Strain Fitness in Escherichia coli BW25113 around b3783

Experiment: ppI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrhlB and trxA are separated by 130 nucleotidestrxA and rho are separated by 326 nucleotidesrho and rfe are separated by 239 nucleotides b3780: rhlB - ATP-dependent RNA helicase (NCBI), at 3,962,388 to 3,963,653 rhlB b3781: trxA - thioredoxin 1 (VIMSS), at 3,963,784 to 3,964,113 trxA b3783: rho - transcription termination factor Rho (NCBI), at 3,964,440 to 3,965,699 rho b3784: rfe - UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI), at 3,965,939 to 3,967,042 rfe Position (kb) 3964 3965 3966Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 3963.445 kb on - strand, within rhlBat 3963.450 kb on - strand, within rhlBat 3963.503 kb on - strand, within rhlBat 3963.513 kb on - strand, within rhlBat 3963.542 kb on + strandat 3963.542 kb on + strandat 3963.550 kb on - strandat 3963.553 kb on + strandat 3963.553 kb on + strandat 3963.561 kb on - strandat 3963.561 kb on - strandat 3963.605 kb on - strandat 3963.632 kb on - strandat 3963.655 kb on + strandat 3963.655 kb on + strandat 3963.694 kb on - strandat 3963.758 kb on - strandat 3963.764 kb on - strandat 3963.769 kb on - strandat 3963.780 kb on + strandat 3963.788 kb on - strandat 3963.788 kb on - strandat 3963.829 kb on + strandat 3963.837 kb on - strand, within trxAat 3963.839 kb on + strand, within trxAat 3963.856 kb on - strand, within trxAat 3963.957 kb on + strand, within trxAat 3963.975 kb on + strand, within trxAat 3963.975 kb on + strand, within trxAat 3963.975 kb on + strand, within trxAat 3964.021 kb on - strand, within trxAat 3964.026 kb on - strand, within trxAat 3964.068 kb on + strand, within trxAat 3964.068 kb on + strandat 3964.080 kb on + strand, within trxAat 3964.110 kb on - strandat 3964.110 kb on - strandat 3964.155 kb on + strandat 3964.179 kb on + strandat 3964.254 kb on + strandat 3964.308 kb on + strandat 3964.318 kb on - strandat 3964.320 kb on + strandat 3964.320 kb on + strandat 3964.320 kb on + strandat 3964.355 kb on + strandat 3964.378 kb on + strandat 3964.386 kb on - strandat 3964.460 kb on + strandat 3964.481 kb on + strandat 3965.849 kb on + strandat 3965.942 kb on + strandat 3965.956 kb on + strandat 3965.956 kb on + strandat 3965.958 kb on + strandat 3965.958 kb on + strandat 3965.958 kb on + strandat 3965.966 kb on - strandat 3965.966 kb on - strandat 3965.983 kb on + strandat 3965.996 kb on - strandat 3966.035 kb on + strandat 3966.035 kb on + strandat 3966.070 kb on + strand, within rfeat 3966.080 kb on + strand, within rfeat 3966.105 kb on + strand, within rfeat 3966.139 kb on + strand, within rfeat 3966.141 kb on + strand, within rfeat 3966.144 kb on + strand, within rfeat 3966.144 kb on + strand, within rfeat 3966.149 kb on - strand, within rfeat 3966.160 kb on + strand, within rfeat 3966.168 kb on - strand, within rfeat 3966.223 kb on - strand, within rfeat 3966.223 kb on - strand, within rfeat 3966.240 kb on + strand, within rfeat 3966.240 kb on + strand, within rfeat 3966.240 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.242 kb on + strand, within rfeat 3966.248 kb on - strand, within rfeat 3966.248 kb on - strand, within rfeat 3966.248 kb on - strand, within rfeat 3966.257 kb on + strand, within rfeat 3966.257 kb on + strand, within rfeat 3966.266 kb on + strand, within rfeat 3966.266 kb on + strand, within rfeat 3966.274 kb on + strand, within rfeat 3966.362 kb on + strand, within rfeat 3966.370 kb on - strand, within rfeat 3966.370 kb on - strand, within rfeat 3966.402 kb on - strand, within rfeat 3966.428 kb on + strand, within rfeat 3966.441 kb on - strand, within rfeat 3966.441 kb on - strand, within rfeat 3966.441 kb on - strand, within rfeat 3966.441 kb on - strand, within rfeat 3966.452 kb on - strand, within rfeat 3966.452 kb on - strandat 3966.484 kb on + strand, within rfeat 3966.505 kb on - strand, within rfeat 3966.529 kb on + strand, within rfeat 3966.537 kb on - strand, within rfeat 3966.540 kb on + strand, within rfeat 3966.542 kb on - strand, within rfeat 3966.554 kb on - strand, within rfeat 3966.555 kb on + strand, within rfeat 3966.555 kb on + strand, within rfeat 3966.573 kb on - strand, within rfeat 3966.599 kb on + strand, within rfeat 3966.599 kb on + strand, within rfeat 3966.599 kb on + strand, within rfeat 3966.636 kb on + strand, within rfeat 3966.646 kb on + strand, within rfeat 3966.646 kb on + strand, within rfeat 3966.682 kb on + strand, within rfe

