Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lacY and lacZ are separated by 51 nucleotides lacZ and lacI are separated by 122 nucleotides
b0343: lacY - galactoside permease (NCBI), at 361,150 to 362,403
lacY
b0344: lacZ - beta-D-galactosidase (NCBI), at 362,455 to 365,529
lacZ
b0345: lacI - transcriptional repressor of the lac operon (VIMSS), at 365,652 to 366,734
lacI
Position (kb) 362
363
364
365
366 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 361.461 kb on + strand, within lacY at 361.467 kb on + strand, within lacY at 361.467 kb on + strand, within lacY at 361.468 kb on - strand, within lacY at 361.485 kb on - strand, within lacY at 361.485 kb on - strand, within lacY at 361.489 kb on + strand, within lacY at 361.500 kb on + strand, within lacY at 361.500 kb on + strand, within lacY at 361.509 kb on + strand, within lacY at 361.509 kb on + strand, within lacY at 361.512 kb on - strand, within lacY at 361.514 kb on + strand, within lacY at 361.514 kb on - strand, within lacY at 361.517 kb on - strand, within lacY at 361.528 kb on - strand, within lacY at 361.544 kb on + strand, within lacY at 361.581 kb on - strand, within lacY at 361.581 kb on - strand, within lacY at 361.591 kb on + strand, within lacY at 361.605 kb on + strand, within lacY at 361.607 kb on + strand, within lacY at 361.607 kb on + strand, within lacY at 361.628 kb on + strand, within lacY at 361.634 kb on + strand, within lacY at 361.636 kb on + strand, within lacY at 361.636 kb on + strand, within lacY at 361.636 kb on + strand, within lacY at 361.638 kb on + strand, within lacY at 361.643 kb on - strand, within lacY at 361.688 kb on - strand, within lacY at 361.715 kb on + strand, within lacY at 361.723 kb on - strand, within lacY at 361.756 kb on - strand, within lacY at 361.759 kb on - strand, within lacY at 361.767 kb on - strand, within lacY at 361.767 kb on - strand, within lacY at 361.792 kb on + strand, within lacY at 361.800 kb on - strand, within lacY at 361.803 kb on - strand, within lacY at 361.818 kb on + strand, within lacY at 361.860 kb on - strand, within lacY at 361.867 kb on + strand, within lacY at 361.872 kb on + strand, within lacY at 361.872 kb on + strand, within lacY at 361.872 kb on + strand, within lacY at 361.879 kb on + strand, within lacY at 361.879 kb on + strand, within lacY at 361.879 kb on + strand, within lacY at 361.922 kb on - strand, within lacY at 361.924 kb on + strand, within lacY at 361.924 kb on + strand, within lacY at 361.924 kb on - strand, within lacY at 361.934 kb on + strand, within lacY at 361.935 kb on + strand, within lacY at 361.935 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.959 kb on - strand, within lacY at 361.961 kb on + strand, within lacY at 361.961 kb on - strand, within lacY at 361.963 kb on + strand, within lacY at 362.025 kb on + strand, within lacY at 362.026 kb on + strand, within lacY at 362.026 kb on + strand, within lacY at 362.026 kb on + strand, within lacY at 362.031 kb on + strand, within lacY at 362.031 kb on + strand, within lacY at 362.033 kb on - strand, within lacY at 362.069 kb on + strand, within lacY at 362.069 kb on + strand, within lacY at 362.088 kb on + strand, within lacY at 362.088 kb on + strand, within lacY at 362.089 kb on + strand, within lacY at 362.089 kb on + strand, within lacY at 362.151 kb on + strand, within lacY at 362.151 kb on + strand, within lacY at 362.163 kb on + strand, within lacY at 362.190 kb on + strand, within lacY at 362.284 kb on + strand at 362.289 kb on + strand at 362.289 kb on + strand at 362.289 kb on + strand at 362.289 kb on + strand at 362.297 kb on - strand at 362.406 kb on - strand at 362.406 kb on - strand at 362.432 kb on - strand at 362.432 kb on - strand at 362.433 kb on + strand at 362.433 kb on + strand at 362.441 kb on - strand at 362.478 kb on - strand at 362.478 kb on - strand at 362.484 kb on - strand at 362.486 kb on - strand at 362.486 kb on - strand at 362.498 kb on - strand at 362.498 kb on - strand at 362.554 kb on - strand at 362.557 kb on + strand at 362.564 kb on - strand at 362.587 kb on - strand at 362.587 kb on - strand at 362.616 kb on - strand at 362.622 kb on + strand at 362.659 kb on - strand at 362.659 kb on - strand at 362.687 kb on - strand at 362.708 kb on + strand at 362.716 kb on - strand at 362.823 kb on + strand, within lacZ at 362.945 kb on - strand, within lacZ at 363.014 kb on - strand, within lacZ at 363.027 kb on + strand, within lacZ at 363.121 kb on - strand, within lacZ at 363.121 kb on - strand, within lacZ at 363.157 kb on - strand, within lacZ at 363.157 kb on - strand, within lacZ at 363.184 kb on + strand, within lacZ at 363.380 kb on + strand, within lacZ at 363.388 kb on - strand, within lacZ at 363.445 kb on + strand, within lacZ at 363.447 kb on - strand, within lacZ at 363.576 kb on - strand, within lacZ at 365.812 kb on + strand, within lacI at 365.893 kb on - strand, within lacI at 365.913 kb on + strand, within lacI at 365.919 kb on + strand, within lacI at 365.959 kb on - strand, within lacI at 365.968 kb on - strand, within lacI at 365.984 kb on + strand, within lacI at 365.987 kb on - strand, within lacI at 365.987 kb on - strand, within lacI at 366.016 kb on + strand, within lacI at 366.040 kb on - strand, within lacI at 366.040 kb on - strand, within lacI at 366.265 kb on - strand, within lacI at 366.279 kb on + strand, within lacI at 366.339 kb on - strand, within lacI at 366.339 kb on - strand, within lacI at 366.369 kb on - strand, within lacI at 366.412 kb on + strand, within lacI at 366.415 kb on - strand, within lacI at 366.455 kb on + strand, within lacI
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 remove 361,461 + lacY b0343 0.25 -0.8 361,467 + lacY b0343 0.25 -0.6 361,467 + lacY b0343 0.25 +1.3 361,468 - lacY b0343 0.25 -0.8 361,485 - lacY b0343 0.27 -0.1 361,485 - lacY b0343 0.27 -0.1 361,489 + lacY b0343 0.27 +0.2 361,500 + lacY b0343 0.28 +0.7 361,500 + lacY b0343 0.28 -0.2 361,509 + lacY b0343 0.29 +1.0 361,509 + lacY b0343 0.29 -2.7 361,512 - lacY b0343 0.29 -0.1 361,514 + lacY b0343 0.29 +0.4 361,514 - lacY b0343 0.29 +0.7 361,517 - lacY b0343 0.29 -0.6 361,528 - lacY b0343 0.30 +0.1 361,544 + lacY b0343 0.31 -0.6 361,581 - lacY b0343 0.34 +0.3 361,581 - lacY b0343 0.34 +0.9 361,591 + lacY b0343 0.35 -0.3 361,605 + lacY b0343 0.36 -0.7 361,607 + lacY b0343 0.36 -0.4 361,607 + lacY b0343 0.36 -1.5 361,628 + lacY b0343 0.38 -0.5 361,634 + lacY b0343 0.39 -1.5 361,636 + lacY b0343 0.39 +0.8 361,636 + lacY b0343 0.39 +2.0 361,636 + lacY b0343 0.39 -2.4 361,638 + lacY b0343 0.39 -0.2 361,643 - lacY b0343 0.39 +0.3 361,688 - lacY b0343 0.43 -0.6 361,715 + lacY b0343 0.45 -0.5 361,723 - lacY b0343 0.46 -0.5 361,756 - lacY b0343 0.48 -0.1 361,759 - lacY b0343 0.49 -0.7 361,767 - lacY b0343 0.49 +0.5 361,767 - lacY b0343 0.49 -0.4 361,792 + lacY b0343 0.51 -0.5 361,800 - lacY b0343 0.52 -0.4 361,803 - lacY b0343 0.52 +0.0 361,818 + lacY b0343 0.53 -0.6 361,860 - lacY b0343 0.57 -0.0 361,867 + lacY b0343 0.57 -1.1 361,872 + lacY b0343 0.58 +0.3 361,872 + lacY b0343 0.58 -1.2 361,872 + lacY b0343 0.58 +0.1 361,879 + lacY b0343 0.58 +0.2 361,879 + lacY b0343 0.58 +1.3 361,879 + lacY b0343 0.58 -0.7 361,922 - lacY b0343 0.62 -1.4 361,924 + lacY b0343 0.62 -1.5 361,924 + lacY b0343 0.62 +0.3 361,924 - lacY b0343 0.62 -2.0 361,934 + lacY b0343 0.63 +0.9 361,935 + lacY b0343 0.63 -0.4 361,935 + lacY b0343 0.63 -0.3 361,953 + lacY b0343 0.64 -0.8 361,953 + lacY b0343 0.64 -0.6 361,953 + lacY b0343 0.64 +0.9 361,953 + lacY b0343 0.64 +0.4 361,953 + lacY b0343 0.64 +0.3 361,959 - lacY b0343 0.65 +1.8 361,961 + lacY b0343 0.65 -0.5 361,961 - lacY b0343 0.65 +1.8 361,963 + lacY b0343 0.65 +0.1 362,025 + lacY b0343 0.70 +0.3 362,026 + lacY b0343 0.70 -0.1 362,026 + lacY b0343 0.70 +0.4 362,026 + lacY b0343 0.70 -1.4 362,031 + lacY b0343 0.70 +0.5 362,031 + lacY b0343 0.70 -0.2 362,033 - lacY b0343 0.70 +1.8 362,069 + lacY b0343 0.73 -0.1 362,069 + lacY b0343 0.73 +0.1 362,088 + lacY b0343 0.75 +0.5 362,088 + lacY b0343 0.75 -0.9 362,089 + lacY b0343 0.75 +1.4 362,089 + lacY b0343 0.75 -1.6 362,151 + lacY b0343 0.80 -0.2 362,151 + lacY b0343 0.80 +0.4 362,163 + lacY b0343 0.81 +0.8 362,190 + lacY b0343 0.83 -0.7 362,284 + -0.3 362,289 + -0.4 362,289 + -0.1 362,289 + +1.0 362,289 + -0.3 362,297 - -3.1 362,406 - -1.2 362,406 - -0.7 362,432 - -1.2 362,432 - -1.2 362,433 + +2.0 362,433 + +3.3 362,441 - -0.8 362,478 - +0.8 362,478 - +0.8 362,484 - -0.1 362,486 - +0.3 362,486 - -0.4 362,498 - +0.4 362,498 - -0.2 362,554 - -1.5 362,557 + -0.8 362,564 - -0.9 362,587 - -0.3 362,587 - -1.1 362,616 - +0.2 362,622 + +0.6 362,659 - +0.4 362,659 - +0.3 362,687 - +0.2 362,708 + +0.2 362,716 - -0.1 362,823 + lacZ b0344 0.12 -0.1 362,945 - lacZ b0344 0.16 +0.1 363,014 - lacZ b0344 0.18 -0.0 363,027 + lacZ b0344 0.19 -0.5 363,121 - lacZ b0344 0.22 -0.5 363,121 - lacZ b0344 0.22 +0.1 363,157 - lacZ b0344 0.23 +0.2 363,157 - lacZ b0344 0.23 -0.0 363,184 + lacZ b0344 0.24 -0.3 363,380 + lacZ b0344 0.30 -0.2 363,388 - lacZ b0344 0.30 -0.3 363,445 + lacZ b0344 0.32 +0.1 363,447 - lacZ b0344 0.32 -0.0 363,576 - lacZ b0344 0.36 -0.4 365,812 + lacI b0345 0.15 -0.0 365,893 - lacI b0345 0.22 -0.4 365,913 + lacI b0345 0.24 -0.1 365,919 + lacI b0345 0.25 -0.5 365,959 - lacI b0345 0.28 -0.1 365,968 - lacI b0345 0.29 +0.2 365,984 + lacI b0345 0.31 -0.6 365,987 - lacI b0345 0.31 -0.4 365,987 - lacI b0345 0.31 -0.0 366,016 + lacI b0345 0.34 -0.0 366,040 - lacI b0345 0.36 +0.2 366,040 - lacI b0345 0.36 -0.4 366,265 - lacI b0345 0.57 -0.2 366,279 + lacI b0345 0.58 -1.3 366,339 - lacI b0345 0.63 +0.2 366,339 - lacI b0345 0.63 -0.3 366,369 - lacI b0345 0.66 -0.1 366,412 + lacI b0345 0.70 -0.3 366,415 - lacI b0345 0.70 +0.1 366,455 + lacI b0345 0.74 +0.1
Or see this region's nucleotide sequence