Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potE and speF overlap by 4 nucleotides speF and ybfK are separated by 122 nucleotides ybfK and kdpE are separated by 215 nucleotides
b0692: potE - putrescine/proton symporter: putrescine/ornithine antiporter (NCBI), at 716,169 to 717,488
potE
b0693: speF - ornithine decarboxylase isozyme, inducible (NCBI), at 717,485 to 719,683
speF
b4590: ybfK - hypothetical protein (RefSeq), at 719,806 to 720,063
ybfK
b0694: kdpE - DNA-binding response regulator in two-component regulatory system with KdpD (NCBI), at 720,279 to 720,956
kdpE
Position (kb) 717
718
719
720 Strain fitness (log2 ratio) -2
-1
0
1
2 at 716.522 kb on - strand, within potE at 716.663 kb on - strand, within potE at 716.702 kb on + strand, within potE at 716.710 kb on - strand, within potE at 716.763 kb on + strand, within potE at 716.763 kb on + strand, within potE at 716.788 kb on + strand, within potE at 716.788 kb on + strand, within potE at 716.868 kb on + strand, within potE at 716.982 kb on - strand, within potE at 716.997 kb on - strand, within potE at 717.074 kb on - strand, within potE at 717.096 kb on - strand, within potE at 717.157 kb on - strand, within potE at 717.157 kb on - strand, within potE at 717.225 kb on - strand, within potE at 717.225 kb on - strand, within potE at 717.442 kb on + strand at 717.442 kb on + strand at 717.442 kb on + strand at 717.444 kb on + strand at 717.498 kb on + strand at 717.498 kb on + strand at 717.538 kb on - strand at 717.545 kb on - strand at 717.585 kb on - strand at 717.636 kb on + strand at 717.660 kb on - strand at 717.660 kb on - strand at 717.679 kb on + strand at 717.684 kb on + strand at 717.736 kb on + strand, within speF at 717.736 kb on + strand, within speF at 717.760 kb on + strand, within speF at 717.815 kb on - strand at 717.815 kb on - strand, within speF at 717.872 kb on - strand, within speF at 717.961 kb on + strand, within speF at 718.010 kb on - strand, within speF at 718.010 kb on - strand, within speF at 718.313 kb on - strand, within speF at 718.339 kb on + strand, within speF at 718.408 kb on - strand, within speF at 718.426 kb on + strand, within speF at 718.443 kb on + strand, within speF at 718.473 kb on + strand, within speF at 718.473 kb on + strand, within speF at 718.506 kb on - strand, within speF at 718.553 kb on - strand, within speF at 718.639 kb on + strand, within speF at 718.670 kb on + strand, within speF at 718.716 kb on + strand, within speF at 718.729 kb on - strand, within speF at 718.729 kb on - strand, within speF at 718.733 kb on + strand, within speF at 718.749 kb on - strand, within speF at 718.829 kb on - strand, within speF at 718.852 kb on - strand, within speF at 718.982 kb on - strand, within speF at 718.982 kb on - strand, within speF at 719.054 kb on - strand, within speF at 719.054 kb on - strand, within speF at 719.059 kb on - strand, within speF at 719.059 kb on - strand, within speF at 719.141 kb on + strand, within speF at 719.141 kb on + strand, within