Experiment: Outgrowth from conjugation with pRH22 in LB+Gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murP and b2430 are separated by 4 nucleotides b2430 and b2431 are separated by 239 nucleotides
b2429: murP - N-acetylmuramic acid phosphotransfer permease (NCBI), at 2,544,695 to 2,546,119
murP
b2430: b2430 - putative beta-lactamase (VIMSS), at 2,546,124 to 2,547,428
b2430
b2431: b2431 - orf, hypothetical protein (VIMSS), at 2,547,668 to 2,548,567
b2431
Position (kb)
2546
2547
2548 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2545.234 kb on - strand, within murP at 2545.234 kb on - strand, within murP at 2545.234 kb on - strand, within murP at 2545.236 kb on - strand, within murP at 2545.306 kb on - strand, within murP at 2545.347 kb on + strand, within murP at 2545.445 kb on - strand, within murP at 2545.471 kb on - strand, within murP at 2545.471 kb on - strand, within murP at 2545.494 kb on - strand, within murP at 2545.494 kb on - strand, within murP at 2545.559 kb on + strand, within murP at 2545.559 kb on + strand, within murP at 2545.620 kb on + strand, within murP at 2545.637 kb on + strand, within murP at 2545.761 kb on - strand, within murP at 2545.768 kb on + strand, within murP at 2545.768 kb on + strand, within murP at 2545.786 kb on - strand, within murP at 2545.886 kb on + strand, within murP at 2546.146 kb on - strand at 2546.233 kb on - strand at 2546.239 kb on + strand at 2546.412 kb on + strand, within b2430 at 2546.420 kb on - strand, within b2430 at 2546.420 kb on - strand, within b2430 at 2546.423 kb on + strand, within b2430 at 2546.489 kb on - strand, within b2430 at 2546.489 kb on - strand, within b2430 at 2546.663 kb on - strand, within b2430 at 2546.674 kb on + strand, within b2430 at 2546.758 kb on + strand, within b2430 at 2546.844 kb on + strand, within b2430 at 2546.969 kb on - strand, within b2430 at 2546.969 kb on - strand, within b2430 at 2547.030 kb on + strand, within b2430 at 2547.062 kb on - strand, within b2430 at 2547.062 kb on - strand, within b2430 at 2547.071 kb on - strand, within b2430 at 2547.087 kb on - strand, within b2430 at 2547.141 kb on - strand, within b2430 at 2547.319 kb on - strand at 2547.338 kb on - strand at 2547.495 kb on + strand at 2547.495 kb on + strand at 2547.774 kb on + strand, within b2431 at 2547.790 kb on + strand, within b2431 at 2547.798 kb on - strand, within b2431 at 2547.813 kb on + strand, within b2431 at 2547.824 kb on + strand, within b2431 at 2547.827 kb on - strand, within b2431 at 2547.830 kb on - strand, within b2431 at 2547.834 kb on + strand, within b2431 at 2547.929 kb on - strand at 2547.929 kb on - strand, within b2431 at 2547.937 kb on - strand, within b2431 at 2547.937 kb on - strand, within b2431 at 2547.991 kb on + strand, within b2431 at 2547.999 kb on - strand, within b2431 at 2548.032 kb on - strand, within b2431 at 2548.032 kb on - strand, within b2431 at 2548.044 kb on - strand, within b2431 at 2548.054 kb on + strand, within b2431 at 2548.054 kb on + strand, within b2431 at 2548.072 kb on + strand, within b2431 at 2548.163 kb on + strand, within b2431 at 2548.201 kb on + strand, within b2431 at 2548.203 kb on + strand, within b2431 at 2548.229 kb on - strand, within b2431 at 2548.322 kb on - strand, within b2431
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from conjugation with pRH22 in LB+Gent50 remove 2,545,234 - murP b2429 0.38 -0.6 2,545,234 - murP b2429 0.38 +0.2 2,545,234 - murP b2429 0.38 -0.2 2,545,236 - murP b2429 0.38 +0.5 2,545,306 - murP b2429 0.43 +0.1 2,545,347 + murP b2429 0.46 +0.1 2,545,445 - murP b2429 0.53 +0.0 2,545,471 - murP b2429 0.54 -0.7 2,545,471 - murP b2429 0.54 -0.6 2,545,494 - murP b2429 0.56 +1.3 2,545,494 - murP b2429 0.56 -0.3 2,545,559 + murP b2429 0.61 +0.2 2,545,559 + murP b2429 0.61 -0.0 2,545,620 + murP b2429 0.65 +0.8 2,545,637 + murP b2429 0.66 -0.1 2,545,761 - murP b2429 0.75 -0.2 2,545,768 + murP b2429 0.75 -0.4 2,545,768 + murP b2429 0.75 +0.1 2,545,786 - murP b2429 0.77 -0.7 2,545,886 + murP b2429 0.84 +0.1 2,546,146 - -0.9 2,546,233 - -0.1 2,546,239 + -0.1 2,546,412 + b2430 b2430 0.22 -0.5 2,546,420 - b2430 b2430 0.23 -0.3 2,546,420 - b2430 b2430 0.23 +0.5 2,546,423 + b2430 b2430 0.23 -0.4 2,546,489 - b2430 b2430 0.28 -0.9 2,546,489 - b2430 b2430 0.28 -0.0 2,546,663 - b2430 b2430 0.41 -0.2 2,546,674 + b2430 b2430 0.42 +0.3 2,546,758 + b2430 b2430 0.49 -0.1 2,546,844 + b2430 b2430 0.55 -0.4 2,546,969 - b2430 b2430 0.65 +0.4 2,546,969 - b2430 b2430 0.65 -0.0 2,547,030 + b2430 b2430 0.69 -0.3 2,547,062 - b2430 b2430 0.72 -0.8 2,547,062 - b2430 b2430 0.72 -0.8 2,547,071 - b2430 b2430 0.73 -0.4 2,547,087 - b2430 b2430 0.74 -0.8 2,547,141 - b2430 b2430 0.78 +0.0 2,547,319 - +3.6 2,547,338 - +0.3 2,547,495 + -0.2 2,547,495 + -0.2 2,547,774 + b2431 b2431 0.12 -1.2 2,547,790 + b2431 b2431 0.14 +0.1 2,547,798 - b2431 b2431 0.14 -0.5 2,547,813 + b2431 b2431 0.16 +0.3 2,547,824 + b2431 b2431 0.17 -0.5 2,547,827 - b2431 b2431 0.18 +1.1 2,547,830 - b2431 b2431 0.18 +0.6 2,547,834 + b2431 b2431 0.18 +0.0 2,547,929 - +0.8 2,547,929 - b2431 b2431 0.29 -0.6 2,547,937 - b2431 b2431 0.30 +0.3 2,547,937 - b2431 b2431 0.30 +0.2 2,547,991 + b2431 b2431 0.36 +1.5 2,547,999 - b2431 b2431 0.37 +0.6 2,548,032 - b2431 b2431 0.40 -0.4 2,548,032 - b2431 b2431 0.40 +0.1 2,548,044 - b2431 b2431 0.42 +0.8 2,548,054 + b2431 b2431 0.43 -0.8 2,548,054 + b2431 b2431 0.43 +0.3 2,548,072 + b2431 b2431 0.45 -0.6 2,548,163 + b2431 b2431 0.55 +0.1 2,548,201 + b2431 b2431 0.59 -0.0 2,548,203 + b2431 b2431 0.59 -0.4 2,548,229 - b2431 b2431 0.62 +0.4 2,548,322 - b2431 b2431 0.73 -0.4
Or see this region's nucleotide sequence