Strain Fitness in Escherichia coli BW25113 around b4186

Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjfL and yjfM are separated by 9 nucleotidesyjfM and yjfC are separated by 2 nucleotidesyjfC and aidB are separated by 83 nucleotides b4184: yjfL - conserved inner membrane protein (NCBI), at 4,410,002 to 4,410,400 yjfL b4185: yjfM - hypothetical protein (NCBI), at 4,410,410 to 4,411,048 yjfM b4186: yjfC - predicted synthetase/amidase (NCBI), at 4,411,051 to 4,412,214 yjfC b4187: aidB - putative acyl coenzyme A dehydrogenase (VIMSS), at 4,412,298 to 4,413,923 aidB Position (kb) 4411 4412 4413Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 4410.164 kb on - strand, within yjfLat 4410.164 kb on - strand, within yjfLat 4410.167 kb on - strand, within yjfLat 4410.167 kb on - strand, within yjfLat 4410.177 kb on - strand, within yjfLat 4410.177 kb on - strand, within yjfLat 4410.182 kb on + strand, within yjfLat 4410.182 kb on + strand, within yjfLat 4410.184 kb on - strand, within yjfLat 4410.189 kb on + strand, within yjfLat 4410.190 kb on - strand, within yjfLat 4410.190 kb on - strand, within yjfLat 4410.190 kb on - strand, within yjfLat 4410.190 kb on - strand, within yjfLat 4410.190 kb on - strand, within yjfLat 4410.195 kb on - strand, within yjfLat 4410.206 kb on - strand, within yjfLat 4410.207 kb on - strand, within yjfLat 4410.215 kb on + strand, within yjfLat 4410.221 kb on - strand, within yjfLat 4410.228 kb on - strand, within yjfLat 4410.228 kb on - strand, within yjfLat 4410.248 kb on + strand, within yjfLat 4410.256 kb on - strand, within yjfLat 4410.282 kb on + strand, within yjfLat 4410.294 kb on - strand, within yjfLat 4410.379 kb on - strandat 4410.385 kb on + strandat 4410.392 kb on + strandat 4410.393 kb on - strandat 4410.393 kb on - strandat 4410.395 kb on - strandat 4410.400 kb on - strandat 4410.400 kb on - strandat 4410.404 kb on - strandat 4410.405 kb on - strandat 4410.405 kb on - strandat 4410.405 kb on - strandat 4410.407 kb on + strandat 4410.407 kb on + strandat 4410.407 kb on + strandat 4410.447 kb on - strandat 4410.471 kb on + strandat 4410.477 kb on - strand, within yjfMat 4410.477 kb on - strand, within yjfMat 4410.479 kb on - strand, within yjfMat 4410.489 kb on - strand, within yjfMat 4410.497 kb on - strand, within yjfMat 4410.515 kb on - strand, within yjfMat 4410.515 kb on - strand, within yjfMat 4410.539 kb on + strand, within yjfMat 4410.547 kb on - strand, within yjfMat 4410.547 kb on - strand, within yjfMat 4410.549 kb on - strand, within yjfMat 4410.575 kb on + strand, within yjfMat 4410.579 kb on - strand, within yjfMat 4410.590 kb on - strand, within yjfMat 4410.591 kb on - strand, within yjfMat 4410.632 kb on - strand, within yjfMat 4410.637 kb on - strand, within yjfMat 4410.637 kb on - strand, within yjfMat 4410.637 kb on - strand, within yjfMat 4410.637 kb on - strand, within yjfMat 4410.642 kb on - strand, within yjfMat 4410.647 kb on - strand, within yjfMat 4410.647 kb on - strand, within yjfMat 4410.647 kb on - strand, within yjfMat 4410.647 kb on - strand, within yjfMat 4410.659 kb on - strand, within yjfMat 4410.685 kb on - strand, within yjfMat 4410.685 kb on - strand, within yjfMat 4410.691 kb on - strand, within yjfMat 4410.731 kb on + strand, within yjfMat 4410.731 kb on + strand, within yjfMat 4410.745 kb on + strand, within yjfMat 4410.745 kb on + strand, within yjfMat 4410.753 kb on - strand, within yjfMat 4410.784 kb