Experiment: mzaABCDE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebC and nudB are separated by 28 nucleotides nudB and aspS are separated by 117 nucleotides aspS and yecD are separated by 309 nucleotides yecD and yecE overlap by 4 nucleotides
b1864: yebC - hypothetical protein (NCBI), at 1,945,435 to 1,946,175
yebC
b1865: nudB - dATP pyrophosphohydrolase (NCBI), at 1,946,204 to 1,946,656
nudB
b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546
aspS
b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422
yecD
b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237
yecE
Position (kb)
1946
1947
1948
1949 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1945.975 kb on + strand, within yebC at 1945.975 kb on + strand, within yebC at 1945.997 kb on + strand, within yebC at 1946.123 kb on - strand at 1946.247 kb on + strand at 1946.247 kb on + strand at 1946.435 kb on + strand, within nudB at 1946.471 kb on - strand, within nudB at 1946.471 kb on - strand, within nudB at 1946.636 kb on + strand at 1946.644 kb on - strand at 1948.556 kb on - strand at 1948.718 kb on - strand at 1948.761 kb on + strand at 1948.771 kb on + strand at 1948.859 kb on - strand at 1948.859 kb on - strand at 1948.932 kb on + strand, within yecD at 1949.048 kb on + strand, within yecD at 1949.173 kb on + strand, within yecD at 1949.173 kb on + strand, within yecD at 1949.179 kb on - strand, within yecD at 1949.186 kb on + strand, within yecD at 1949.186 kb on + strand, within yecD at 1949.189 kb on - strand, within yecD at 1949.228 kb on + strand, within yecD at 1949.228 kb on + strand, within yecD at 1949.228 kb on - strand, within yecD at 1949.228 kb on - strand, within yecD at 1949.231 kb on - strand, within yecD at 1949.349 kb on + strand, within yecD at 1949.404 kb on - strand at 1949.404 kb on - strand at 1949.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mzaABCDE remove 1,945,975 + yebC b1864 0.73 -2.6 1,945,975 + yebC b1864 0.73 -1.3 1,945,997 + yebC b1864 0.76 -1.2 1,946,123 - +1.2 1,946,247 + -3.3 1,946,247 + -3.6 1,946,435 + nudB b1865 0.51 -2.2 1,946,471 - nudB b1865 0.59 -4.2 1,946,471 - nudB b1865 0.59 -3.8 1,946,636 + -1.7 1,946,644 - +0.3 1,948,556 - -0.1 1,948,718 - -0.9 1,948,761 + -0.6 1,948,771 + +0.0 1,948,859 - +0.7 1,948,859 - +0.6 1,948,932 + yecD b1867 0.13 +0.7 1,949,048 + yecD b1867 0.34 -2.6 1,949,173 + yecD b1867 0.56 +0.0 1,949,173 + yecD b1867 0.56 -1.1 1,949,179 - yecD b1867 0.57 -4.7 1,949,186 + yecD b1867 0.58 -0.5 1,949,186 + yecD b1867 0.58 -0.0 1,949,189 - yecD b1867 0.59 +0.2 1,949,228 + yecD b1867 0.66 +1.0 1,949,228 + yecD b1867 0.66 +0.8 1,949,228 - yecD b1867 0.66 -0.8 1,949,228 - yecD b1867 0.66 -1.5 1,949,231 - yecD b1867 0.66 +1.2 1,949,349 + yecD b1867 0.87 -0.4 1,949,404 - -0.7 1,949,404 - -0.4 1,949,442 - +0.5
Or see this region's nucleotide sequence