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Per-strain Table

Position Strand Gene LocusTag Fraction ppI
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3,963,445 - rhlB b3780 0.83 +0.7
3,963,450 - rhlB b3780 0.84 -1.1
3,963,503 - rhlB b3780 0.88 -0.9
3,963,513 - rhlB b3780 0.89 -1.5
3,963,542 + -3.4
3,963,542 + +0.1
3,963,550 - -0.4
3,963,553 + -0.8
3,963,553 + -1.9
3,963,561 - -0.3
3,963,561 - -1.3
3,963,605 - -1.0
3,963,632 - +0.3
3,963,655 + -0.5
3,963,655 + -0.5
3,963,694 - -0.4
3,963,758 - +0.4
3,963,764 - -1.0
3,963,769 - +1.3
3,963,780 + +0.9
3,963,788 - +0.7
3,963,788 - +0.4
3,963,829 + +0.3
3,963,837 - trxA b3781 0.16 +0.3
3,963,839 + trxA b3781 0.17 -2.9
3,963,856 - trxA b3781 0.22 -3.7
3,963,957 + trxA b3781 0.52 -4.1
3,963,975 + trxA b3781 0.58 +0.3
3,963,975 + trxA b3781 0.58 -0.7
3,963,975 + trxA b3781 0.58 -1.4
3,964,021 - trxA b3781 0.72 -0.5
3,964,026 - trxA b3781 0.73 -2.1
3,964,068 + trxA b3781 0.86 -2.3
3,964,068 + +0.5
3,964,080 + trxA b3781 0.90 -1.0
3,964,110 - -2.6
3,964,110 - -1.1
3,964,155 + +1.4
3,964,179 + -0.7
3,964,254 + -0.3
3,964,308 + +1.2
3,964,318 - -0.7
3,964,320 + +0.0
3,964,320 + +0.1
3,964,320 + +1.2
3,964,355 + +0.5
3,964,378 + -0.6
3,964,386 - -2.5
3,964,460 + -2.0
3,964,481 + +1.9
3,965,849 + -0.6
3,965,942 + -0.1
3,965,956 + -2.4
3,965,956 + -1.7
3,965,958 + -4.2
3,965,958 + -3.9
3,965,958 + -3.0
3,965,966 - -2.7
3,965,966 - -4.8
3,965,983 + -3.7
3,965,996 - -4.3
3,966,035 + -3.5
3,966,035 + -3.5
3,966,070 + rfe b3784 0.12 -6.1
3,966,080 + rfe b3784 0.13 -3.1
3,966,105 + rfe b3784 0.15 -0.9
3,966,139 + rfe b3784 0.18 -0.8
3,966,141 + rfe b3784 0.18 -3.5
3,966,144 + rfe b3784 0.19 -1.7
3,966,144 + rfe b3784 0.19 -2.7
3,966,149 - rfe b3784 0.19 -4.2
3,966,160 + rfe b3784 0.20 -4.1
3,966,168 - rfe b3784 0.21 -4.6
3,966,223 - rfe b3784 0.26 -3.4
3,966,223 - rfe b3784 0.26 -3.6
3,966,240 + rfe b3784 0.27 -3.8
3,966,240 + rfe b3784 0.27 -2.2
3,966,240 + rfe b3784 0.27 -2.9
3,966,242 + rfe b3784 0.27 -3.7
3,966,242 + rfe b3784 0.27 -2.7
3,966,242 + rfe b3784 0.27 -3.9
3,966,242 + rfe b3784 0.27 -2.6
3,966,242 + rfe b3784 0.27 -2.1
3,966,242 + rfe b3784 0.27 -3.0
3,966,242 + rfe b3784 0.27 -1.5
3,966,248 - rfe b3784 0.28 -3.8
3,966,248 - rfe b3784 0.28 -2.0
3,966,248 - rfe b3784 0.28 -3.4
3,966,257 + rfe b3784 0.29 -3.5
3,966,257 + rfe b3784 0.29 -3.8
3,966,266 + rfe b3784 0.30 -2.3
3,966,266 + rfe b3784 0.30 -4.0
3,966,274 + rfe b3784 0.30 -3.0
3,966,362 + rfe b3784 0.38 -2.2
3,966,370 - rfe b3784 0.39 -3.1
3,966,370 - rfe b3784 0.39 -3.3
3,966,402 - rfe b3784 0.42 -4.0
3,966,428 + rfe b3784 0.44 -0.9
3,966,441 - rfe b3784 0.45 -4.5
3,966,441 - rfe b3784 0.45 -2.7
3,966,441 - rfe b3784 0.45 -4.3
3,966,441 - rfe b3784 0.45 -4.9
3,966,452 - rfe b3784 0.46 -0.9
3,966,452 - -2.7
3,966,484 + rfe b3784 0.49 -2.1
3,966,505 - rfe b3784 0.51 -4.4
3,966,529 + rfe b3784 0.53 -3.3
3,966,537 - rfe b3784 0.54 -4.7
3,966,540 + rfe b3784 0.54 -5.0
3,966,542 - rfe b3784 0.55 -1.7
3,966,554 - rfe b3784 0.56 -4.9
3,966,555 + rfe b3784 0.56 -4.1
3,966,555 + rfe b3784 0.56 -2.6
3,966,573 - rfe b3784 0.57 -4.6
3,966,599 + rfe b3784 0.60 -3.2
3,966,599 + rfe b3784 0.60 -4.2
3,966,599 + rfe b3784 0.60 -3.2
3,966,636 + rfe b3784 0.63 -4.8
3,966,646 + rfe b3784 0.64 -5.1
3,966,646 + rfe b3784 0.64 -5.3
3,966,682 + rfe b3784 0.67 -3.2

Or see this region's nucleotide sequence