on - strand, within yjfMat 4410.792 kb on - strand, within yjfMat 4410.854 kb on + strand, within yjfMat 4410.862 kb on - strand, within yjfMat 4410.862 kb on - strand, within yjfMat 4410.876 kb on + strand, within yjfMat 4410.891 kb on - strand, within yjfMat 4410.893 kb on + strand, within yjfMat 4410.906 kb on + strand, within yjfMat 4410.906 kb on + strand, within yjfMat 4410.955 kb on + strand, within yjfMat 4411.147 kb on + strandat 4411.260 kb on - strand, within yjfCat 4411.298 kb on + strand, within yjfCat 4411.298 kb on + strand, within yjfCat 4411.357 kb on + strand, within yjfCat 4411.376 kb on - strand, within yjfCat 4411.398 kb on + strand, within yjfCat 4411.398 kb on + strand, within yjfCat 4411.579 kb on + strand, within yjfCat 4411.585 kb on - strand, within yjfCat 4411.642 kb on + strand, within yjfCat 4411.643 kb on - strand, within yjfCat 4411.704 kb on - strand, within yjfCat 4411.704 kb on - strand, within yjfCat 4411.730 kb on - strand, within yjfCat 4411.740 kb on + strand, within yjfCat 4411.805 kb on + strand, within yjfCat 4411.863 kb on - strand, within yjfCat 4411.863 kb on - strand, within yjfCat 4411.872 kb on + strand, within yjfCat 4411.900 kb on - strand, within yjfCat 4411.900 kb on - strand, within yjfCat 4411.904 kb on + strand, within yjfCat 4411.904 kb on + strand, within yjfCat 4411.958 kb on - strand, within yjfCat 4412.034 kb on - strand, within yjfCat 4412.064 kb on + strand, within yjfCat 4412.064 kb on + strand, within yjfCat 4412.101 kb on - strandat 4412.101 kb on - strandat 4412.146 kb on - strandat 4412.246 kb on - strandat 4412.252 kb on + strandat 4412.270 kb on - strandat 4412.282 kb on - strandat 4412.286 kb on + strandat 4412.319 kb on + strandat 4412.338 kb on - strandat 4412.338 kb on - strandat 4412.360 kb on - strandat 4412.378 kb on + strandat 4412.449 kb on - strandat 4412.449 kb on - strandat 4412.564 kb on - strand, within aidBat 4412.611 kb on + strand, within aidBat 4412.611 kb on + strand, within aidBat 4412.670 kb on + strand, within aidBat 4412.670 kb on + strand, within aidBat 4412.712 kb on - strand, within aidBat 4412.793 kb on - strand, within aidBat 4412.901 kb on - strand, within aidBat 4412.908 kb on - strand, within aidBat 4413.002 kb on + strand, within aidBat 4413.002 kb on + strand, within aidBat 4413.088 kb on + strand, within aidBat 4413.088 kb on - strand, within aidBat 4413.123 kb on + strand, within aidB

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Per-strain Table

Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
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4,410,164 - yjfL b4184 0.41 -0.7
4,410,164 - yjfL b4184 0.41 +0.6
4,410,167 - yjfL b4184 0.41 -2.5
4,410,167 - yjfL b4184 0.41 +2.2
4,410,177 - yjfL b4184 0.44 -1.4
4,410,177 - yjfL b4184 0.44 +0.8
4,410,182 + yjfL b4184 0.45 +1.6
4,410,182 + yjfL b4184 0.45 +0.0
4,410,184 - yjfL b4184 0.46 +0.5
4,410,189 + yjfL b4184 0.47 +2.6
4,410,190 - yjfL b4184 0.47 -0.4
4,410,190 - yjfL b4184 0.47 -0.4
4,410,190 - yjfL b4184 0.47 +0.4
4,410,190 - yjfL b4184 0.47 +3.2
4,410,190 - yjfL b4184 0.47 -2.2
4,410,195 - yjfL b4184 0.48 +0.4
4,410,206 - yjfL b4184 0.51 +2.8
4,410,207 - yjfL b4184 0.51 +0.6
4,410,215 + yjfL b4184 0.53 -0.5
4,410,221 - yjfL b4184 0.55 +1.6
4,410,228 - yjfL b4184 0.57 +0.6
4,410,228 - yjfL b4184 0.57 +2.2
4,410,248 + yjfL b4184 0.62 -1.4
4,410,256 - yjfL b4184 0.64 -1.7
4,410,282 + yjfL b4184 0.70 +1.9
4,410,294 - yjfL b4184 0.73 +0.0
