Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt htrE and ecpD are separated by 34 nucleotides ecpD and yadN are separated by 97 nucleotides yadN and folK are separated by 369 nucleotides folK and pcnB overlap by 4 nucleotides
b0139: htrE - predicted outer membrane usher protein (NCBI), at 152,829 to 155,426
htrE
b0140: ecpD - predicted periplasmic pilin chaperone (NCBI), at 155,461 to 156,201
ecpD
b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883
yadN
b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732
folK
b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126
pcnB
Position (kb) 156
157 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 155.306 kb on + strand at 155.307 kb on + strand at 155.314 kb on + strand at 155.321 kb on - strand at 155.321 kb on - strand at 155.322 kb on - strand at 155.322 kb on - strand at 155.327 kb on - strand at 155.333 kb on + strand at 155.341 kb on - strand at 155.341 kb on - strand at 155.341 kb on - strand at 155.345 kb on + strand at 155.345 kb on + strand at 155.345 kb on + strand at 155.351 kb on - strand at 155.356 kb on + strand at 155.356 kb on + strand at 155.356 kb on + strand at 155.358 kb on - strand at 155.364 kb on - strand at 155.364 kb on - strand at 155.376 kb on - strand at 155.378 kb on + strand at 155.383 kb on + strand at 155.393 kb on + strand at 155.396 kb on - strand at 155.434 kb on - strand at 155.466 kb on + strand at 155.466 kb on + strand at 155.474 kb on - strand at 155.484 kb on - strand at 155.505 kb on - strand at 155.547 kb on - strand, within ecpD at 155.550 kb on - strand, within ecpD at 155.558 kb on + strand, within ecpD at 155.567 kb on - strand, within ecpD at 155.576 kb on - strand, within ecpD at 155.576 kb on - strand, within ecpD at 155.576 kb on - strand, within ecpD at 155.576 kb on - strand, within ecpD at 155.578 kb on - strand, within ecpD at 155.617 kb on - strand, within ecpD at 155.625 kb on - strand, within ecpD at 155.625 kb on - strand, within ecpD at 155.645 kb on - strand, within ecpD at 155.671 kb on - strand, within ecpD at 155.684 kb on + strand, within ecpD at 155.707 kb on + strand, within ecpD at 155.710 kb on - strand, within ecpD at 155.710 kb on - strand, within ecpD at 155.719 kb on + strand, within ecpD at 155.752 kb on - strand, within ecpD at 155.802 kb on - strand, within ecpD at 155.802 kb on - strand, within ecpD at 155.831 kb on - strand, within ecpD at 155.831 kb on - strand, within ecpD at 155.864 kb on - strand, within ecpD at 155.874 kb on - strand, within ecpD at 155.881 kb on + strand, within ecpD at 155.881 kb on + strand, within ecpD at 155.881 kb on + strand, within ecpD at 155.883 kb on - strand, within ecpD at 155.883 kb on - strand, within ecpD at 155.885 kb on - strand, within ecpD at 155.885 kb on - strand, within ecpD at 155.887 kb on + strand, within ecpD at 155.887 kb on + strand, within ecpD at 155.888 kb on + strand, within ecpD at 155.897 kb on + strand, within ecpD at 155.933 kb on + strand, within ecpD at 155.943 kb on + strand, within ecpD at 155.947 kb on + strand, within ecpD at 155.947 kb on + strand, within ecpD at 155.952 kb on + strand, within ecpD at 155.952 kb on + strand, within ecpD at 155.964 kb on + strand, within ecpD at 155.964 kb on + strand, within ecpD at 155.966 kb on + strand, within ecpD