Strain Fitness in Escherichia coli BW25113 around b2482

Experiment: mzaABCDE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbcp and hyfA are separated by 252 nucleotideshyfA and hyfB overlap by 1 nucleotideshyfB and hyfC are separated by 10 nucleotideshyfC and hyfD are separated by 16 nucleotides b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970 bcp b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840 hyfA b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858 hyfB b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816 hyfC b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272 hyfD Position (kb) 2599 2600 2601 2602Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2598.938 kb on + strandat 2598.967 kb on - strandat 2598.976 kb on - strandat 2599.005 kb on + strandat 2599.230 kb on + strandat 2599.238 kb on - strandat 2599.319 kb on - strand, within hyfAat 2599.319 kb on - strand, within hyfAat 2599.362 kb on + strand, within hyfAat 2599.370 kb on - strand, within hyfAat 2599.426 kb on + strand, within hyfAat 2599.488 kb on - strand, within hyfAat 2599.528 kb on - strand, within hyfAat 2599.913 kb on - strandat 2599.998 kb on + strandat 2600.132 kb on + strand, within hyfBat 2600.135 kb on - strand, within hyfBat 2600.340 kb on - strand, within hyfBat 2600.517 kb on - strand, within hyfBat 2600.527 kb on + strand, within hyfBat 2600.577 kb on - strand, within hyfBat 2600.621 kb on - strand, within hyfBat 2600.621 kb on - strand, within hyfBat 2600.759 kb on - strand, within hyfBat 2600.889 kb on + strand, within hyfBat 2601.194 kb on + strand, within hyfBat 2601.202 kb on - strand, within hyfBat 2601.245 kb on - strand, within hyfBat 2601.245 kb on - strand, within hyfBat 2601.404 kb on - strand, within hyfBat 2601.421 kb on + strand, within hyfBat 2601.434 kb on + strand, within hyfBat 2601.588 kb on + strand, within hyfBat 2601.644 kb on + strand, within hyfBat 2601.644 kb on + strand, within hyfBat 2601.652 kb on - strand, within hyfBat 2601.652 kb on - strand, within hyfBat 2601.701 kb on - strandat 2601.701 kb on - strandat 2601.810 kb on + strandat 2601.925 kb on + strandat 2601.925 kb on + strandat 2601.988 kb on - strand, within hyfCat 2602.127 kb on + strand, within hyfCat 2602.127 kb on + strand, within hyfCat 2602.150 kb on - strand, within hyfCat 2602.199 kb on - strand, within hyfCat 2602.199 kb on - strand, within hyfCat 2602.325 kb on + strand, within hyfCat 2602.350 kb on + strand, within hyfCat 2602.379 kb on + strand, within hyfCat 2602.382 kb on - strand, within hyfCat 2602.432 kb on - strand, within hyfCat 2602.620 kb on - strand, within hyfCat 2602.620 kb on - strand, within hyfCat 2602.813 kb on + strandat 2602.813 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction mzaABCDE
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2,598,938 + +0.3
2,598,967 - -1.8
2,598,976 - -0.7
2,599,005 + +0.9
2,599,230 + +0.5
2,599,238 - -1.6
2,599,319 - hyfA b2481 0.16 +1.3
2,599,319 - hyfA b2481 0.16 +0.0
2,599,362 + hyfA b2481 0.22 -0.2
2,599,370 - hyfA b2481 0.24 +0.1
2,599,426 + hyfA b2481 0.33 -2.8
2,599,488 - hyfA b2481 0.43 -0.5
2,599,528 - hyfA b2481 0.49 -0.4
2,599,913 - -0.1
2,599,998 + -1.9
2,600,132 + hyfB b2482 0.14 +0.1
2,600,135 - hyfB b2482 0.15 +0.1
2,600,340 - hyfB b2482 0.25 +0.3
2,600,517 - hyfB b2482 0.34 -0.1
2,600,527 + hyfB b2482 0.34 -0.4
2,600,577 - hyfB b2482 0.37 +0.3
2,600,621 - hyfB b2482 0.39 -0.4
2,600,621 - hyfB b2482 0.39 +0.7
2,600,759 - hyfB b2482 0.46 -0.4
2,600,889 + hyfB b2482 0.52 -1.1
2,601,194 + hyfB b2482 0.67 -1.1
2,601,202 - hyfB b2482 0.67 -0.5
2,601,245 - hyfB b2482 0.70 +0.0
2,601,245 - hyfB b2482 0.70 +0.6
2,601,404 - hyfB b2482 0.77 -0.9
2,601,421 + hyfB b2482 0.78 +0.1
2,601,434 + hyfB b2482 0.79 +0.4
2,601,588 + hyfB b2482 0.87 -2.0
2,601,644 + hyfB b2482 0.89 -2.6
2,601,644 + hyfB b2482 0.89 -0.1
2,601,652 - hyfB b2482 0.90 -3.8
2,601,652 - hyfB b2482 0.90 -0.8
2,601,701 - +0.5
2,601,701 - +0.1
2,601,810 + +0.5
2,601,925 + -0.4
2,601,925 + +0.5
2,601,988 - hyfC b2483 0.13 +0.2
2,602,127 + hyfC b2483 0.27 +1.0
2,602,127 + hyfC b2483 0.27 +1.7
2,602,150 - hyfC b2483 0.30 -1.1
2,602,199 - hyfC b2483 0.35 -0.9
2,602,199 - hyfC b2483 0.35 +1.1
2,602,325 + hyfC b2483 0.48 +0.4
2,602,350 + hyfC b2483 0.51 +0.2
2,602,379 + hyfC b2483 0.54 -1.2
2,602,382 - hyfC b2483 0.54 -0.8
2,602,432 - hyfC b2483 0.59 +0.1
2,602,620 - hyfC b2483 0.79 +0.6
2,602,620 - hyfC b2483 0.79 +1.8
2,602,813 + +0.2
2,602,813 + +1.1

Or see this region's nucleotide sequence