Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ompT and pauD are separated by 423 nucleotides pauD and envY are separated by 45 nucleotides envY and ybcH are separated by 182 nucleotides
b0565: ompT - DLP12 prophage; outer membrane protease VII (outer membrane protein 3b) (NCBI), at 583,903 to 584,856
ompT
b4635: pauD - tRNA-OTHER (RefSeq), at 585,280 to 585,324
pauD
b0566: envY - DNA-binding transcriptional activator of porin biosynthesis (NCBI), at 585,370 to 586,131
envY
b0567: ybcH - hypothetical protein (NCBI), at 586,314 to 587,204
ybcH
Position (kb) 585
586
587 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 584.370 kb on - strand, within ompT at 584.370 kb on - strand, within ompT at 584.371 kb on - strand, within ompT at 584.377 kb on + strand, within ompT at 584.391 kb on + strand, within ompT at 584.398 kb on - strand, within ompT at 584.399 kb on - strand, within ompT at 584.407 kb on + strand, within ompT at 584.415 kb on + strand, within ompT at 584.423 kb on - strand, within ompT at 584.423 kb on - strand, within ompT at 584.444 kb on - strand, within ompT at 584.492 kb on - strand, within ompT at 584.492 kb on - strand, within ompT at 584.494 kb on - strand, within ompT at 584.494 kb on - strand, within ompT at 584.500 kb on - strand, within ompT at 584.500 kb on - strand, within ompT at 584.516 kb on + strand, within ompT at 584.524 kb on - strand, within ompT at 584.524 kb on - strand, within ompT at 584.531 kb on + strand, within ompT at 584.537 kb on + strand, within ompT at 584.537 kb on + strand, within ompT at 584.537 kb on + strand, within ompT at 584.545 kb on - strand, within ompT at 584.564 kb on - strand, within ompT at 584.564 kb on - strand, within ompT at 584.565 kb on - strand, within ompT at 584.566 kb on - strand, within ompT at 584.566 kb on - strand, within ompT at 584.572 kb on + strand, within ompT at 584.572 kb on + strand, within ompT at 584.577 kb on - strand, within ompT at 584.580 kb on + strand, within ompT at 584.581 kb on + strand, within ompT at 584.582 kb on + strand, within ompT at 584.584 kb on - strand, within ompT at 584.587 kb on + strand, within ompT at 584.587 kb on + strand, within ompT at 584.589 kb on + strand, within ompT at 584.590 kb on - strand, within ompT at 584.608 kb on - strand, within ompT at 584.612 kb on - strand, within ompT at 584.612 kb on - strand, within ompT at 584.689 kb on + strand, within ompT at 584.704 kb on - strand, within ompT at 584.714 kb on - strand, within ompT at 584.714 kb on - strand, within ompT at 584.736 kb on + strand, within ompT at 584.736 kb on + strand, within ompT at 584.747 kb on + strand, within ompT at 584.767 kb on - strand at 584.786 kb on + strand at 584.786 kb on + strand at 584.796 kb on + strand at 584.814 kb on + strand at 584.814 kb on + strand at 584.822 kb on - strand at 584.822 kb on - strand at 584.822 kb on - strand at 584.822 kb on - strand at 584.832 kb on - strand at 584.844 kb on - strand at 584.861 kb on - strand at 584.930 kb on - strand at 584.931 kb on - strand at 584.972 kb on - strand at 584.979 kb on - strand at 585.077 kb on + strand at 585.093 kb on - strand at 585.111 kb on - strand at 585.128 kb on - strand at 585.128 kb on - strand at 585.179 kb on - strand at 585.181 kb on + strand at 585.216 kb on - strand at 585.232 kb on - strand at 585.244 kb on - strand at 585.244 kb on - strand at 585.335 kb on + strand at 585.346 kb on + strand at 585.346 kb on + strand at 585.353 kb on - strand at 585.368 kb on + strand at 585.368 kb on + strand at 585.373 kb on - strand at 585.527 kb on + strand, within envY at 585.527 kb on - strand, within envY at 585.527 kb on - strand, within envY at 585.533 kb on + strand, within envY at 585.533 kb on + strand, within envY at 585.541 kb on - strand, within envY at 585.542 kb on - strand, within envY at 585.566 kb on - strand, within envY at 585.566 kb on - strand, within envY at 585.600 kb on + strand, within envY at 585.600 kb on + strand, within envY at 585.600 kb on + strand, within envY at 585.600 kb on + strand, within envY at 585.608 kb on - strand, within envY at 585.608 kb on - strand, within envY at 585.614 kb on - strand, within envY at 585.649 kb on - strand, within envY at 585.649 kb on - strand, within envY at 585.649 kb on - strand, within envY at 585.685 kb on + strand, within envY at 585.685 kb on + strand, within envY at 585.703 kb on - strand, within envY at 585.715 kb on - strand, within envY at 585.833 kb on + strand, within envY at 585.857 kb on - strand, within envY at 585.963 kb on - strand, within envY at 585.963 kb on - strand, within envY at 585.972 kb on - strand, within envY at 586.074 kb on + strand at 586.123 kb on - strand at 586.150 kb on + strand at 586.305 kb on - strand at 586.312 kb on + strand at 586.312 kb on + strand at 586.528 kb on - strand, within ybcH at 586.541 kb on + strand, within ybcH at 586.541 kb on - strand, within ybcH at 586.544 kb on - strand, within ybcH at 586.544 kb on - strand, within ybcH at 586.574 kb on + strand, within ybcH at 586.627 kb on - strand, within ybcH at 586.794 kb on + strand, within ybcH at 586.794 kb on + strand, within ybcH at 586.834 kb on - strand, within ybcH at 586.834 kb on - strand, within ybcH at 587.002 kb on - strand, within ybcH at 587.062 kb on + strand, within ybcH at 587.073 kb on + strand, within ybcH at 587.073 kb on + strand, within ybcH at 587.081 kb on - strand, within ybcH at 587.114 kb on + strand, within ybcH
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 584,370 - ompT b0565 0.49 +0.1 584,370 - ompT b0565 0.49 -0.1 584,371 - ompT b0565 0.49 -0.8 584,377 + ompT b0565 0.50 -0.5 584,391 + ompT b0565 0.51 +3.8 584,398 - ompT b0565 0.52 -0.4 584,399 - ompT b0565 0.52 -0.2 584,407 + ompT b0565 0.53 +0.2 584,415 + ompT b0565 0.54 +1.8 584,423 - ompT b0565 0.55 -0.6 584,423 - ompT b0565 0.55 +0.1 584,444 - ompT b0565 0.57 +0.0 584,492 - ompT b0565 0.62 -1.2 584,492 - ompT b0565 0.62 +0.5 584,494 - ompT b0565 0.62 -0.4 584,494 - ompT b0565 0.62 -0.1 584,500 - ompT b0565 0.63 +0.2 584,500 - ompT b0565 0.63 -0.8 584,516 + ompT b0565 0.64 -0.2 584,524 - ompT b0565 0.65 +0.2 584,524 - ompT b0565 0.65 +0.2 584,531 + ompT b0565 0.66 +0.8 584,537 + ompT b0565 0.66 +1.8 584,537 + ompT b0565 0.66 -0.5 584,537 + ompT b0565 0.66 +0.0 584,545 - ompT b0565 0.67 -1.3 584,564 - ompT b0565 0.69 -0.1 584,564 - ompT b0565 0.69 +0.2 584,565 - ompT b0565 0.69 +0.8 584,566 - ompT b0565 0.69 -0.9 584,566 - ompT b0565 0.69 +0.1 584,572 + ompT b0565 0.70 +1.3 584,572 + ompT b0565 0.70 -0.6 584,577 - ompT b0565 0.71 -1.1 584,580 + ompT b0565 0.71 -0.2 584,581 + ompT b0565 0.71 +0.2 584,582 + ompT b0565 0.71 +0.8 584,584 - ompT b0565 0.71 +0.2 584,587 + ompT b0565 0.72 +0.1 584,587 + ompT b0565 0.72 -0.3 584,589 + ompT b0565 0.72 +0.2 584,590 - ompT b0565 0.72 -1.0 584,608 - ompT b0565 0.74 -0.4 584,612 - ompT b0565 0.74 -0.3 584,612 - ompT b0565 0.74 -0.2 584,689 + ompT b0565 0.82 -1.5 584,704 - ompT b0565 0.84 +0.2 584,714 - ompT b0565 0.85 +0.8 584,714 - ompT b0565 0.85 +0.6 584,736 + ompT b0565 0.87 -0.0 584,736 + ompT b0565 0.87 -0.8 584,747 + ompT b0565 0.88 -0.3 584,767 - +0.2 584,786 + +2.8 584,786 + -0.5 584,796 + +1.8 584,814 + +1.8 584,814 + +0.8 584,822 - -0.9 584,822 - +0.7 584,822 - +1.1 584,822 - +0.0 584,832 - +0.0 584,844 - +0.4 584,861 - +0.3 584,930 - -0.1 584,931 - -0.3 584,972 - -0.8 584,979 - -1.3 585,077 + +1.2 585,093 - +0.2 585,111 - -0.4 585,128 - -0.3 585,128 - +0.9 585,179 - -0.1 585,181 + +1.8 585,216 - -0.2 585,232 - -0.3 585,244 - +0.1 585,244 - +0.6 585,335 + +1.8 585,346 + +1.8 585,346 + +1.2 585,353 - +0.4 585,368 + -0.8 585,368 + +1.1 585,373 - -0.1 585,527 + envY b0566 0.21 -0.2 585,527 - envY b0566 0.21 +0.0 585,527 - envY b0566 0.21 +0.4 585,533 + envY b0566 0.21 +0.5 585,533 + envY b0566 0.21 +1.2 585,541 - envY b0566 0.22 -0.0 585,542 - envY b0566 0.23 -0.3 585,566 - envY b0566 0.26 +0.2 585,566 - envY b0566 0.26 -0.6 585,600 + envY b0566 0.30 +2.6 585,600 + envY b0566 0.30 +0.2 585,600 + envY b0566 0.30 -0.5 585,600 + envY b0566 0.30 -0.4 585,608 - envY b0566 0.31 -0.3 585,608 - envY b0566 0.31 -0.2 585,614 - envY b0566 0.32 -0.4 585,649 - envY b0566 0.37 -0.5 585,649 - envY b0566 0.37 +0.2 585,649 - envY b0566 0.37 -0.1 585,685 + envY b0566 0.41 +0.6 585,685 + envY b0566 0.41 +1.8 585,703 - envY b0566 0.44 +0.2 585,715 - envY b0566 0.45 -0.1 585,833 + envY b0566 0.61 +0.7 585,857 - envY b0566 0.64 +0.4 585,963 - envY b0566 0.78 -0.7 585,963 - envY b0566 0.78 -0.4 585,972 - envY b0566 0.79 -0.1 586,074 + -1.6 586,123 - -0.2 586,150 + +0.7 586,305 - -0.2 586,312 + +0.3 586,312 + -0.6 586,528 - ybcH b0567 0.24 -0.4 586,541 + ybcH b0567 0.25 -0.0 586,541 - ybcH b0567 0.25 -0.7 586,544 - ybcH b0567 0.26 -0.2 586,544 - ybcH b0567 0.26 -0.4 586,574 + ybcH b0567 0.29 -0.6 586,627 - ybcH b0567 0.35 +0.3 586,794 + ybcH b0567 0.54 +0.6 586,794 + ybcH b0567 0.54 -0.8 586,834 - ybcH b0567 0.58 -0.0 586,834 - ybcH b0567 0.58 -0.0 587,002 - ybcH b0567 0.77 +0.1 587,062 + ybcH b0567 0.84 -0.2 587,073 + ybcH b0567 0.85 +0.9 587,073 + ybcH b0567 0.85 -0.5 587,081 - ybcH b0567 0.86 -0.2 587,114 + ybcH b0567 0.90 -0.3
Or see this region's nucleotide sequence