Strain Fitness in Escherichia coli BW25113 around b3974

Experiment: mzaABCDE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurB and birA overlap by 4 nucleotidesbirA and coaA are separated by 28 nucleotidescoaA and thrU are separated by 361 nucleotidesthrU and tyrU are separated by 8 nucleotidestyrU and glyT are separated by 116 nucleotidesglyT and thrT are separated by 6 nucleotidesthrT and tufB are separated by 114 nucleotides b3972: murB - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI), at 4,170,080 to 4,171,108 murB b3973: birA - biotin--protein ligase (NCBI), at 4,171,105 to 4,172,070 birA b3974: coaA - pantothenate kinase (NCBI), at 4,172,099 to 4,173,049 coaA b3976: thrU - tRNA-Thr (NCBI), at 4,173,411 to 4,173,486 thrU b3977: tyrU - tRNA-Tyr (NCBI), at 4,173,495 to 4,173,579 tyrU b3978: glyT - tRNA-Gly (NCBI), at 4,173,696 to 4,173,770 glyT b3979: thrT - tRNA-Thr (NCBI), at 4,173,777 to 4,173,852 thrT b3980: tufB - protein chain elongation factor EF-Tu (duplicate of tufA) (NCBI), at 4,173,967 to 4,175,151 tufB Position (kb) 4172 4173 4174Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4171.110 kb on + strandat 4171.236 kb on + strand, within birAat 4171.284 kb on + strand, within birAat 4172.112 kb on - strandat 4172.981 kb on - strandat 4172.981 kb on - strandat 4173.047 kb on - strandat 4173.074 kb on - strandat 4173.125 kb on + strandat 4173.139 kb on - strandat 4173.139 kb on - strandat 4173.147 kb on - strandat 4173.155 kb on - strandat 4173.219 kb on - strandat 4173.308 kb on - strandat 4173.399 kb on + strandat 4173.546 kb on + strand, within tyrUat 4173.781 kb on + strandat 4173.813 kb on - strand, within thrTat 4173.861 kb on - strandat 4173.861 kb on - strandat 4173.912 kb on - strandat 4173.916 kb on + strandat 4173.929 kb on + strandat 4173.989 kb on + strandat 4174.016 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction mzaABCDE
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4,171,110 + +1.1
4,171,236 + birA b3973 0.14 +0.5
4,171,284 + birA b3973 0.19 +0.2
4,172,112 - -2.6
4,172,981 - +1.2
4,172,981 - -3.6
4,173,047 - -0.1
4,173,074 - -0.2
4,173,125 + -0.6
4,173,139 - -0.5
4,173,139 - -0.8
4,173,147 - +0.3
4,173,155 - -1.0
4,173,219 - -2.1
4,173,308 - -1.3
4,173,399 + +1.2
4,173,546 + tyrU b3977 0.60 -3.8
4,173,781 + +2.2
4,173,813 - thrT b3979 0.47 +0.2
4,173,861 - -0.4
4,173,861 - -0.2
4,173,912 - -0.2
4,173,916 + -0.7
4,173,929 + -0.6
4,173,989 + -1.2
4,174,016 - -1.2

Or see this region's nucleotide sequence