Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cynX and lacA are separated by 102 nucleotides lacA and lacY are separated by 65 nucleotides
b0341: cynX - predicted cyanate transporter (NCBI), at 359,216 to 360,370
cynX
b0342: lacA - galactoside O-acetyltransferase (NCBI), at 360,473 to 361,084
lacA
b0343: lacY - galactoside permease (NCBI), at 361,150 to 362,403
lacY
Position (kb) 360
361
362 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 359.491 kb on - strand, within cynX at 359.593 kb on + strand, within cynX at 359.593 kb on + strand, within cynX at 359.604 kb on - strand, within cynX at 359.606 kb on + strand, within cynX at 359.696 kb on - strand, within cynX at 359.785 kb on - strand, within cynX at 359.792 kb on + strand, within cynX at 359.839 kb on - strand, within cynX at 359.839 kb on - strand, within cynX at 359.916 kb on + strand, within cynX at 359.916 kb on + strand, within cynX at 359.943 kb on + strand, within cynX at 360.002 kb on + strand at 360.002 kb on + strand, within cynX at 360.010 kb on - strand, within cynX at 360.096 kb on + strand, within cynX at 360.096 kb on + strand, within cynX at 360.104 kb on - strand, within cynX at 360.109 kb on - strand, within cynX at 360.216 kb on + strand, within cynX at 360.244 kb on + strand, within cynX at 360.302 kb on + strand at 360.349 kb on + strand at 360.349 kb on + strand at 360.352 kb on + strand at 360.362 kb on - strand at 360.375 kb on + strand at 360.394 kb on + strand at 360.422 kb on - strand at 360.430 kb on + strand at 360.435 kb on + strand at 360.437 kb on + strand at 360.437 kb on + strand at 360.440 kb on + strand at 360.448 kb on + strand at 360.452 kb on + strand at 360.452 kb on + strand at 360.543 kb on + strand, within lacA at 360.581 kb on + strand, within lacA at 360.586 kb on + strand, within lacA at 360.586 kb on + strand, within lacA at 360.600 kb on - strand, within lacA at 360.600 kb on - strand, within lacA at 360.651 kb on - strand, within lacA at 360.731 kb on + strand, within lacA at 360.733 kb on + strand, within lacA at 360.733 kb on + strand, within lacA at 360.733 kb on + strand, within lacA at 360.733 kb on + strand, within lacA at 360.738 kb on - strand, within lacA at 360.739 kb on - strand, within lacA at 360.739 kb on - strand, within lacA at 360.739 kb on - strand, within lacA at 360.739 kb on - strand, within lacA at 360.739 kb on - strand, within lacA at 360.739 kb on - strand, within lacA at 360.740 kb on - strand, within lacA at 360.740 kb on - strand, within lacA at 360.740 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.741 kb on - strand, within lacA at 360.744 kb on + strand, within lacA at 360.744 kb on + strand, within lacA at 360.746 kb on - strand, within lacA at 360.746 kb on - strand, within lacA at 360.748 kb on - strand, within lacA at 360.748 kb on - strand, within lacA at 360.753 kb on - strand, within lacA at 360.776 kb on + strand, within lacA at 360.778 kb on + strand, within lacA at 360.791 kb on + strand, within lacA at 360.791 kb on + strand, within lacA at 360.798 kb on + strand, within lacA at 360.798 kb on + strand, within lacA at 360.799 kb on - strand, within lacA at 360.806 kb on + strand, within lacA at 360.806 kb on + strand, within lacA at 360.816 kb on - strand, within lacA at 360.816 kb on - strand, within lacA at 360.816 kb on - strand, within lacA at 360.816 kb on - strand, within lacA at 360.850 kb on + strand, within lacA at 360.850 kb on + strand, within lacA at 360.852 kb on + strand, within lacA at 360.852 kb on + strand, within lacA at 360.852 kb on + strand, within lacA at 360.852 kb on + strand, within lacA at 360.852 kb on + strand, within lacA at 360.869 kb on - strand, within lacA at 360.875 kb on - strand, within lacA at 360.896 kb on - strand, within lacA at 360.897 kb on - strand, within lacA at 360.905 kb on + strand, within lacA at 360.905 kb on + strand, within lacA at 360.959 kb on + strand, within lacA at 360.963 kb on + strand, within lacA at 360.963 kb on + strand, within lacA at 360.965 kb on + strand, within lacA at 360.965 kb on + strand, within lacA at 360.967 kb on - strand, within lacA at 361.024 kb on - strand at 361.031 kb on - strand at 361.033 kb on - strand at 361.035 kb on + strand at 361.062 kb on - strand at 361.074 kb on + strand at 361.074 kb on + strand at 361.076 kb on - strand at 361.079 kb on + strand at 361.087 kb on - strand at 361.087 kb on - strand at 361.087 kb on - strand at 361.087 kb on - strand at 361.093 kb on + strand at 361.094 kb on - strand at 361.094 kb on - strand at 361.100 kb on + strand at 361.100 kb on + strand at 361.100 kb on + strand at 361.100 kb on - strand at 361.105 kb on - strand at 361.107 kb on + strand at 361.108 kb on - strand at 361.115 kb on + strand at 361.138 kb on + strand at 361.138 kb on + strand at 361.149 kb on - strand at 361.151 kb on - strand at 361.179 kb on - strand at 361.186 kb on + strand at 361.239 kb on - strand at 361.239 kb on - strand at 361.244 kb on - strand at 361.244 kb on - strand at 361.246 kb on + strand at 361.270 kb on - strand at 361.288 kb on + strand, within lacY at 361.288 kb on + strand, within lacY at 361.300 kb on - strand, within lacY at 361.302 kb on + strand, within lacY at 361.307 kb on - strand, within lacY at 361.315 kb on - strand, within lacY at 361.336 kb on + strand, within lacY at 361.360 kb on - strand, within lacY at 361.377 kb on + strand, within lacY at 361.409 kb on + strand, within lacY at 361.413 kb on + strand, within lacY at 361.419 kb on - strand, within lacY at 361.419 kb on - strand, within lacY at 361.419 kb on - strand, within lacY at 361.420 kb on - strand, within lacY at 361.420 kb on - strand, within lacY at 361.433 kb on + strand, within lacY at 361.441 kb on - strand, within lacY at 361.441 kb on - strand, within lacY at 361.452 kb on + strand, within lacY at 361.461 kb on + strand, within lacY at 361.467 kb on + strand, within lacY at 361.467 kb on + strand, within lacY at 361.468 kb on - strand, within lacY at 361.485 kb on - strand, within lacY at 361.485 