Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nupG and speC are separated by 49 nucleotides speC and yqgA are separated by 397 nucleotides
b2964: nupG - transport of nucleosides, permease protein (VIMSS), at 3,103,736 to 3,104,992
nupG
b2965: speC - ornithine decarboxylase isozyme (VIMSS), at 3,105,042 to 3,107,177
speC
b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282
yqgA
Position (kb)
3105
3106
3107
3108 Strain fitness (log2 ratio)
-2
-1
0
1 at 3104.061 kb on + strand, within nupG at 3104.082 kb on + strand, within nupG at 3104.091 kb on - strand, within nupG at 3104.152 kb on - strand, within nupG at 3104.152 kb on - strand, within nupG at 3104.172 kb on - strand, within nupG at 3104.179 kb on - strand, within nupG at 3104.179 kb on - strand, within nupG at 3104.211 kb on + strand, within nupG at 3104.211 kb on + strand, within nupG at 3104.232 kb on - strand, within nupG at 3104.232 kb on - strand, within nupG at 3104.234 kb on + strand, within nupG at 3104.234 kb on + strand, within nupG at 3104.321 kb on + strand, within nupG at 3104.326 kb on - strand, within nupG at 3104.443 kb on - strand, within nupG at 3104.541 kb on + strand, within nupG at 3104.603 kb on - strand, within nupG at 3104.650 kb on + strand, within nupG at 3104.655 kb on + strand, within nupG at 3104.663 kb on - strand, within nupG at 3104.692 kb on - strand, within nupG at 3104.697 kb on - strand, within nupG at 3104.697 kb on - strand, within nupG at 3104.707 kb on - strand, within nupG at 3104.813 kb on - strand, within nupG at 3104.911 kb on + strand at 3104.911 kb on + strand at 3104.912 kb on + strand at 3104.912 kb on + strand at 3104.912 kb on + strand at 3104.915 kb on - strand at 3105.094 kb on + strand at 3105.173 kb on - strand at 3105.200 kb on - strand at 3105.287 kb on - strand, within speC at 3105.322 kb on - strand, within speC at 3105.322 kb on - strand, within speC at 3105.396 kb on - strand, within speC at 3105.400 kb on - strand, within speC at 3105.430 kb on + strand, within speC at 3105.430 kb on + strand, within speC at 3105.436 kb on - strand, within speC at 3105.636 kb on - strand, within speC at 3105.646 kb on + strand, within speC at 3105.647 kb on - strand, within speC at 3105.705 kb on + strand, within speC at 3105.730 kb on + strand, within speC at 3105.730 kb on + strand, within speC at 3105.746 kb on + strand, within speC at 3105.746 kb on + strand, within speC at 3105.888 kb on - strand, within speC at 3105.911 kb on + strand, within speC at 3105.926 kb on - strand, within speC at 3105.929 kb on - strand, within speC at 3105.929 kb on - strand, within speC at 3105.945 kb on - strand, within speC at 3106.012 kb on + strand, within speC at 3106.017 kb on + strand, within speC at 3106.017 kb on + strand, within speC at 3106.031 kb on + strand, within speC at 3106.039 kb on - strand, within speC at 3106.112 kb on - strand, within speC at 3106.136 kb on - strand, within speC at 3106.136 kb on - strand, within speC at 3106.257 kb on - strand, within speC at 3106.266 kb on + strand, within speC at 3106.305 kb on + strand, within speC at 3106.305 kb on + strand, within speC at 3106.310 kb on + strand, within speC at 3106.310 kb on + strand, within speC at 3106.313 kb on - strand, within speC at 3106.318 kb on - strand, within speC at 3106.318 kb on - strand, within speC at 3106.318 kb on - strand, within speC at 3106.318 kb on - strand, within speC at 3106.351 kb on - strand, within speC at 3106.413 kb on - strand, within speC at 3106.464 kb on - strand, within speC at 3106.564 kb on + strand, within speC at 3106.727 kb on - strand, within speC at 3106.732 kb on - strand, within speC at 3106.811 kb on + strand, within speC at 3106.811 kb on - strand, within speC at 3106.825 kb on + strand, within speC at 3106.825 kb on + strand, within speC at 3106.841 kb on + strand, within speC at 3106.856 kb on - strand, within speC at 3106.856 kb on - strand, within speC at 3107.101 kb on + strand at 3107.101 