Strain Fitness in Escherichia coli BW25113 around b4254

Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Outgrowth from conjugation with pRH23 in LB+Gent50
4,474,400	-	yjgL	b4253	0.52	-0.0
4,474,456	+	yjgL	b4253	0.55	+1.6
4,475,127	+				+1.6
4,475,137	+				+1.6
4,475,165	+				+1.6
4,475,175	+				+0.0
4,475,234	-				-0.1
4,475,235	+				-0.4
4,475,250	+				-2.9
4,475,267	-				+1.6
4,475,277	-				-0.7
4,475,357	-				+0.6
4,475,357	-				-0.3
4,475,361	-				+0.2
4,475,361	-				+0.0
4,475,365	-				+0.3
4,475,367	-				-0.2
4,475,367	-				-0.1
4,475,381	-				-0.2
4,475,381	-				+0.2
4,475,381	-				+0.4
4,475,414	+				-0.6
4,475,422	-				-0.2
4,475,422	-				+0.5
4,475,434	+	argI	b4254	0.10	-0.4
4,475,434	+	argI	b4254	0.10	-0.0
4,475,434	+	argI	b4254	0.10	+1.6
4,475,434	+	argI	b4254	0.10	+0.5
4,475,435	-	argI	b4254	0.10	-0.5
4,475,437	-	argI	b4254	0.11	-0.4
4,475,437	-	argI	b4254	0.11	-0.5
4,475,442	-	argI	b4254	0.11	-0.3
4,475,442	-	argI	b4254	0.11	+2.0
4,475,442	-	argI	b4254	0.11	+0.1
4,475,442	-	argI	b4254	0.11	-0.3
4,475,442	-	argI	b4254	0.11	+1.3
4,475,445	-	argI	b4254	0.11	+0.5
4,475,445	-	argI	b4254	0.11	-0.2
4,475,455	-	argI	b4254	0.12	+0.1
4,475,455	-	argI	b4254	0.12	+0.4
4,475,490	-	argI	b4254	0.16	+0.2
4,475,545	-	argI	b4254	0.21	+0.1
4,475,567	-	argI	b4254	0.24	+0.0
4,475,632	+	argI	b4254	0.30	+0.2
4,475,670	-	argI	b4254	0.34	+1.6
4,475,716	+	argI	b4254	0.38	-0.6
4,475,840	-	argI	b4254	0.51	+0.4
4,475,850	+	argI	b4254	0.52	-0.1
4,475,850	+	argI	b4254	0.52	-0.9
4,475,911	+	argI	b4254	0.58	-0.3
4,475,911	+	argI	b4254	0.58	-0.1
4,475,963	-	argI	b4254	0.63	-0.3
4,476,025	-	argI	b4254	0.69	-0.1
4,476,061	+	argI	b4254	0.73	+0.0
4,476,061	+	argI	b4254	0.73	+0.5
4,476,069	-	argI	b4254	0.74	+0.3
4,476,069	-	argI	b4254	0.74	+0.3
4,476,104	+	argI	b4254	0.77	-0.8
4,476,104	+	argI	b4254	0.77	+0.7
4,476,142	+	argI	b4254	0.81	+1.2
4,476,165	-	argI	b4254	0.83	+0.1
4,476,272	+				+0.1
4,476,272	+				-0.3
4,476,330	+				+0.2
4,476,339	-				+0.4
4,476,442	+				-0.3
4,476,466	-				+0.3
4,476,585	+	yjgD	b4255	0.21	-0.3
4,476,588	+	yjgD	b4255	0.22	-0.1
4,476,588	+	yjgD	b4255	0.22	+0.2
4,476,588	+	yjgD	b4255	0.22	-0.8
4,476,663	+	yjgD	b4255	0.40	+0.3
4,476,673	-	yjgD	b4255	0.42	+0.2
4,476,680	+	yjgD	b4255	0.44	-0.4
4,476,680	+	yjgD	b4255	0.44	+0.5
4,476,741	-	yjgD	b4255	0.59	+0.8
4,476,822	-	yjgD	b4255	0.78	-0.6
4,476,930	+				-0.1
4,476,969	+				-0.2
4,477,018	+				+0.5
4,477,042	-				-0.5
4,477,072	+				+0.0
4,477,072	+				-1.3
4,477,090	-				-0.4
4,477,090	-				-0.1
4,477,108	-	yjgM	b4256	0.10	-1.7
4,477,108	-	yjgM	b4256	0.10	-0.7
4,477,119	+	yjgM	b4256	0.12	+0.6
4,477,119	+	yjgM	b4256	0.12	+0.4
4,477,130	+	yjgM	b4256	0.14	-0.4
4,477,130	+	yjgM	b4256	0.14	-0.9
4,477,184	+	yjgM	b4256	0.25	-0.3
4,477,192	+	yjgM	b4256	0.27	+0.1
4,477,202	+	yjgM	b4256	0.29	-0.7
4,477,202	-	yjgM	b4256	0.29	+0.2
4,477,213	+	yjgM	b4256	0.31	-1.7
4,477,213	-	yjgM	b4256	0.31	+0.2
4,477,219	+	yjgM	b4256	0.32	-0.1
4,477,310	-	yjgM	b4256	0.50	-0.3
4,477,319	+	yjgM	b4256	0.52	-0.0
4,477,331	+	yjgM	b4256	0.54	-0.0