speF at 719.154 kb on - strand, within speF at 719.219 kb on + strand, within speF at 719.230 kb on + strand, within speF at 719.261 kb on - strand, within speF at 719.288 kb on + strand, within speF at 719.293 kb on - strand, within speF at 719.293 kb on - strand, within speF at 719.308 kb on - strand, within speF at 719.313 kb on + strand, within speF at 719.313 kb on + strand, within speF at 719.321 kb on - strand, within speF at 719.321 kb on - strand, within speF at 719.321 kb on - strand, within speF at 719.331 kb on + strand, within speF at 719.333 kb on + strand, within speF at 719.341 kb on + strand, within speF at 719.341 kb on - strand, within speF at 719.356 kb on - strand, within speF at 719.370 kb on + strand, within speF at 719.404 kb on - strand, within speF at 719.408 kb on + strand, within speF at 719.432 kb on + strand, within speF at 719.459 kb on + strand, within speF at 719.459 kb on + strand, within speF at 719.470 kb on - strand at 719.470 kb on - strand at 719.471 kb on + strand at 719.480 kb on + strand at 719.511 kb on - strand at 719.554 kb on + strand at 719.592 kb on + strand at 719.632 kb on + strand at 719.632 kb on + strand at 719.636 kb on + strand at 719.636 kb on + strand at 719.815 kb on + strand at 719.829 kb on - strand at 719.829 kb on - strand at 719.911 kb on - strand, within ybfK at 719.913 kb on + strand, within ybfK at 719.915 kb on + strand, within ybfK at 719.920 kb on - strand, within ybfK at 719.921 kb on - strand, within ybfK at 719.923 kb on - strand, within ybfK at 719.926 kb on + strand, within ybfK at 720.247 kb on - strand at 720.247 kb on - strand at 720.298 kb on - strand at 720.305 kb on - strand at 720.321 kb on - strand at 720.323 kb on + strand at 720.330 kb on - strand at 720.330 kb on - strand at 720.331 kb on - strand at 720.332 kb on - strand at 720.332 kb on - strand at 720.336 kb on - strand at 720.358 kb on - strand, within kdpE at 720.391 kb on - strand, within kdpE at 720.403 kb on - strand, within kdpE at 720.420 kb on - strand, within kdpE at 720.441 kb on + strand, within kdpE at 720.441 kb on + strand, within kdpE at 720.449 kb on - strand, within kdpE at 720.481 kb on - strand, within kdpE at 720.512 kb on + strand, within kdpE at 720.512 kb on + strand, within kdpE at 720.627 kb on + strand, within kdpE at 720.678 kb on + strand, within kdpE at 720.678 kb on + strand, within kdpE
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 716,522 - potE b0692 0.27 -0.1 716,663 - potE b0692 0.37 -0.1 716,702 + potE b0692 0.40 -0.8 716,710 - potE b0692 0.41 -0.1 716,763 + potE b0692 0.45 -0.3 716,763 + potE b0692 0.45 -0.1 716,788 + potE b0692 0.47 +1.6 716,788 + potE b0692 0.47 -0.8 716,868 + potE b0692 0.53 +0.0 716,982 - potE b0692 0.62 -0.7 716,997 - potE b0692 0.63 -0.0 717,074 - potE b0692 0.69 -0.0 717,096 - potE b0692 0.70 -0.0 717,157 - potE b0692 0.75 +0.6 717,157 - potE b0692 0.75 +2.3 717,225 - potE b0692 0.80 -0.0 717,225 - potE b0692 0.80 -0.4 717,442 + +0.0 717,442 + -1.7 717,442 + -0.3 