kb on + strand at 3107.147 kb on - strand at 3107.246 kb on + strand at 3107.246 kb on + strand at 3107.401 kb on + strand at 3107.410 kb on - strand at 3107.496 kb on - strand at 3107.496 kb on - strand at 3107.522 kb on + strand at 3107.580 kb on - strand at 3107.608 kb on + strand at 3107.611 kb on + strand at 3107.611 kb on + strand at 3107.616 kb on - strand at 3107.895 kb on + strand, within yqgA at 3107.918 kb on - strand, within yqgA at 3107.932 kb on - strand, within yqgA at 3108.033 kb on + strand, within yqgA at 3108.117 kb on + strand, within yqgA at 3108.160 kb on + strand, within yqgA
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 3,104,061 + nupG b2964 0.26 +0.3 3,104,082 + nupG b2964 0.28 -1.1 3,104,091 - nupG b2964 0.28 -0.2 3,104,152 - nupG b2964 0.33 -0.4 3,104,152 - nupG b2964 0.33 +0.4 3,104,172 - nupG b2964 0.35 -0.1 3,104,179 - nupG b2964 0.35 -0.1 3,104,179 - nupG b2964 0.35 -0.3 3,104,211 + nupG b2964 0.38 +1.1 3,104,211 + nupG b2964 0.38 -0.6 3,104,232 - nupG b2964 0.39 +0.4 3,104,232 - nupG b2964 0.39 -0.0 3,104,234 + nupG b2964 0.40 +0.6 3,104,234 + nupG b2964 0.40 +0.4 3,104,321 + nupG b2964 0.47 -1.6 3,104,326 - nupG b2964 0.47 +0.1 3,104,443 - nupG b2964 0.56 +0.3 3,104,541 + nupG b2964 0.64 -0.2 3,104,603 - nupG b2964 0.69 -0.6 3,104,650 + nupG b2964 0.73 -0.1 3,104,655 + nupG b2964 0.73 +0.5 3,104,663 - nupG b2964 0.74 +0.0 3,104,692 - nupG b2964 0.76 -0.3 3,104,697 - nupG b2964 0.76 -0.7 3,104,697 - nupG b2964 0.76 -0.4 3,104,707 - nupG b2964 0.77 -0.8 3,104,813 - nupG b2964 0.86 -0.8 3,104,911 + -0.3 3,104,911 + +0.3 3,104,912 + +0.0 3,104,912 + -0.6 3,104,912 + +0.1 3,104,915 - -0.3 3,105,094 + +0.4 3,105,173 - +0.5 3,105,200 - -0.1 3,105,287 - speC b2965 0.11 -0.3 3,105,322 - speC b2965 0.13 +1.0 3,105,322 - speC b2965 0.13 -0.2 3,105,396 - speC b2965 0.17 +0.3 3,105,400 - speC b2965 0.17 +1.2 3,105,430 + speC b2965 0.18 +0.5 3,105,430 + speC b2965 0.18 +0.3 3,105,436 - speC b2965 0.18 +0.1 3,105,636 - speC b2965 0.28 +0.7 3,105,646 + speC b2965 0.28 +0.2 3,105,647 - speC b2965 0.28 +0.1 3,105,705 + speC b2965 0.31 +0.7 3,105,730 + speC b2965 0.32 -0.0 3,105,730 + speC b2965 0.32 -0.3 3,105,746 + speC b2965 0.33 -1.8 3,105,746 + speC b2965 0.33 -0.1 3,105,888 - speC b2965 0.40 -0.2 3,105,911 + speC b2965 0.41 +0.1 3,105,926 - speC b2965 0.41 +0.2 3,105,929 - speC b2965 0.42 -0.7 3,105,929 - speC b2965 0.42 -0.2 3,105,945 - speC b2965 0.42 -0.2 3,106,012 + speC b2965 0.45 -0.6 3,106,017 + speC b2965 0.46 +0.3 3,106,017 + speC b2965 0.46 +0.2 3,106,031 + speC b2965 0.46 +0.3 3,106,039 - speC b2965 0.47 -0.1 3,106,112 - speC b2965 0.50 -0.6 3,106,136 - speC b2965 0.51 -1.0 3,106,136 - speC b2965 0.51 +0.4 3,106,257 - speC b2965 0.57 +0.4 3,106,266 + speC b2965 0.57 -0.2 3,106,305 + speC b2965 0.59 -0.2 3,106,305 + speC b2965 0.59 +0.6 3,106,310 + speC b2965 0.59 -0.6 3,106,310 + speC b2965 0.59 +0.7 3,106,313 - speC b2965 0.60 -0.6 3,106,318 - speC b2965 0.60 +1.5 3,106,318 - speC b2965 0.60 +0.3 3,106,318 - speC b2965 0.60 +0.5 3,106,318 - speC b2965 0.60 +1.5 3,106,351 - speC b2965 0.61 -0.0 3,106,413 - speC b2965 0.64 -1.2 3,106,464 - speC b2965 0.67 -0.6 3,106,564 + speC b2965 0.71 +0.1 3,106,727 - speC b2965 0.79 -0.5 3,106,732 - speC b2965 0.79 -0.6 3,106,811 + speC b2965 0.83 -0.1 3,106,811 - speC b2965 0.83 +0.3 3,106,825 + speC b2965 0.83 +0.2 3,106,825 + speC b2965 0.83 +1.0 3,106,841 + speC b2965 0.84 -0.2 3,106,856 - speC b2965 0.85 +0.4 3,106,856 - speC b2965 0.85 +0.0 3,107,101 + +0.3 3,107,101 + +0.4 3,107,147 - -0.4 3,107,246 + +0.3 3,107,246 + +0.5 3,107,401 + -0.4 3,107,410 - -0.1 3,107,496 - +0.2 3,107,496 - +0.3 3,107,522 + +0.1 3,107,580 - -0.4 3,107,608 + +0.2 3,107,611 + -0.2 3,107,611 + -0.1 3,107,616 - -0.6 3,107,895 + yqgA b2966 0.45 -0.5 3,107,918 - yqgA b2966 0.48 -0.1 3,107,932 - yqgA b2966 0.50 +0.1 3,108,033 + yqgA b2966 0.65 -0.6 3,108,117 + yqgA b2966 0.77 +0.2 3,108,160 + yqgA b2966 0.83 -0.2
Or see this region's nucleotide sequence