Strain Fitness in Escherichia coli BW25113 around b2952

Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyggR and yggS are separated by 17 nucleotidesyggS and yggT are separated by 17 nucleotidesyggT and yggU overlap by 4 nucleotidesyggU and yggV are separated by 7 nucleotidesyggV and yggW overlap by 8 nucleotides b2950: yggR - putative protein transport (VIMSS), at 3,092,122 to 3,093,102 yggR b2951: yggS - predicted enzyme (NCBI), at 3,093,120 to 3,093,824 yggS b2952: yggT - predicted inner membrane protein (NCBI), at 3,093,842 to 3,094,408 yggT b2953: yggU - orf, hypothetical protein (VIMSS), at 3,094,405 to 3,094,695 yggU b2954: yggV - putative deoxyribonucleotide triphosphate pyrophosphatase (NCBI), at 3,094,703 to 3,095,296 yggV b2955: yggW - coproporphyrinogen III oxidase (NCBI), at 3,095,289 to 3,096,425 yggW Position (kb) 3093 3094 3095Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3092.927 kb on + strand, within yggRat 3092.927 kb on + strandat 3092.930 kb on - strand, within yggRat 3092.953 kb on + strand, within yggRat 3093.045 kb on + strandat 3093.052 kb on - strandat 3093.052 kb on - strandat 3093.325 kb on + strand, within yggSat 3093.345 kb on + strand, within yggSat 3093.345 kb on + strand, within yggSat 3093.377 kb on + strand, within yggSat 3093.416 kb on + strand, within yggSat 3093.416 kb on + strand, within yggSat 3093.416 kb on + strand, within yggSat 3093.604 kb on + strand, within yggSat 3093.604 kb on + strand, within yggSat 3093.675 kb on + strand, within yggSat 3093.729 kb on - strand, within yggSat 3093.855 kb on - strandat 3093.855 kb on - strandat 3093.883 kb on + strandat 3093.891 kb on - strandat 3093.930 kb on - strand, within yggTat 3093.930 kb on - strand, within yggTat 3093.940 kb on - strand, within yggTat 3094.022 kb on + strand, within yggTat 3094.067 kb on - strand, within yggTat 3094.089 kb on - strand, within yggTat 3094.089 kb on - strand, within yggTat 3094.290 kb on + strand, within yggTat 3094.319 kb on + strand, within yggTat 3094.324 kb on + strand, within yggTat 3094.333 kb on + strand, within yggTat 3094.333 kb on + strand, within yggTat 3094.391 kb on - strandat 3094.400 kb on + strandat 3094.400 kb on + strandat 3094.451 kb on + strand, within yggUat 3094.451 kb on + strand, within yggUat 3094.542 kb on + strand, within yggUat 3094.775 kb on + strand, within yggVat 3094.879 kb on + strand, within yggVat 3094.920 kb on - strand, within yggVat 3094.948 kb on + strand, within yggVat 3095.006 kb on + strand, within yggVat 3095.095 kb on - strand, within yggVat 3095.115 kb on + strand, within yggVat 3095.136 kb on + strand, within yggVat 3095.299 kb on - strandat 3095.380 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50
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3,092,927 + yggR b2950 0.82 +0.2
3,092,927 + +1.4
3,092,930 - yggR b2950 0.82 +0.3
3,092,953 + yggR b2950 0.85 +0.1
3,093,045 + -0.0
3,093,052 - +0.9
3,093,052 - +0.1
3,093,325 + yggS b2951 0.29 -1.3
3,093,345 + yggS b2951 0.32 -0.1
3,093,345 + yggS b2951 0.32 +0.0
3,093,377 + yggS b2951 0.36 -0.2
3,093,416 + yggS b2951 0.42 -0.5
3,093,416 + yggS b2951 0.42 -0.7
3,093,416 + yggS b2951 0.42 -0.5
3,093,604 + yggS b2951 0.69 -0.9
3,093,604 + yggS b2951 0.69 -0.6
3,093,675 + yggS b2951 0.79 -0.7
3,093,729 - yggS b2951 0.86 -0.3
3,093,855 - -0.9
3,093,855 - -3.9
3,093,883 + -1.0
3,093,891 - -1.5
3,093,930 - yggT b2952 0.16 -1.3
3,093,930 - yggT b2952 0.16 -1.8
3,093,940 - yggT b2952 0.17 -3.4
3,094,022 + yggT b2952 0.32 -1.2
3,094,067 - yggT b2952 0.40 -0.9
3,094,089 - yggT b2952 0.44 -1.3
3,094,089 - yggT b2952 0.44 -0.5
3,094,290 + yggT b2952 0.79 -1.4
3,094,319 + yggT b2952 0.84 -0.9
3,094,324 + yggT b2952 0.85 -2.9
3,094,333 + yggT b2952 0.87 -0.8
3,094,333 + yggT b2952 0.87 -0.7
3,094,391 - -0.4
3,094,400 + -0.3
3,094,400 + -0.3
3,094,451 + yggU b2953 0.16 -0.2
3,094,451 + yggU b2953 0.16 +0.0
3,094,542 + yggU b2953 0.47 +0.3
3,094,775 + yggV b2954 0.12 -0.3
3,094,879 + yggV b2954 0.30 -1.6
3,094,920 - yggV b2954 0.37 -0.6
3,094,948 + yggV b2954 0.41 -0.7
3,095,006 + yggV b2954 0.51 -0.1
3,095,095 - yggV b2954 0.66 -1.4
3,095,115 + yggV b2954 0.69 +0.2
3,095,136 + yggV b2954 0.73 -0.2
3,095,299 - -0.2
3,095,380 + -1.1

Or see this region's nucleotide sequence