Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt artJ and artM are separated by 290 nucleotides artM and artQ overlap by 1 nucleotides artQ and artI are separated by 6 nucleotides
b0860: artJ - arginine transporter subunit (NCBI), at 899,067 to 899,798
artJ
b0861: artM - arginine transporter subunit (NCBI), at 900,089 to 900,757
artM
b0862: artQ - arginine transporter subunit (NCBI), at 900,757 to 901,473
artQ
b0863: artI - arginine transporter subunit (NCBI), at 901,480 to 902,211
artI
Position (kb) 900
901 Strain fitness (log2 ratio) -1
0
1 at 899.181 kb on + strand, within artJ at 899.189 kb on - strand, within artJ at 899.189 kb on - strand, within artJ at 899.335 kb on + strand, within artJ at 899.487 kb on - strand, within artJ at 899.497 kb on + strand, within artJ at 899.497 kb on + strand, within artJ at 899.515 kb on - strand, within artJ at 899.520 kb on - strand, within artJ at 899.522 kb on + strand, within artJ at 899.522 kb on + strand, within artJ at 899.684 kb on + strand, within artJ at 899.846 kb on - strand at 900.015 kb on + strand at 900.140 kb on - strand at 900.140 kb on - strand at 900.193 kb on + strand, within artM at 900.258 kb on - strand, within artM at 900.266 kb on - strand, within artM at 900.270 kb on - strand, within artM at 900.330 kb on - strand, within artM at 900.330 kb on - strand, within artM at 900.445 kb on + strand, within artM at 900.596 kb on - strand, within artM at 900.596 kb on - strand, within artM at 900.611 kb on - strand, within artM at 900.611 kb on - strand, within artM at 900.706 kb on + strand at 900.873 kb on + strand, within artQ at 901.049 kb on + strand, within artQ at 901.059 kb on + strand, within artQ at 901.088 kb on + strand, within artQ at 901.191 kb on + strand, within artQ at 901.191 kb on + strand, within artQ at 901.555 kb on - strand, within artI at 901.590 kb on - strand, within artI at 901.590 kb on - strand, within artI at 901.669 kb on - strand, within artI at 901.669 kb on - strand, within artI
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 899,181 + artJ b0860 0.16 +0.2 899,189 - artJ b0860 0.17 +0.0 899,189 - artJ b0860 0.17 -0.0 899,335 + artJ b0860 0.37 -0.4 899,487 - artJ b0860 0.57 +0.2 899,497 + artJ b0860 0.59 +0.2 899,497 + artJ b0860 0.59 -0.6 899,515 - artJ b0860 0.61 -0.5 899,520 - artJ b0860 0.62 -0.6 899,522 + artJ b0860 0.62 -0.3 899,522 + artJ b0860 0.62 -0.9 899,684 + artJ b0860 0.84 +0.0 899,846 - +0.4 900,015 + -0.8 900,140 - +0.6 900,140 - +0.2 900,193 + artM b0861 0.16 -1.4 900,258 - artM b0861 0.25 +0.0 900,266 - artM b0861 0.26 -0.3 900,270 - artM b0861 0.27 -0.1 900,330 - artM b0861 0.36 +0.1 900,330 - artM b0861 0.36 -0.0 900,445 + artM b0861 0.53 -0.7 900,596 - artM b0861 0.76 -0.9 900,596 - artM b0861 0.76 -1.4 900,611 - artM b0861 0.78 -0.6 900,611 - artM b0861 0.78 -0.4 900,706 + -0.5 900,873 + artQ b0862 0.16 -0.7 901,049 + artQ b0862 0.41 -0.7 901,059 + artQ b0862 0.42 +0.1 901,088 + artQ b0862 0.46 -0.4 901,191 + artQ b0862 0.61 +0.2 901,191 + artQ b0862 0.61 +0.4 901,555 - artI b0863 0.10 -0.3 901,590 - artI b0863 0.15 +0.4 901,590 - artI b0863 0.15 +0.2 901,669 - artI b0863 0.26 -0.1 901,669 - artI b0863 0.26 +0.1
Or see this region's nucleotide sequence