977 kb on + strand at 351.977 kb on + strand at 352.027 kb on - strand at 352.027 kb on - strand at 352.185 kb on - strand, within prpE at 352.242 kb on + strand, within prpE at 352.242 kb on + strand, within prpE at 352.626 kb on + strand, within prpE at 352.648 kb on + strand, within prpE at 352.753 kb on + strand, within prpE at 352.783 kb on + strand, within prpE at 352.813 kb on - strand, within prpE at 352.813 kb on - strand, within prpE at 352.871 kb on + strand, within prpE
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 remove 349,502 + prpC b0333 0.23 +0.2 349,502 + prpC b0333 0.23 +0.7 349,642 - prpC b0333 0.35 -0.1 349,691 + prpC b0333 0.39 +0.9 349,691 + prpC b0333 0.39 -0.1 349,744 - prpC b0333 0.43 -0.7 349,797 - prpC b0333 0.48 -0.5 349,895 - prpC b0333 0.56 -0.8 350,020 - prpC b0333 0.67 -0.0 350,068 - prpC b0333 0.71 +0.0 350,069 - prpC b0333 0.71 +0.4 350,069 - prpC b0333 0.71 -0.1 350,142 + prpC b0333 0.77 +0.3 350,187 + prpC b0333 0.81 -0.1 350,327 - -0.5 350,327 - +0.9 350,395 + +0.4 350,398 - -1.1 350,550 - -0.7 350,550 - +0.5 350,626 - prpD b0334 0.13 -0.2 350,639 - prpD b0334 0.14 -0.1 350,639 - prpD b0334 0.14 -0.2 350,771 - prpD b0334 0.23 -0.4 351,073 + prpD b0334 0.44 -0.2 351,080 + prpD b0334 0.44 -0.2 351,080 + prpD b0334 0.44 -0.4 351,236 + prpD b0334 0.55 -0.9 351,419 - prpD b0334 0.67 +0.0 351,481 - prpD b0334 0.72 -0.5 351,565 + prpD b0334 0.78 -0.4 351,573 - prpD b0334 0.78 +0.5 351,691 + prpD b0334 0.86 -0.5 351,691 + prpD b0334 0.86 -0.4 351,702 + prpD b0334 0.87 -0.8 351,707 - prpD b0334 0.87 -0.6 351,918 - -0.1 351,918 - -1.3 351,977 + +0.3 351,977 + -0.5 351,977 + -0.6 352,027 - -1.0 352,027 - -0.7 352,185 - prpE b0335 0.14 +0.2 352,242 + prpE b0335 0.17 +0.2 352,242 + prpE b0335 0.17 -0.6 352,626 + prpE b0335 0.37 +0.3 352,648 + prpE b0335 0.38 -0.1 352,753 + prpE b0335 0.44 -1.3 352,783 + prpE b0335 0.45 -0.6 352,813 - prpE b0335 0.47 -0.3 352,813 - prpE b0335 0.47 +0.3 352,871 + prpE b0335 0.50 +0.1
Or see this region's nucleotide sequence