717,444 + -0.4 717,498 + +0.5 717,498 + -0.0 717,538 - +0.1 717,545 - -0.3 717,585 - -0.5 717,636 + +1.0 717,660 - -0.6 717,660 - -0.5 717,679 + +0.3 717,684 + -0.2 717,736 + speF b0693 0.11 +0.1 717,736 + speF b0693 0.11 -0.3 717,760 + speF b0693 0.13 +0.3 717,815 - -2.0 717,815 - speF b0693 0.15 -0.5 717,872 - speF b0693 0.18 -0.4 717,961 + speF b0693 0.22 -0.3 718,010 - speF b0693 0.24 -0.3 718,010 - speF b0693 0.24 -0.2 718,313 - speF b0693 0.38 -0.4 718,339 + speF b0693 0.39 -0.4 718,408 - speF b0693 0.42 +0.2 718,426 + speF b0693 0.43 -0.2 718,443 + speF b0693 0.44 +0.1 718,473 + speF b0693 0.45 +0.5 718,473 + speF b0693 0.45 -0.2 718,506 - speF b0693 0.46 +0.2 718,553 - speF b0693 0.49 +0.1 718,639 + speF b0693 0.52 +0.1 718,670 + speF b0693 0.54 -0.7 718,716 + speF b0693 0.56 +0.1 718,729 - speF b0693 0.57 -0.3 718,729 - speF b0693 0.57 -0.6 718,733 + speF b0693 0.57 +0.1 718,749 - speF b0693 0.57 -0.9 718,829 - speF b0693 0.61 +0.0 718,852 - speF b0693 0.62 -0.5 718,982 - speF b0693 0.68 -0.2 718,982 - speF b0693 0.68 +0.2 719,054 - speF b0693 0.71 +0.7 719,054 - speF b0693 0.71 +0.2 719,059 - speF b0693 0.72 -0.8 719,059 - speF b0693 0.72 -0.4 719,141 + speF b0693 0.75 -0.2 719,141 + speF b0693 0.75 -0.3 719,154 - speF b0693 0.76 -0.7 719,219 + speF b0693 0.79 +0.7 719,230 + speF b0693 0.79 +0.0 719,261 - speF b0693 0.81 -0.2 719,288 + speF b0693 0.82 -0.5 719,293 - speF b0693 0.82 -0.5 719,293 - speF b0693 0.82 +0.2 719,308 - speF b0693 0.83 -0.1 719,313 + speF b0693 0.83 -0.4 719,313 + speF b0693 0.83 -0.3 719,321 - speF b0693 0.83 -0.2 719,321 - speF b0693 0.83 -0.2 719,321 - speF b0693 0.83 +0.1 719,331 + speF b0693 0.84 -2.3 719,333 + speF b0693 0.84 -0.4 719,341 + speF b0693 0.84 +0.4 719,341 - speF b0693 0.84 +0.4 719,356 - speF b0693 0.85 +2.5 719,370 + speF b0693 0.86 -0.5 719,404 - speF b0693 0.87 +2.3 719,408 + speF b0693 0.87 +0.4 719,432 + speF b0693 0.89 +0.4 719,459 + speF b0693 0.90 +0.6 719,459 + speF b0693 0.90 +0.1 719,470 - -0.9 719,470 - -0.7 719,471 + +0.7 719,480 + +0.7 719,511 - +1.9 719,554 + -1.6 719,592 + -0.7 719,632 + +1.9 719,632 + +0.9 719,636 + +0.9 719,636 + -0.3 719,815 + +1.9 719,829 - -0.1 719,829 - +0.8 719,911 - ybfK b4590 0.41 +1.9 719,913 + ybfK b4590 0.41 +1.9 719,915 + ybfK b4590 0.42 +1.9 719,920 - ybfK b4590 0.44 +0.1 719,921 - ybfK b4590 0.45 +1.9 719,923 - ybfK b4590 0.45 +1.9 719,926 + ybfK b4590 0.47 +1.9 720,247 - +1.1 720,247 - +0.1 720,298 - -0.4 720,305 - -0.1 720,321 - +0.4 720,323 + +1.9 720,330 - +0.4 720,330 - -0.4 720,331 - -0.4 720,332 - -0.1 720,332 - +0.8 720,336 - +0.6 720,358 - kdpE b0694 0.12 -0.2 720,391 - kdpE b0694 0.17 +1.1 720,403 - kdpE b0694 0.18 -0.5 720,420 - kdpE b0694 0.21 -0.1 720,441 + kdpE b0694 0.24 -0.2 720,441 + kdpE b0694 0.24 -0.9 720,449 - kdpE b0694 0.25 -0.1 720,481 - kdpE b0694 0.30 +0.2 720,512 + kdpE b0694 0.34 -0.1 720,512 + kdpE b0694 0.34 -0.7 720,627 + kdpE b0694 0.51 +0.1 720,678 + kdpE b0694 0.59 +0.0 720,678 + kdpE b0694 0.59 -0.6
Or see this region's nucleotide sequence