4,410,379 - -1.0
4,410,385 + -1.1
4,410,392 + +0.6
4,410,393 - -0.5
4,410,393 - +0.0
4,410,395 - +0.2
4,410,400 - -0.8
4,410,400 - +0.3
4,410,404 - +0.3
4,410,405 - -0.1
4,410,405 - -1.7
4,410,405 - -2.0
4,410,407 + +1.6
4,410,407 + -2.0
4,410,407 + -2.1
4,410,447 - -0.4
4,410,471 + -0.6
4,410,477 - yjfM b4185 0.10 +1.2
4,410,477 - yjfM b4185 0.10 +0.1
4,410,479 - yjfM b4185 0.11 +0.7
4,410,489 - yjfM b4185 0.12 +1.6
4,410,497 - yjfM b4185 0.14 -1.1
4,410,515 - yjfM b4185 0.16 +0.1
4,410,515 - yjfM b4185 0.16 +0.4
4,410,539 + yjfM b4185 0.20 +2.3
4,410,547 - yjfM b4185 0.21 -0.5
4,410,547 - yjfM b4185 0.21 -0.6
4,410,549 - yjfM b4185 0.22 +1.6
4,410,575 + yjfM b4185 0.26 -1.1
4,410,579 - yjfM b4185 0.26 -0.1
4,410,590 - yjfM b4185 0.28 +0.2
4,410,591 - yjfM b4185 0.28 +0.1
4,410,632 - yjfM b4185 0.35 -0.5
4,410,637 - yjfM b4185 0.36 -1.1
4,410,637 - yjfM b4185 0.36 -0.5
4,410,637 - yjfM b4185 0.36 +0.0
4,410,637 - yjfM b4185 0.36 -0.9
4,410,642 - yjfM b4185 0.36 +0.7
4,410,647 - yjfM b4185 0.37 -0.1
4,410,647 - yjfM b4185 0.37 +0.2
4,410,647 - yjfM b4185 0.37 -0.6
4,410,647 - yjfM b4185 0.37 -1.4
4,410,659 - yjfM b4185 0.39 +0.6
4,410,685 - yjfM b4185 0.43 -0.0
4,410,685 - yjfM b4185 0.43 +0.3
4,410,691 - yjfM b4185 0.44 -0.2
4,410,731 + yjfM b4185 0.50 -1.3
4,410,731 + yjfM b4185 0.50 +0.0
4,410,745 + yjfM b4185 0.52 +0.5
4,410,745 + yjfM b4185 0.52 -0.4
4,410,753 - yjfM b4185 0.54 -0.6
4,410,784 - yjfM b4185 0.59 -1.2
4,410,792 - yjfM b4185 0.60 -0.0
4,410,854 + yjfM b4185 0.69 +0.4
4,410,862 - yjfM b4185 0.71 -0.2
4,410,862 - yjfM b4185 0.71 -2.2
4,410,876 + yjfM b4185 0.73 -0.2
4,410,891 - yjfM b4185 0.75 -0.6
4,410,893 + yjfM b4185 0.76 +0.9
4,410,906 + yjfM b4185 0.78 -0.4
4,410,906 + yjfM b4185 0.78 -0.1
4,410,955 + yjfM b4185 0.85 +0.3
4,411,147 + -0.3
4,411,260 - yjfC b4186 0.18 +4.8
4,411,298 + yjfC b4186 0.21 +1.2
4,411,298 + yjfC b4186 0.21 +0.0
4,411,357 + yjfC b4186 0.26 -0.2
4,411,376 - yjfC b4186 0.28 -0.3
4,411,398 + yjfC b4186 0.30 +0.5
4,411,398 + yjfC b4186 0.30 +0.3
4,411,579 + yjfC b4186 0.45 +0.6
4,411,585 - yjfC b4186 0.46 -0.7
4,411,642 + yjfC b4186 0.51 +0.4
4,411,643 - yjfC b4186 0.51 +0.1
4,411,704 - yjfC b4186 0.56 -1.2
4,411,704 - yjfC b4186 0.56 -0.2
4,411,730 - yjfC b4186 0.58 -0.3
4,411,740 + yjfC b4186 0.59 -0.2
4,411,805 + yjfC b4186 0.65 +0.5
4,411,863 - yjfC b4186 0.70 +0.4
4,411,863 - yjfC b4186 0.70 +0.5
4,411,872 + yjfC b4186 0.71 -0.2
4,411,900 - yjfC b4186 0.73 +2.0
4,411,900 - yjfC b4186 0.73 +0.5
4,411,904 + yjfC b4186 0.73 +0.4
4,411,904 + yjfC b4186 0.73 +0.6
4,411,958 - yjfC b4186 0.78 +0.2
4,412,034 - yjfC b4186 0.84 -0.8
4,412,064 + yjfC b4186 0.87 +1.0
4,412,064 + yjfC b4186 0.87 -0.2
4,412,101 - -0.3
4,412,101 - -0.5
4,412,146 - -0.1
4,412,246 - +0.2
4,412,252 + -0.1
4,412,270 - -0.8
4,412,282 - +0.4
4,412,286 + -0.1
4,412,319 + +0.3
4,412,338 - +0.2
4,412,338 - +0.1
4,412,360 - +0.5
4,412,378 + +0.3
4,412,449 - +0.5
4,412,449 - +0.1
4,412,564 - aidB b4187 0.16 +0.4
4,412,611 + aidB b4187 0.19 -0.3
4,412,611 + aidB b4187 0.19 +0.8
4,412,670 + aidB b4187 0.23 -0.2
4,412,670 + aidB b4187 0.23 +0.0
4,412,712 - aidB b4187 0.25 -0.8
4,412,793 - aidB b4187 0.30 +0.0
4,412,901 - aidB b4187 0.37 -0.2
4,412,908 - aidB b4187 0.38 -0.2
4,413,002 + aidB b4187 0.43 +0.2
4,413,002 + aidB b4187 0.43 +0.5
4,413,088 + aidB b4187 0.49 -0.4
4,413,088 - aidB b4187 0.49 -0.4
4,413,123 + aidB b4187 0.51 +0.7

Or see this region's nucleotide sequence