Experiment: mzaABCDE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluD and yfiO are separated by 134 nucleotides yfiO and yfiA are separated by 270 nucleotides yfiA and pheL are separated by 103 nucleotides pheL and pheA are separated by 98 nucleotides
b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033
rluD
b2595: yfiO - predicted lipoprotein (NCBI), at 2,734,168 to 2,734,905
yfiO
b2597: yfiA - cold shock protein associated with 30S ribosomal subunit (NCBI), at 2,735,176 to 2,735,517
yfiA
b2598: pheL - pheA gene leader peptide (NCBI), at 2,735,621 to 2,735,668
pheL
b2599: pheA - fused chorismate mutase P/prephenate dehydratase (NCBI), at 2,735,767 to 2,736,927
pheA
Position (kb)
2734
2735 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2734.040 kb on - strand at 2734.040 kb on - strand at 2734.042 kb on - strand at 2734.078 kb on + strand at 2734.095 kb on + strand at 2734.095 kb on + strand at 2734.103 kb on - strand at 2734.165 kb on + strand at 2734.833 kb on + strand at 2734.848 kb on - strand at 2734.979 kb on + strand at 2734.996 kb on - strand at 2734.996 kb on - strand at 2735.339 kb on + strand, within yfiA at 2735.512 kb on + strand at 2735.520 kb on - strand at 2735.555 kb on - strand at 2735.555 kb on - strand at 2735.688 kb on + strand at 2735.719 kb on - strand at 2735.782 kb on + strand at 2735.782 kb on + strand at 2735.877 kb on + strand at 2735.877 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mzaABCDE remove 2,734,040 - -1.0 2,734,040 - +1.6 2,734,042 - +0.5 2,734,078 + -2.8 2,734,095 + +0.2 2,734,095 + -0.2 2,734,103 - -4.8 2,734,165 + +0.7 2,734,833 + -1.7 2,734,848 - -2.2 2,734,979 + -0.4 2,734,996 - +1.0 2,734,996 - +0.4 2,735,339 + yfiA b2597 0.48 +1.8 2,735,512 + +0.3 2,735,520 - -1.0 2,735,555 - +1.1 2,735,555 - +1.9 2,735,688 + -0.4 2,735,719 - +1.6 2,735,782 + -2.7 2,735,782 + +0.3 2,735,877 + +1.3 2,735,877 + -0.5
Or see this region's nucleotide sequence