at 155.966 kb on + strand, within ecpD at 155.969 kb on + strand, within ecpD at 155.969 kb on + strand, within ecpD at 155.969 kb on - strand, within ecpD at 155.979 kb on + strand, within ecpD at 155.979 kb on + strand, within ecpD at 155.979 kb on + strand, within ecpD at 155.981 kb on + strand, within ecpD at 155.987 kb on + strand, within ecpD at 155.987 kb on + strand, within ecpD at 155.999 kb on + strand, within ecpD at 155.999 kb on + strand, within ecpD at 155.999 kb on + strand, within ecpD at 155.999 kb on + strand, within ecpD at 156.010 kb on + strand, within ecpD at 156.011 kb on + strand, within ecpD at 156.016 kb on + strand, within ecpD at 156.018 kb on - strand, within ecpD at 156.020 kb on + strand, within ecpD at 156.020 kb on + strand, within ecpD at 156.020 kb on + strand, within ecpD at 156.025 kb on + strand, within ecpD at 156.025 kb on + strand, within ecpD at 156.030 kb on + strand, within ecpD at 156.033 kb on - strand, within ecpD at 156.035 kb on + strand, within ecpD at 156.063 kb on + strand, within ecpD at 156.124 kb on + strand, within ecpD at 156.147 kb on + strand at 156.157 kb on + strand at 156.157 kb on + strand at 156.157 kb on + strand at 156.157 kb on + strand at 156.157 kb on + strand at 156.157 kb on + strand at 156.166 kb on + strand at 156.166 kb on + strand at 156.176 kb on + strand at 156.176 kb on + strand at 156.198 kb on + strand at 156.198 kb on + strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.312 kb on - strand at 156.322 kb on - strand at 156.322 kb on - strand at 156.322 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.332 kb on - strand at 156.332 kb on - strand at 156.356 kb on - strand at 156.356 kb on - strand at 156.356 kb on - strand at 156.356 kb on - strand at 156.359 kb on - strand, within yadN at 156.361 kb on - strand, within yadN at 156.367 kb on - strand, within yadN at 156.376 kb on - strand, within yadN at 156.376 kb on - strand, within yadN at 156.427 kb on - strand, within yadN at 156.428 kb on - strand, within yadN at 156.428 kb on - strand, within yadN at 156.429 kb on + strand, within yadN at 156.429 kb on + strand, within yadN at 156.431 kb on + strand, within yadN at 156.431 kb on + strand, within yadN at 156.432 kb on + strand, within yadN at 156.432 kb on + strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.440 kb on - strand, within yadN at 156.486 kb on + strand, within yadN at 156.489 kb on - strand, within yadN at 156.489 kb on - strand, within yadN at 156.521 kb on - strand, within yadN at 156.533 kb on - strand, within yadN at 156.545 kb on - strand, within yadN at 156.547 kb on - strand, within yadN at 156.560 kb on - strand, within yadN at 156.600 kb on + strand, within yadN at 156.602 kb on + strand, within yadN at 156.605 kb on - strand, within yadN at 156.642 kb on + strand, within yadN at 156.661 kb on + strand, within yadN at 156.682 kb on - strand, within yadN at 156.682 kb on - strand, within yadN at 156.685 kb on - strand, within yadN at 156.685 kb on - strand, within yadN at 156.685 kb on - strand, within yadN at 156.687 kb on - strand, within yadN at 156.705 kb on + strand, within yadN at 156.711 kb on + strand, within yadN at 156.721 kb on + strand, within yadN at 156.721 kb on + strand, within yadN at 156.721 kb on + strand, within yadN at 