Experiment: Outgrowth from conjugation with pRH23 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylbE and ylbF are separated by 10 nucleotides ylbF and ybcF overlap by 4 nucleotides ybcF and purK are separated by 194 nucleotides
b4572: ylbE - no description, at 547,581 to 548,839
ylbE
b0520: ylbF - hypothetical protein (NCBI), at 548,850 to 549,665
ylbF
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
Position (kb) 549
550
551 Strain fitness (log2 ratio) -2
-1
0
1 at 548.700 kb on + strand, within ylbE at 548.708 kb on - strand, within ylbE at 548.713 kb on - strand, within ylbE at 548.718 kb on - strand at 548.755 kb on + strand at 548.833 kb on + strand at 549.010 kb on + strand, within ylbF at 549.010 kb on + strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.219 kb on + strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.392 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.458 kb on + strand, within ylbF at 549.488 kb on + strand, within ylbF at 549.491 kb on - strand, within ylbF at 549.534 kb on + strand, within ylbF at 549.625 kb on + strand at 549.695 kb on + strand at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.954 kb on - strand, within ybcF at 550.141 kb on - strand, within ybcF at 550.363 kb on + strand, within ybcF at 550.471 kb on - strand at 550.539 kb on + strand at 550.550 kb on - strand at 550.563 kb on + strand at 550.563 kb on - strand at 550.607 kb on + strand at 550.677 kb on + strand at 550.697 kb on - strand at 550.746 kb on + strand at 550.755 kb on - strand at 550.765 kb on - strand at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH23 in LB+Gent50 remove 548,700 + ylbE b4572 0.89 -0.5 548,708 - ylbE b4572 0.90 -0.4 548,713 - ylbE b4572 0.90 -0.3 548,718 - -0.1 548,755 + +0.0 548,833 + +0.0 549,010 + ylbF b0520 0.20 -0.1 549,010 + ylbF b0520 0.20 -0.1 549,046 - ylbF b0520 0.24 -0.1 549,046 - ylbF b0520 0.24 -0.1 549,219 + ylbF b0520 0.45 -0.1 549,354 - ylbF b0520 0.62 -0.5 549,354 - ylbF b0520 0.62 +0.8 549,392 + ylbF b0520 0.66 -0.1 549,450 + ylbF b0520 0.74 +0.6 549,450 + ylbF b0520 0.74 -0.6 549,458 + ylbF b0520 0.75 -0.8 549,488 + ylbF b0520 0.78 -0.4 549,491 - ylbF b0520 0.79 -0.2 549,534 + ylbF b0520 0.84 -1.5 549,625 + +0.5 549,695 + -0.0 549,755 + ybcF b0521 0.10 +0.1 549,755 + ybcF b0521 0.10 +0.1 549,764 + ybcF b0521 0.11 +0.6 549,811 - ybcF b0521 0.17 -0.7 549,933 + ybcF b0521 0.30 -0.1 549,933 + ybcF b0521 0.30 +0.9 549,954 - ybcF b0521 0.33 -0.4 550,141 - ybcF b0521 0.54 -0.3 550,363 + ybcF b0521 0.78 +1.0 550,471 - +0.7 550,539 + -0.4 550,550 - -0.2 550,563 + -0.4 550,563 - +0.4 550,607 + +0.4 550,677 + -0.1 550,697 - +0.7 550,746 + -0.3 550,755 - +0.0 550,765 - -0.1 550,959 + purK b0522 0.20 -0.3 550,977 - purK b0522 0.21 -0.3 551,057 + purK b0522 0.29 -0.1 551,062 + purK b0522 0.29 -0.8 551,062 + purK b0522 0.29 -0.3 551,091 - purK b0522 0.32 -0.6 551,095 + purK b0522 0.32 +0.6 551,095 + purK b0522 0.32 -0.4 551,112 + purK b0522 0.34 -0.0 551,112 + purK b0522 0.34 -2.5 551,144 + purK b0522 0.37 -0.4 551,162 - purK b0522 0.39 +0.5 551,162 - purK b0522 0.39 +0.5 551,217 + purK b0522 0.44 -1.0 551,227 - purK b0522 0.45 -0.8 551,236 + purK b0522 0.46 -0.5 551,236 + purK b0522 0.46 -0.6 551,255 + purK b0522 0.47 -0.9 551,271 + purK b0522 0.49 -0.1 551,356 + purK b0522 0.57 -0.2 551,375 + purK b0522 0.59 -0.7 551,391 - purK b0522 0.60 +0.5 551,458 - purK b0522 0.66 -0.4 551,532 + purK b0522 0.73 -0.5 551,532 + purK b0522 0.73 -0.1 551,543 + purK b0522 0.74 -1.3
Or see this region's nucleotide sequence