Experiment: mzaABCDE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murB and birA overlap by 4 nucleotides birA and coaA are separated by 28 nucleotides coaA and thrU are separated by 361 nucleotides thrU and tyrU are separated by 8 nucleotides tyrU and glyT are separated by 116 nucleotides glyT and thrT are separated by 6 nucleotides thrT and tufB are separated by 114 nucleotides
b3972: murB - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI), at 4,170,080 to 4,171,108
murB
b3973: birA - biotin--protein ligase (NCBI), at 4,171,105 to 4,172,070
birA
b3974: coaA - pantothenate kinase (NCBI), at 4,172,099 to 4,173,049
coaA
b3976: thrU - tRNA-Thr (NCBI), at 4,173,411 to 4,173,486
thrU
b3977: tyrU - tRNA-Tyr (NCBI), at 4,173,495 to 4,173,579
tyrU
b3978: glyT - tRNA-Gly (NCBI), at 4,173,696 to 4,173,770
glyT
b3979: thrT - tRNA-Thr (NCBI), at 4,173,777 to 4,173,852
thrT
b3980: tufB - protein chain elongation factor EF-Tu (duplicate of tufA) (NCBI), at 4,173,967 to 4,175,151
tufB
Position (kb)
4172
4173
4174 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4171.110 kb on + strand at 4171.236 kb on + strand, within birA at 4171.284 kb on + strand, within birA at 4172.112 kb on - strand at 4172.981 kb on - strand at 4172.981 kb on - strand at 4173.047 kb on - strand at 4173.074 kb on - strand at 4173.125 kb on + strand at 4173.139 kb on - strand at 4173.139 kb on - strand at 4173.147 kb on - strand at 4173.155 kb on - strand at 4173.219 kb on - strand at 4173.308 kb on - strand at 4173.399 kb on + strand at 4173.546 kb on + strand, within tyrU at 4173.781 kb on + strand at 4173.813 kb on - strand, within thrT at 4173.861 kb on - strand at 4173.861 kb on - strand at 4173.912 kb on - strand at 4173.916 kb on + strand at 4173.929 kb on + strand at 4173.989 kb on + strand at 4174.016 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mzaABCDE remove 4,171,110 + +1.1 4,171,236 + birA b3973 0.14 +0.5 4,171,284 + birA b3973 0.19 +0.2 4,172,112 - -2.6 4,172,981 - +1.2 4,172,981 - -3.6 4,173,047 - -0.1 4,173,074 - -0.2 4,173,125 + -0.6 4,173,139 - -0.5 4,173,139 - -0.8 4,173,147 - +0.3 4,173,155 - -1.0 4,173,219 - -2.1 4,173,308 - -1.3 4,173,399 + +1.2 4,173,546 + tyrU b3977 0.60 -3.8 4,173,781 + +2.2 4,173,813 - thrT b3979 0.47 +0.2 4,173,861 - -0.4 4,173,861 - -0.2 4,173,912 - -0.2 4,173,916 + -0.7 4,173,929 + -0.6 4,173,989 + -1.2 4,174,016 - -1.2
Or see this region's nucleotide sequence