Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt artQ and artI are separated by 6 nucleotides artI and artP are separated by 17 nucleotides artP and ybjP are separated by 217 nucleotides ybjP and ybjQ are separated by 125 nucleotides
b0862: artQ - arginine transporter subunit (NCBI), at 900,757 to 901,473
artQ
b0863: artI - arginine transporter subunit (NCBI), at 901,480 to 902,211
artI
b0864: artP - arginine transporter subunit (NCBI), at 902,229 to 902,957
artP
b0865: ybjP - predicted lipoprotein (NCBI), at 903,175 to 903,690
ybjP
b0866: ybjQ - hypothetical protein (NCBI), at 903,816 to 904,139
ybjQ
Position (kb) 902
903 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 901.555 kb on - strand, within artI at 901.590 kb on - strand, within artI at 901.590 kb on - strand, within artI at 901.669 kb on - strand, within artI at 901.669 kb on - strand, within artI at 901.850 kb on + strand, within artI at 901.850 kb on + strand, within artI at 901.858 kb on + strand, within artI at 901.858 kb on - strand, within artI at 901.907 kb on + strand, within artI at 902.066 kb on - strand, within artI at 902.123 kb on - strand, within artI at 902.123 kb on - strand, within artI at 902.289 kb on + strand at 902.302 kb on - strand, within artP at 902.500 kb on - strand, within artP at 902.632 kb on + strand, within artP at 902.632 kb on + strand, within artP at 902.674 kb on + strand, within artP at 902.940 kb on + strand at 902.940 kb on + strand at 902.972 kb on - strand at 903.109 kb on + strand at 903.109 kb on + strand at 903.275 kb on + strand, within ybjP at 903.299 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.641 kb on + strand at 903.651 kb on + strand at 903.695 kb on - strand at 903.842 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 901,555 - artI b0863 0.10 -0.3 901,590 - artI b0863 0.15 +0.4 901,590 - artI b0863 0.15 +0.2 901,669 - artI b0863 0.26 -0.1 901,669 - artI b0863 0.26 +0.1 901,850 + artI b0863 0.51 +0.6 901,850 + artI b0863 0.51 +0.8 901,858 + artI b0863 0.52 +0.0 901,858 - artI b0863 0.52 +0.3 901,907 + artI b0863 0.58 -0.2 902,066 - artI b0863 0.80 +0.1 902,123 - artI b0863 0.88 +0.3 902,123 - artI b0863 0.88 -0.2 902,289 + -0.1 902,302 - artP b0864 0.10 -0.3 902,500 - artP b0864 0.37 -0.3 902,632 + artP b0864 0.55 -0.6 902,632 + artP b0864 0.55 +0.1 902,674 + artP b0864 0.61 +0.3 902,940 + +0.3 902,940 + -0.4 902,972 - +0.2 903,109 + -0.0 903,109 + +0.1 903,275 + ybjP b0865 0.19 -0.9 903,299 + ybjP b0865 0.24 -0.2 903,473 + ybjP b0865 0.58 +0.2 903,473 + ybjP b0865 0.58 -2.9 903,597 - ybjP b0865 0.82 -0.8 903,597 - ybjP b0865 0.82 -1.0 903,641 + +0.0 903,651 + -1.7 903,695 - +0.5 903,842 + -0.9
Or see this region's nucleotide sequence