kb on - strand, within lacY at 361.489 kb on + strand, within lacY at 361.500 kb on + strand, within lacY at 361.500 kb on + strand, within lacY at 361.509 kb on + strand, within lacY at 361.509 kb on + strand, within lacY at 361.512 kb on - strand, within lacY at 361.514 kb on + strand, within lacY at 361.514 kb on - strand, within lacY at 361.517 kb on - strand, within lacY at 361.528 kb on - strand, within lacY at 361.544 kb on + strand, within lacY at 361.581 kb on - strand, within lacY at 361.581 kb on - strand, within lacY at 361.591 kb on + strand, within lacY at 361.605 kb on + strand, within lacY at 361.607 kb on + strand, within lacY at 361.607 kb on + strand, within lacY at 361.628 kb on + strand, within lacY at 361.634 kb on + strand, within lacY at 361.636 kb on + strand, within lacY at 361.636 kb on + strand, within lacY at 361.636 kb on + strand, within lacY at 361.638 kb on + strand, within lacY at 361.643 kb on - strand, within lacY at 361.688 kb on - strand, within lacY at 361.715 kb on + strand, within lacY at 361.723 kb on - strand, within lacY at 361.756 kb on - strand, within lacY at 361.759 kb on - strand, within lacY at 361.767 kb on - strand, within lacY at 361.767 kb on - strand, within lacY at 361.792 kb on + strand, within lacY at 361.800 kb on - strand, within lacY at 361.803 kb on - strand, within lacY at 361.818 kb on + strand, within lacY at 361.860 kb on - strand, within lacY at 361.867 kb on + strand, within lacY at 361.872 kb on + strand, within lacY at 361.872 kb on + strand, within lacY at 361.872 kb on + strand, within lacY at 361.879 kb on + strand, within lacY at 361.879 kb on + strand, within lacY at 361.879 kb on + strand, within lacY at 361.922 kb on - strand, within lacY at 361.924 kb on + strand, within lacY at 361.924 kb on + strand, within lacY at 361.924 kb on - strand, within lacY at 361.934 kb on + strand, within lacY at 361.935 kb on + strand, within lacY at 361.935 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.953 kb on + strand, within lacY at 361.959 kb on - strand, within lacY at 361.961 kb on + strand, within lacY at 361.961 kb on - strand, within lacY at 361.963 kb on + strand, within lacY at 362.025 kb on + strand, within lacY at 362.026 kb on + strand, within lacY at 362.026 kb on + strand, within lacY at 362.026 kb on + strand, within lacY at 362.031 kb on + strand, within lacY at 362.031 kb on + strand, within lacY at 362.033 kb on - strand, within lacY at 362.069 kb on + strand, within lacY at 362.069 kb on + strand, within lacY
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 remove 359,491 - cynX b0341 0.24 -1.8 359,593 + cynX b0341 0.33 -0.9 359,593 + cynX b0341 0.33 -0.5 359,604 - cynX b0341 0.34 +0.1 359,606 + cynX b0341 0.34 +0.2 359,696 - cynX b0341 0.42 -0.1 359,785 - cynX b0341 0.49 -0.4 359,792 + cynX b0341 0.50 +0.2 359,839 - cynX b0341 0.54 -0.0 359,839 - cynX b0341 0.54 -0.1 359,916 + cynX b0341 0.61 -1.0 359,916 + cynX b0341 0.61 +0.5 359,943 + cynX b0341 0.63 -0.2 360,002 + -1.9 360,002 + cynX