Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tilS and yaeO are separated by 48 nucleotides yaeO and yaeP overlap by 8 nucleotides yaeP and yaeQ are separated by 165 nucleotides yaeQ and yaeJ overlap by 4 nucleotides
b0188: tilS - tRNA(Ile)-lysidine synthetase (NCBI), at 212,331 to 213,629
tilS
b0189: yaeO - orf, hypothetical protein (VIMSS), at 213,678 to 213,932
yaeO
b4406: yaeP - hypothetical protein (NCBI), at 213,925 to 214,125
yaeP
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
Position (kb) 213
214 Strain fitness (log2 ratio) -1
0
1 at 213.686 kb on + strand at 213.686 kb on + strand at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.783 kb on + strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.913 kb on + strand at 213.913 kb on + strand at 213.956 kb on - strand, within yaeP at 213.956 kb on - strand, within yaeP at 214.040 kb on - strand, within yaeP at 214.130 kb on + strand at 214.142 kb on + strand at 214.166 kb on + strand at 214.174 kb on - strand at 214.174 kb on - strand at 214.216 kb on - strand at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 remove 213,686 + -0.4 213,686 + -0.3 213,716 + yaeO b0189 0.15 +0.1 213,716 + yaeO b0189 0.15 -0.8 213,716 + yaeO b0189 0.15 -0.4 213,716 + yaeO b0189 0.15 -0.6 213,783 + yaeO b0189 0.41 -0.5 213,880 - yaeO b0189 0.79 -0.7 213,880 - yaeO b0189 0.79 +0.0 213,913 + +0.7 213,913 + -0.5 213,956 - yaeP b4406 0.15 -0.3 213,956 - yaeP b4406 0.15 -0.6 214,040 - yaeP b4406 0.57 -0.2 214,130 + -0.4 214,142 + -0.1 214,166 + +0.5 214,174 - -0.8 214,174 - +0.1 214,216 - +0.1 214,369 - yaeQ b0190 0.14 -0.4 214,391 - yaeQ b0190 0.18 -0.3 214,404 - yaeQ b0190 0.21 -0.1 214,419 - yaeQ b0190 0.23 +0.1 214,468 + yaeQ b0190 0.32 +0.7 214,487 + yaeQ b0190 0.36 +0.3 214,487 + yaeQ b0190 0.36 -0.7 214,542 + yaeQ b0190 0.46 +0.7 214,572 + yaeQ b0190 0.51 +0.7 214,574 + yaeQ b0190 0.52 +0.9 214,600 - yaeQ b0190 0.57 -0.6 214,606 + yaeQ b0190 0.58 +0.1 214,606 + yaeQ b0190 0.58 +1.1 214,764 - yaeQ b0190 0.87 -0.7 214,773 - yaeQ b0190 0.88 -0.2 214,808 + +1.6 214,825 - -0.2 214,836 - +0.6 214,872 + +1.4 214,874 + +1.1 214,919 + yaeJ b0191 0.20 +1.4
Or see this region's nucleotide sequence