Strain Fitness in Escherichia coli BW25113 around b3075

Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Outgrowth from conjugation with pRH23 in LB+Gent50
3,218,494	+	ygjG	b3073	0.71	+0.4
3,218,532	-	ygjG	b3073	0.74	+0.2
3,218,591	-	ygjG	b3073	0.78	+0.1
3,218,591	-	ygjG	b3073	0.78	-0.0
3,218,663	-	ygjG	b3073	0.83	-0.4
3,218,663	-	ygjG	b3073	0.83	+0.3
3,218,826	+				+0.1
3,218,833	-				+0.1
3,218,855	+				-0.3
3,218,959	+				-0.7
3,218,992	+	ygjH	b3074	0.17	-0.8
3,218,992	+	ygjH	b3074	0.17	-0.4
3,219,018	+	ygjH	b3074	0.24	-0.5
3,219,018	+	ygjH	b3074	0.24	-0.4
3,219,077	-	ygjH	b3074	0.42	+0.8
3,219,077	-	ygjH	b3074	0.42	-0.3
3,219,077	-	ygjH	b3074	0.42	+0.5
3,219,117	-	ygjH	b3074	0.54	-0.3
3,219,119	+	ygjH	b3074	0.55	+0.0
3,219,384	-				-2.9
3,219,384	-				+0.4
3,219,431	-				-0.6
3,219,462	+				-0.3
3,219,549	-				-2.3
3,219,549	-				-2.9
3,219,628	-	ebgR	b3075	0.14	-2.3
3,219,628	-	ebgR	b3075	0.14	-1.9
3,219,650	-	ebgR	b3075	0.16	-2.6
3,219,689	+	ebgR	b3075	0.20	-2.0
3,219,691	+	ebgR	b3075	0.21	-4.1
3,219,787	+	ebgR	b3075	0.30	-0.8
3,219,803	-	ebgR	b3075	0.32	-3.2
3,219,818	-	ebgR	b3075	0.34	-1.7
3,219,861	+	ebgR	b3075	0.38	-1.9
3,220,087	-	ebgR	b3075	0.61	-2.3
3,220,192	-	ebgR	b3075	0.72	+0.3
3,220,237	-	ebgR	b3075	0.76	-2.7
3,220,237	-	ebgR	b3075	0.76	-0.5
3,220,274	-	ebgR	b3075	0.80	-2.3
3,220,274	-	ebgR	b3075	0.80	-1.4
3,220,392	-				-0.5
3,220,546	-				+0.2
3,220,546	-				+0.3
3,220,728	+				-0.0
3,220,889	+				+0.0
3,220,889	+				-0.3
3,220,971	+	ebgA	b3076	0.10	-0.7
3,220,979	-	ebgA	b3076	0.10	-0.8
3,221,075	+	ebgA	b3076	0.14	+0.1
3,221,092	+	ebgA	b3076	0.14	+0.0
3,221,092	+	ebgA	b3076	0.14	-0.3
3,221,092	+	ebgA	b3076	0.14	-0.4
3,221,100	-	ebgA	b3076	0.14	+0.7
3,221,100	-	ebgA	b3076	0.14	-0.2
3,221,110	+	ebgA	b3076	0.15	+0.2
3,221,167	-	ebgA	b3076	0.17	-0.2
3,221,198	+				-0.3
3,221,198	+	ebgA	b3076	0.18	-0.3
3,221,216	+	ebgA	b3076	0.18	+0.2
3,221,216	+	ebgA	b3076	0.18	+0.6
3,221,329	-	ebgA	b3076	0.22	-0.4
3,221,390	+	ebgA	b3076	0.24	-0.2
3,221,390	+	ebgA	b3076	0.24	+0.7
3,221,397	+	ebgA	b3076	0.24	+0.5
3,221,426	+	ebgA	b3076	0.25	-0.2
3,221,434	-	ebgA	b3076	0.25	-1.5