b0341 0.68 +0.6 360,010 - cynX b0341 0.69 -0.5 360,096 + cynX b0341 0.76 -0.1 360,096 + cynX b0341 0.76 -0.4 360,104 - cynX b0341 0.77 -0.3 360,109 - cynX b0341 0.77 -0.6 360,216 + cynX b0341 0.87 +0.4 360,244 + cynX b0341 0.89 +0.0 360,302 + +0.1 360,349 + -0.2 360,349 + +0.1 360,352 + -0.3 360,362 - +1.5 360,375 + +0.1 360,394 + +0.8 360,422 - -1.7 360,430 + -0.2 360,435 + -0.3 360,437 + -1.4 360,437 + -0.3 360,440 + +0.2 360,448 + -0.2 360,452 + +0.6 360,452 + +0.0 360,543 + lacA b0342 0.11 -1.0 360,581 + lacA b0342 0.18 -1.1 360,586 + lacA b0342 0.18 +1.8 360,586 + lacA b0342 0.18 -1.2 360,600 - lacA b0342 0.21 -1.3 360,600 - lacA b0342 0.21 -1.2 360,651 - lacA b0342 0.29 -0.8 360,731 + lacA b0342 0.42 -0.0 360,733 + lacA b0342 0.42 -0.4 360,733 + lacA b0342 0.42 -0.6 360,733 + lacA b0342 0.42 -0.2 360,733 + lacA b0342 0.42 +0.2 360,738 - lacA b0342 0.43 -0.6 360,739 - lacA b0342 0.43 +0.2 360,739 - lacA b0342 0.43 -1.1 360,739 - lacA b0342 0.43 +1.3 360,739 - lacA b0342 0.43 -0.2 360,739 - lacA b0342 0.43 -0.2 360,739 - lacA b0342 0.43 +1.4 360,740 - lacA b0342 0.44 -0.6 360,740 - lacA b0342 0.44 -0.3 360,740 - lacA b0342 0.44 +0.2 360,741 - lacA b0342 0.44 +0.0 360,741 - lacA b0342 0.44 -0.0 360,741 - lacA b0342 0.44 -0.8 360,741 - lacA b0342 0.44 -0.3 360,741 - lacA b0342 0.44 -0.3 360,741 - lacA b0342 0.44 +0.4 360,741 - lacA b0342 0.44 +0.2 360,744 + lacA b0342 0.44 -1.4 360,744 + lacA b0342 0.44 -0.9 360,746 - lacA b0342 0.45 +0.1 360,746 - lacA b0342 0.45 -0.3 360,748 - lacA b0342 0.45 +0.0 360,748 - lacA b0342 0.45 -1.0 360,753 - lacA b0342 0.46 +4.4 360,776 + lacA b0342 0.50 -0.1 360,778 + lacA b0342 0.50 -1.6 360,791 + lacA b0342 0.52 -1.7 360,791 + lacA b0342 0.52 +0.3 360,798 + lacA b0342 0.53 -1.4 360,798 + lacA b0342 0.53 +1.4 360,799 - lacA b0342 0.53 -0.9 360,806 + lacA b0342 0.54 -0.4 360,806 + lacA b0342 0.54 +0.4 360,816 - lacA b0342 0.56 -1.7 360,816 - lacA b0342 0.56 -0.2 360,816 - lacA b0342 0.56 -1.8 360,816 - lacA b0342 0.56 -0.5 360,850 + lacA b0342 0.62 +0.2 360,850 + lacA b0342 0.62 -1.4 360,852 + lacA b0342 0.62 +0.1 360,852 + lacA b0342 0.62 -0.7 360,852 + lacA b0342 0.62 -0.0 360,852 + lacA b0342 0.62 -1.4 360,852 + lacA b0342 0.62 -0.6 360,869 - lacA b0342 0.65 -1.0 360,875 - lacA b0342 0.66 +0.5 360,896 - lacA b0342 0.69 +2.8 360,897 - lacA b0342 0.69 +0.1 360,905 + lacA b0342 0.71 -0.6 360,905 + lacA b0342 0.71 -0.1 360,959 + lacA b0342 0.79 +0.7 360,963 + lacA b0342 0.80 +0.2 360,963 + lacA b0342 0.80 -0.2 360,965 + lacA b0342 0.80 -0.0 360,965 + lacA b0342 0.80 -1.4 360,967 - lacA b0342 0.81 -0.8 361,024 - -0.4 361,031 - -0.3 361,033 - -0.7 361,035 + -1.2 361,062 - -0.0 361,074 + -0.4 361,074 + +0.2 361,076 - -0.5 361,079 + +1.8 361,087 - -1.0 361,087 - -0.1 361,087 - -0.6 361,087 - +0.1 361,093 + -0.7 361,094 - -0.6 361,094 - -0.4 361,100 + +0.9 361,100 + +0.1 361,100 + +0.1 361,100 - +4.3 361,105 - -0.0 361,107 + -0.8 361,108 - -0.0 361,115 + -0.6 361,138 + -0.1 361,138 + +1.5 361,149 - -0.9 361,151 - -1.0 361,179 - -0.5 361,186 + -0.2 361,239 - -2.0 361,239 - -0.0 361,244 - +0.3 361,244 - -1.2 361,246 + -1.6 361,270 - -1.5 361,288 + lacY b0343 0.11 +0.6 361,288 + lacY b0343 0.11 -0.4 361,300 - lacY b0343 0.12 -0.1 361,302 + lacY b0343 0.12 -1.5 361,307 - lacY b0343 0.13 +0.5 361,315 - lacY b0343 0.13 -0.6 361,336 + lacY b0343 0.15 -0.4 361,360 - lacY b0343 0.17 -0.4 361,377 + lacY b0343 0.18 +0.1 361,409 + lacY b0343 0.21 +0.3 361,413 + lacY b0343 0.21 +0.5 361,419 - lacY b0343 0.21 -0.7 361,419 - lacY b0343 0.21 -0.5 361,419 - lacY b0343 0.21 -1.7 361,420 - lacY b0343 0.22 +0.5 361,420 - lacY b0343 0.22 -0.6 361,433 + lacY b0343 0.23 +0.1 361,441 - lacY b0343 0.23 +0.9 361,441 - lacY b0343 0.23 -0.7 361,452 + lacY b0343 0.24 -0.2 361,461 + lacY b0343 0.25 -0.8 361,467 + lacY b0343 0.25 -0.6 361,467 + lacY b0343 0.25 +1.3 361,468 - lacY b0343 0.25 -0.8 361,485 - lacY b0343 0.27 -0.1 361,485 - lacY b0343 0.27 -0.1 361,489 + lacY b0343 0.27 +0.2 361,500 + lacY b0343 0.28 +0.7 361,500 + lacY b0343 0.28 -0.2 361,509 + lacY b0343 0.29 +1.0 361,509 + lacY b0343 0.29 -2.7 361,512 - lacY b0343 0.29 -0.1 361,514 + lacY b0343 0.29 +0.4 361,514 - lacY b0343 0.29 +0.7 361,517 - lacY b0343 0.29 -0.6 361,528 - lacY b0343 0.30 +0.1 361,544 + lacY b0343 0.31 -0.6 361,581 - lacY b0343 0.34 +0.3 361,581 - lacY b0343 0.34 +0.9 361,591 + lacY b0343 0.35 -0.3 361,605 + lacY b0343 0.36 -0.7 361,607 + lacY b0343 0.36 -0.4 361,607 + lacY b0343 0.36 -1.5 361,628 + lacY b0343 0.38 -0.5 361,634 + lacY b0343 0.39 -1.5 361,636 + lacY b0343 0.39 +0.8 361,636 + lacY b0343 0.39 +2.0 361,636 + lacY b0343 0.39 -2.4 361,638 + lacY b0343 0.39 -0.2 361,643 - lacY b0343 0.39 +0.3 361,688 - lacY b0343 0.43 -0.6 361,715 + lacY b0343 0.45 -0.5 361,723 - lacY b0343 0.46 -0.5 361,756 - lacY b0343 0.48 -0.1 361,759 - lacY b0343 0.49 -0.7 361,767 - lacY b0343 0.49 +0.5 361,767 - lacY b0343 0.49 -0.4 361,792 + lacY b0343 0.51 -0.5 361,800 - lacY b0343 0.52 -0.4 361,803 - lacY b0343 0.52 +0.0 361,818 + lacY b0343 0.53 -0.6 361,860 - lacY b0343 0.57 -0.0 361,867 + lacY b0343 0.57 -1.1 361,872 + lacY b0343 0.58 +0.3 361,872 + lacY b0343 0.58 -1.2 361,872 + lacY b0343 0.58 +0.1 361,879 + lacY b0343 0.58 +0.2 361,879 + lacY b0343 0.58 +1.3 361,879 + lacY b0343 0.58 -0.7 361,922 - lacY b0343 0.62 -1.4 361,924 + lacY b0343 0.62 -1.5 361,924 + lacY b0343 0.62 +0.3 361,924 - lacY b0343 0.62 -2.0 361,934 + lacY b0343 0.63 +0.9 361,935 + lacY b0343 0.63 -0.4 361,935 + lacY b0343 0.63 -0.3 361,953 + lacY b0343 0.64 -0.8 361,953 + lacY b0343 0.64 -0.6 361,953 + lacY b0343 0.64 +0.9 361,953 + lacY b0343 0.64 +0.4 361,953 + lacY b0343 0.64 +0.3 361,959 - lacY b0343 0.65 +1.8 361,961 + lacY b0343 0.65 -0.5 361,961 - lacY b0343 0.65 +1.8 361,963 + lacY b0343 0.65 +0.1 362,025 + lacY b0343 0.70 +0.3 362,026 + lacY b0343 0.70 -0.1 362,026 + lacY b0343 0.70 +0.4 362,026 + lacY b0343 0.70 -1.4 362,031 + lacY b0343 0.70 +0.5 362,031 + lacY b0343 0.70 -0.2 362,033 - lacY b0343 0.70 +1.8 362,069 + lacY b0343 0.73 -0.1 362,069 + lacY b0343 0.73 +0.1
Or see this region's nucleotide sequence