Strain Fitness in Escherichia coli BW25113 around b2595

Experiment: mzaABCDE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrluD and yfiO are separated by 134 nucleotidesyfiO and yfiA are separated by 270 nucleotidesyfiA and pheL are separated by 103 nucleotidespheL and pheA are separated by 98 nucleotides b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033 rluD b2595: yfiO - predicted lipoprotein (NCBI), at 2,734,168 to 2,734,905 yfiO b2597: yfiA - cold shock protein associated with 30S ribosomal subunit (NCBI), at 2,735,176 to 2,735,517 yfiA b2598: pheL - pheA gene leader peptide (NCBI), at 2,735,621 to 2,735,668 pheL b2599: pheA - fused chorismate mutase P/prephenate dehydratase (NCBI), at 2,735,767 to 2,736,927 pheA Position (kb) 2734 2735Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2734.040 kb on - strandat 2734.040 kb on - strandat 2734.042 kb on - strandat 2734.078 kb on + strandat 2734.095 kb on + strandat 2734.095 kb on + strandat 2734.103 kb on - strandat 2734.165 kb on + strandat 2734.833 kb on + strandat 2734.848 kb on - strandat 2734.979 kb on + strandat 2734.996 kb on - strandat 2734.996 kb on - strandat 2735.339 kb on + strand, within yfiAat 2735.512 kb on + strandat 2735.520 kb on - strandat 2735.555 kb on - strandat 2735.555 kb on - strandat 2735.688 kb on + strandat 2735.719 kb on - strandat 2735.782 kb on + strandat 2735.782 kb on + strandat 2735.877 kb on + strandat 2735.877 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction mzaABCDE
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2,734,040 - -1.0
2,734,040 - +1.6
2,734,042 - +0.5
2,734,078 + -2.8
2,734,095 + +0.2
2,734,095 + -0.2
2,734,103 - -4.8
2,734,165 + +0.7
2,734,833 + -1.7
2,734,848 - -2.2
2,734,979 + -0.4
2,734,996 - +1.0
2,734,996 - +0.4
2,735,339 + yfiA b2597 0.48 +1.8
2,735,512 + +0.3
2,735,520 - -1.0
2,735,555 - +1.1
2,735,555 - +1.9
2,735,688 + -0.4
2,735,719 - +1.6
2,735,782 + -2.7
2,735,782 + +0.3
2,735,877 + +1.3
2,735,877 + -0.5

Or see this region's nucleotide sequence