Strain Fitness in Escherichia coli BW25113 around b2221

Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
2,320,600	+	atoC	b2220	0.51	-0.7
2,320,640	+	atoC	b2220	0.54	+1.3
2,320,641	+	atoC	b2220	0.54	-0.1
2,320,668	+	atoC	b2220	0.56	-1.0
2,320,668	+	atoC	b2220	0.56	+0.0
2,320,671	-	atoC	b2220	0.56	-1.2
2,320,674	-	atoC	b2220	0.57	-0.2
2,320,752	+	atoC	b2220	0.62	+0.9
2,320,752	+	atoC	b2220	0.62	+0.5
2,320,833	-	atoC	b2220	0.68	-0.8
2,320,833	-	atoC	b2220	0.68	+0.0
2,320,936	+	atoC	b2220	0.76	+0.3
2,320,936	+	atoC	b2220	0.76	+0.1
2,320,938	+	atoC	b2220	0.76	-0.3
2,320,944	-	atoC	b2220	0.76	+0.2
2,320,944	-	atoC	b2220	0.76	-0.2
2,320,972	+	atoC	b2220	0.78	+0.3
2,320,972	+	atoC	b2220	0.78	+0.1
2,321,007	+	atoC	b2220	0.81	-0.6
2,321,014	+	atoC	b2220	0.81	+0.2
2,321,179	-				-0.8
2,321,194	-				+1.4
2,321,196	+				+0.6
2,321,196	+				-0.8
2,321,236	-				-0.4
2,321,261	+				+2.1
2,321,266	+				+0.1
2,321,496	-				-0.6
2,321,519	-				+0.3
2,321,700	+	atoD	b2221	0.35	-0.3
2,321,707	-	atoD	b2221	0.36	+3.9
2,321,818	+	atoD	b2221	0.53	-0.1
2,321,967	+	atoD	b2221	0.75	+0.2
2,322,085	-				+0.9
2,322,255	-	atoA	b2222	0.19	-0.2
2,322,362	-	atoA	b2222	0.35	-0.5
2,322,391	+	atoA	b2222	0.40	-0.4
2,322,410	-	atoA	b2222	0.43	-0.4
2,322,423	-	atoA	b2222	0.45	-0.0
2,322,629	-	atoA	b2222	0.76	-1.1
2,322,892	+				+0.3
2,322,927	+	atoE	b2223	0.11	-0.7
2,322,978	-	atoE	b2223	0.15	-3.4
2,322,978	-	atoE	b2223	0.15	-1.0
2,322,999	+	atoE	b2223	0.17	-0.7
2,323,113	-	atoE	b2223	0.25	+0.1