156.722 kb on + strand, within yadN at 156.734 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.744 kb on + strand, within yadN at 156.755 kb on + strand, within yadN at 156.757 kb on + strand, within yadN at 156.759 kb on + strand, within yadN at 156.786 kb on + strand, within yadN at 156.789 kb on + strand, within yadN at 156.793 kb on + strand, within yadN at 156.804 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.825 kb on + strand at 156.827 kb on + strand at 156.827 kb on + strand at 156.833 kb on - strand at 156.834 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.850 kb on + strand at 156.854 kb on + strand at 156.854 kb on + strand at 157.218 kb on - strand at 157.279 kb on - strand at 157.291 kb on - strand at 157.301 kb on - strand, within folK at 157.745 kb on + strand at 157.870 kb on - strand, within pcnB
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 remove 155,306 + -0.2 155,307 + +1.8 155,314 + +1.8 155,321 - -0.4 155,321 - +0.1 155,322 - -0.8 155,322 - -0.9 155,327 - +2.3 155,333 + +0.8 155,341 - +1.4 155,341 - -0.4 155,341 - +0.8 155,345 + +0.8 155,345 + -0.1 155,345 + +1.8 155,351 - -0.4 155,356 + +1.8 155,356 + +1.8 155,356 + +1.8 155,358 - +0.2 155,364 - +1.3 155,364 - +0.2 155,376 - -0.7 155,378 + +0.2 155,383 + +0.1 155,393 + +1.8 155,396 - -0.3 155,434 - -0.6 155,466 + +1.8 155,466 + +1.8 155,474 - -0.5 155,484 - -0.2 155,505 - -0.4 155,547 - ecpD b0140 0.12 +0.4 155,550 - ecpD b0140 0.12 +0.5 155,558 + ecpD b0140 0.13 -1.2 155,567 - ecpD b0140 0.14 -0.8 155,576 - ecpD b0140 0.16 -0.3 155,576 - ecpD b0140 0.16 -0.6 155,576 - ecpD b0140 0.16 +1.4 155,576 - ecpD b0140 0.16 -0.5 155,578 - ecpD b0140 0.16 +0.3 155,617 - ecpD b0140 0.21 -0.3 155,625 - ecpD b0140 0.22 -0.2 155,625 - ecpD b0140 0.22 -0.7 155,645 - ecpD b0140 0.25 -0.4 155,671 - ecpD b0140 0.28 -0.0 155,684 + ecpD b0140 0.30 +0.0 155,707 + ecpD b0140 0.33 -0.1 155,710 - ecpD b0140 0.34 -0.2 155,710 - ecpD b0140 0.34 +1.3 155,719 + ecpD b0140 0.35 -0.5 155,752 - ecpD b0140 0.39 -0.0 155,802 - ecpD b0140 0.46 +0.1 155,802 - ecpD b0140 0.46 -0.8 155,831 - ecpD b0140 0.50 +0.4 155,831 - ecpD b0140 0.50 +0.6 155,864 - ecpD b0140 0.54 +0.4 155,874 - ecpD b0140 0.56 -1.3 155,881 + ecpD b0140 0.57 -0.2 155,881 + ecpD b0140 0.57 -0.7 155,881 + ecpD b0140 0.57 +0.1 155,883 - ecpD b0140 0.57 -0.2 155,883 - ecpD b0140 0.57 +1.2 155,885 - ecpD b0140 0.57 -1.9 155,885 - ecpD b0140 0.57 +0.4 155,887 + ecpD b0140 0.57 -0.2 155,887 + ecpD b0140 0.57 -0.2 155,888 + ecpD b0140 0.58 -0.0 155,897 + ecpD b0140 0.59 -0.8 155,933 + ecpD b0140 0.64 +1.2 155,943 + ecpD b0140 0.65 -0.2 155,947 + ecpD b0140 0.66 -0.8 155,947 + ecpD b0140 0.66 +0.1 155,952 + ecpD b0140 0.66 +0.5 155,952 + ecpD b0140 0.66 -1.2 155,964 + ecpD b0140 0.68 -0.6 155,964 + ecpD b0140 0.68 +0.2 155,966 + ecpD b0140 0.68 -0.0 155,966 + ecpD b0140 0.68 -0.2 155,969 + ecpD b0140 0.69 +0.1 155,969 + ecpD b0140 0.69 +2.0 155,969 - ecpD b0140 0.69 +1.8 155,979 + ecpD b0140 0.70 +0.2 155,979 + ecpD b0140 0.70 -0.7 155,979 + ecpD b0140 0.70 -0.6 155,981 + ecpD b0140 0.70 +0.6 155,987 + ecpD b0140 0.71 +0.0 155,987 + ecpD b0140 0.71 -2.0 155,999 + ecpD b0140 0.73 +0.9 155,999 + ecpD b0140 0.73 -0.7 155,999 + ecpD b0140 0.73 -1.2 155,999 + ecpD b0140 0.73 -0.5 156,010 + ecpD b0140 0.74 +0.5 156,011 + ecpD b0140 0.74 -0.2 156,016 + ecpD b0140 0.75 -0.3 156,018 - ecpD b0140 0.75 -1.0 156,020 + ecpD b0140 0.75 +0.9 156,020 + ecpD b0140 0.75 +0.3 156,020 + ecpD b0140 0.75 -0.3 156,025 + ecpD b0140 0.76 -0.6 156,025 + ecpD b0140 0.76 +0.8 156,030 + ecpD b0140 0.77 +0.8 156,033 - ecpD b0140 0.77 +1.8 156,035 + ecpD b0140 0.77 +1.3 156,063 + ecpD b0140 0.81 +0.7 156,124 + ecpD b0140 0.89 -0.2 156,147 + +0.4 156,157 + +0.7 156,157 + +0.2 156,157 + -0.0 156,157 + +0.4 156,157 + +1.8 156,157 + -0.3 156,166 + -0.2 156,166 + -0.6 156,176 + -0.8 156,176 + -0.6 156,198 + +0.2 156,198 + -0.4 156,298 - -1.1 156,298 - -0.1 156,298 - -0.6 156,298 - +0.0 156,298 - +0.2 156,298 - -0.9 156,298 - +1.0 156,312 - -0.3 156,322 - +1.0 156,322 - +0.1 156,322 - -1.2 156,324 - -0.0 156,324 - -2.0 156,324 - +0.7 156,324 - +1.0 156,324 - -0.0 156,324 - +0.1 156,324 - -0.3 156,324 - -1.2 156,324 - +0.0 156,324 - +1.0 156,332 - -0.7 156,332 - -0.2 156,356 - +0.0 156,356 - +0.0 156,356 - -0.7 156,356 - +1.0 156,359 - yadN b0141 0.10 -0.5 156,361 - yadN b0141 0.11 +0.5 156,367 - yadN b0141 0.12 +0.5 156,376 - yadN b0141 0.13 +0.2 156,376 - yadN b0141 0.13 +0.6 156,427 - yadN b0141 0.22 -0.7 156,428 - yadN b0141 0.22 +1.8 156,428 - yadN b0141 0.22 +1.4 156,429 + yadN b0141 0.22 +1.8 156,429 + yadN b0141 0.22 +1.8 156,431 + yadN b0141 0.23 +1.8 156,431 + yadN b0141 0.23 +1.8 156,432 + yadN b0141 0.23 +1.8 156,432 + yadN b0141 0.23 +0.2 156,437 - yadN b0141 0.24 +0.8 156,437 - yadN b0141 0.24 +1.3 156,437 - yadN b0141 0.24 -1.5 156,437 - yadN b0141 0.24 +1.2 156,437 - yadN b0141 0.24 +0.4 156,437 - yadN b0141 0.24 -0.8 156,437 - yadN b0141 0.24 -1.6 156,439 - yadN b0141 0.24 -0.6 156,439 - yadN b0141 0.24 +0.0 156,439 - yadN b0141 0.24 +0.8 156,439 - yadN b0141 0.24 +0.4 156,439 - yadN b0141 0.24 -0.8 156,440 - yadN b0141 0.24 +1.8 156,486 + yadN b0141 0.32 +1.8 156,489 - yadN b0141 0.32 -0.0 156,489 - yadN b0141 0.32 +0.2 156,521 - yadN b0141 0.38 -0.3 156,533 - yadN b0141 0.40 -1.1 156,545 - yadN b0141 0.42 -0.7 156,547 - yadN b0141 0.42 -2.1 156,560 - yadN b0141 0.45 -2.6 156,600 + yadN b0141 0.51 +1.8 156,602 + yadN b0141 0.52 +1.8 156,605 - yadN b0141 0.52 -0.9 156,642 + yadN b0141 0.59 +0.2 156,661 + yadN b0141 0.62 +0.8 156,682 - yadN b0141 0.65 -0.4 156,682 - yadN b0141 0.65 +2.2 156,685 - yadN b0141 0.66 +0.8 156,685 - yadN b0141 0.66 -0.8 156,685 - yadN b0141 0.66 +1.1 156,687 - yadN b0141 0.66 -0.2 156,705 + yadN b0141 0.69 -1.2 156,711 + yadN b0141 0.70 +2.8 156,721 + yadN b0141 0.72 -1.2 156,721 + yadN b0141 0.72 -1.6 156,721 + yadN b0141 0.72 +2.0 156,722 + yadN b0141 0.72 -1.1 156,734 + yadN b0141 0.74 -0.2 156,735 + yadN b0141 0.75 -1.6 156,735 + yadN b0141 0.75 -0.4 156,744 + yadN b0141 0.76 +1.8 156,755 + yadN b0141 0.78 -1.7 156,757 + yadN b0141 0.78 -1.4 156,759 + yadN b0141 0.79 -0.6 156,786 + yadN b0141 0.83 +1.8 156,789 + yadN b0141 0.84 +4.6 156,793 + yadN b0141 0.84 -0.1 156,804 + yadN b0141 0.86 +0.3 156,823 + yadN b0141 0.90 +0.2 156,823 + yadN b0141 0.90 +1.3 156,825 + +0.6 156,827 + +0.2 156,827 + +0.4 156,833 - +1.8 156,834 + +0.3 156,849 + -0.4 156,849 + +0.5 156,849 + -1.8 156,850 + -0.2 156,854 + -0.4 156,854 + +0.4 157,218 - -1.2 157,279 - +0.3 157,291 - +0.3 157,301 - folK b0142 0.10 -1.3 157,745 + -1.1 157,870 - pcnB b0143 0.10 -0.1
Or see this region's nucleotide sequence