Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1758 and nudG are separated by 86 nucleotides nudG and ynjH overlap by 35 nucleotides ynjH and gdhA are separated by 235 nucleotides gdhA and b1762 are separated by 116 nucleotides
b1758: b1758 - putative cytochrome oxidase (VIMSS), at 1,838,807 to 1,839,427
b1758
b1759: nudG - pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase (NCBI), at 1,839,514 to 1,839,921
nudG
b1760: ynjH - hypothetical protein (NCBI), at 1,839,887 to 1,840,159
ynjH
b1761: gdhA - glutamate dehydrogenase (NCBI), at 1,840,395 to 1,841,738
gdhA
b1762: b1762 - orf, hypothetical protein (VIMSS), at 1,841,855 to 1,842,895
b1762
Position (kb)
1840
1841
1842 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1839.552 kb on - strand at 1839.552 kb on - strand at 1839.760 kb on - strand, within nudG at 1839.893 kb on - strand at 1840.011 kb on + strand, within ynjH at 1840.026 kb on - strand, within ynjH at 1840.029 kb on - strand, within ynjH at 1840.031 kb on - strand, within ynjH at 1840.258 kb on - strand at 1840.325 kb on - strand at 1840.430 kb on - strand at 1840.876 kb on - strand, within gdhA at 1840.886 kb on + strand, within gdhA at 1840.886 kb on + strand, within gdhA at 1840.971 kb on + strand, within gdhA at 1840.971 kb on + strand, within gdhA at 1841.012 kb on - strand, within gdhA at 1841.111 kb on + strand, within gdhA at 1841.171 kb on - strand, within gdhA at 1841.321 kb on + strand, within gdhA at 1841.335 kb on - strand, within gdhA at 1841.335 kb on - strand, within gdhA at 1841.369 kb on - strand, within gdhA at 1841.369 kb on - strand, within gdhA at 1841.374 kb on - strand, within gdhA at 1841.458 kb on + strand, within gdhA at 1841.468 kb on + strand, within gdhA at 1841.468 kb on + strand, within gdhA at 1841.613 kb on - strand at 1841.618 kb on - strand at 1841.635 kb on - strand at 1841.653 kb on - strand at 1841.750 kb on - strand at 1841.750 kb on - strand at 1841.854 kb on + strand at 1841.854 kb on + strand at 1841.875 kb on + strand at 1841.892 kb on + strand at 1841.892 kb on + strand at 1841.900 kb on - strand at 1841.916 kb on + strand at 1841.922 kb on - strand at 1842.093 kb on - strand, within b1762 at 1842.109 kb on + strand, within b1762 at 1842.109 kb on - strand, within b1762 at 1842.174 kb on + strand, within b1762 at 1842.219 kb on + strand, within b1762 at 1842.285 kb on - strand, within b1762 at 1842.285 kb on - strand, within b1762 at 1842.285 kb on - strand, within b1762 at 1842.298 kb on - strand, within b1762 at 1842.331 kb on + strand, within b1762 at 1842.362 kb on - strand, within b1762 at 1842.362 kb on - strand, within b1762 at 1842.376 kb on - strand, within b1762 at 1842.378 kb on + strand, within b1762 at 1842.378 kb on + strand, within b1762 at 1842.380 kb on + strand, within b1762 at 1842.386 kb on - strand, within b1762 at 1842.390 kb on + strand, within b1762 at 1842.499 kb on + strand, within b1762 at 1842.541 kb on + strand, within b1762 at 1842.550 kb on + strand, within b1762 at 1842.574 kb on + strand, within b1762
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 1,839,552 - +0.0 1,839,552 - +1.0 1,839,760 - nudG b1759 0.60 -0.8 1,839,893 - +0.4 1,840,011 + ynjH b1760 0.45 +0.1 1,840,026 - ynjH b1760 0.51 -0.2 1,840,029 - ynjH b1760 0.52 -0.1 1,840,031 - ynjH b1760 0.53 -0.1 1,840,258 - -0.1 1,840,325 - -0.3 1,840,430 - +0.0 1,840,876 - gdhA b1761 0.36 +0.0 1,840,886 + gdhA b1761 0.37 -0.7 1,840,886 + gdhA b1761 0.37 +0.6 1,840,971 + gdhA b1761 0.43 +0.3 1,840,971 + gdhA b1761 0.43 -1.3 1,841,012 - gdhA b1761 0.46 +0.2 1,841,111 + gdhA b1761 0.53 -0.1 1,841,171 - gdhA b1761 0.58 +0.7 1,841,321 + gdhA b1761 0.69 +0.1 1,841,335 - gdhA b1761 0.70 -1.1 1,841,335 - gdhA b1761 0.70 +1.0 1,841,369 - gdhA b1761 0.72 +0.3 1,841,369 - gdhA b1761 0.72 -0.5 1,841,374 - gdhA b1761 0.73 +0.2 1,841,458 + gdhA b1761 0.79 +0.2 1,841,468 + gdhA b1761 0.80 +0.3 1,841,468 + gdhA b1761 0.80 +0.2 1,841,613 - +0.4 1,841,618 - +0.1 1,841,635 - -0.3 1,841,653 - -0.3 1,841,750 - -1.9 1,841,750 - -2.6 1,841,854 + -0.4 1,841,854 + -1.1 1,841,875 + -0.6 1,841,892 + -0.3 1,841,892 + +0.5 1,841,900 - -0.3 1,841,916 + +0.1 1,841,922 - -1.8 1,842,093 - b1762 b1762 0.23 +1.1 1,842,109 + b1762 b1762 0.24 +0.5 1,842,109 - b1762 b1762 0.24 -1.0 1,842,174 + b1762 b1762 0.31 +0.1 1,842,219 + b1762 b1762 0.35 +0.9 1,842,285 - b1762 b1762 0.41 +2.1 1,842,285 - b1762 b1762 0.41 +0.1 1,842,285 - b1762 b1762 0.41 +0.6 1,842,298 - b1762 b1762 0.43 +2.1 1,842,331 + b1762 b1762 0.46 +2.1 1,842,362 - b1762 b1762 0.49 -1.7 1,842,362 - b1762 b1762 0.49 -1.2 1,842,376 - b1762 b1762 0.50 +2.1 1,842,378 + b1762 b1762 0.50 +0.5 1,842,378 + b1762 b1762 0.50 +3.1 1,842,380 + b1762 b1762 0.50 +0.6 1,842,386 - b1762 b1762 0.51 +1.1 1,842,390 + b1762 b1762 0.51 +0.1 1,842,499 + b1762 b1762 0.62 +1.1 1,842,541 + b1762 b1762 0.66 -0.7 1,842,550 + b1762 b1762 0.67 -0.4 1,842,574 + b1762 b1762 0.69 +2.1
Or see this region's nucleotide sequence