Strain Fitness in Escherichia coli BW25113 around b2513

Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfgL and yfgM are separated by 10 nucleotidesyfgM and hisS are separated by 17 nucleotides b2512: yfgL - protein assembly complex, lipoprotein component (NCBI), at 2,635,496 to 2,636,674 yfgL b2513: yfgM - hypothetical protein (NCBI), at 2,636,685 to 2,637,305 yfgM b2514: hisS - histidyl-tRNA synthetase (NCBI), at 2,637,323 to 2,638,597 hisS Position (kb) 2636 2637 2638Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2635.787 kb on - strand, within yfgLat 2635.787 kb on - strand, within yfgLat 2635.892 kb on - strand, within yfgLat 2635.892 kb on - strand, within yfgLat 2636.030 kb on - strand, within yfgLat 2636.078 kb on - strand, within yfgLat 2636.167 kb on - strand, within yfgLat 2636.230 kb on - strand, within yfgLat 2636.230 kb on - strand, within yfgLat 2636.264 kb on - strand, within yfgLat 2636.264 kb on - strand, within yfgLat 2636.268 kb on - strand, within yfgLat 2636.268 kb on - strand, within yfgLat 2636.312 kb on - strand, within yfgLat 2636.355 kb on - strand, within yfgLat 2636.428 kb on - strand, within yfgLat 2636.428 kb on - strand, within yfgLat 2636.433 kb on + strand, within yfgLat 2636.440 kb on - strand, within yfgLat 2636.822 kb on - strand, within yfgMat 2636.857 kb on + strand, within yfgMat 2636.878 kb on + strand, within yfgMat 2636.878 kb on + strand, within yfgMat 2636.897 kb on - strand, within yfgMat 2636.899 kb on + strand, within yfgMat 2636.905 kb on - strand, within yfgMat 2636.905 kb on - strand, within yfgMat 2636.907 kb on - strand, within yfgMat 2636.965 kb on - strand, within yfgMat 2637.054 kb on - strand, within yfgMat 2637.269 kb on + strandat 2637.269 kb on + strandat 2637.312 kb on - strandat 2637.328 kb on - strandat 2637.334 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50
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2,635,787 - yfgL b2512 0.25 -2.9
2,635,787 - yfgL b2512 0.25 -2.6
2,635,892 - yfgL b2512 0.34 -0.9
2,635,892 - yfgL b2512 0.34 -2.3
2,636,030 - yfgL b2512 0.45 -1.3
2,636,078 - yfgL b2512 0.49 -1.7
2,636,167 - yfgL b2512 0.57 -1.7
2,636,230 - yfgL b2512 0.62 -4.0
2,636,230 - yfgL b2512 0.62 -0.7
2,636,264 - yfgL b2512 0.65 -2.3
2,636,264 - yfgL b2512 0.65 -2.5
2,636,268 - yfgL b2512 0.65 -1.4
2,636,268 - yfgL b2512 0.65 -1.8
2,636,312 - yfgL b2512 0.69 -1.9
2,636,355 - yfgL b2512 0.73 -1.4
2,636,428 - yfgL b2512 0.79 -1.0
2,636,428 - yfgL b2512 0.79 -2.9
2,636,433 + yfgL b2512 0.79 -0.9
2,636,440 - yfgL b2512 0.80 -0.8
2,636,822 - yfgM b2513 0.22 -1.1
2,636,857 + yfgM b2513 0.28 -1.5
2,636,878 + yfgM b2513 0.31 -2.3
2,636,878 + yfgM b2513 0.31 -0.3
2,636,897 - yfgM b2513 0.34 -1.3
2,636,899 + yfgM b2513 0.34 -2.4
2,636,905 - yfgM b2513 0.35 -0.9
2,636,905 - yfgM b2513 0.35 -2.7
2,636,907 - yfgM b2513 0.36 -0.9
2,636,965 - yfgM b2513 0.45 -0.8
2,637,054 - yfgM b2513 0.59 -1.2
2,637,269 + -0.8
2,637,269 + -1.3
2,637,312 - -0.0
2,637,328 - +0.0
2,637,334 - +0.1

Or see this region's nucleotide sequence