Experiment: ppI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciX_1 and yciX are separated by 2 nucleotides yciX and acnA are separated by 372 nucleotides acnA and ribA are separated by 63 nucleotides ribA and pgpB are separated by 169 nucleotides
b4522: yciX_1 - hypothetical protein (NCBI), at 1,333,184 to 1,333,312
_1
b4523: yciX - hypothetical protein (RefSeq), at 1,333,315 to 1,333,482
yciX
b1276: acnA - aconitate hydratase (NCBI), at 1,333,855 to 1,336,530
acnA
b1277: ribA - GTP cyclohydrolase II protein (NCBI), at 1,336,594 to 1,337,184
ribA
b1278: pgpB - phosphatidylglycerophosphatase B (NCBI), at 1,337,354 to 1,338,118
pgpB
Position (kb)
1333
1334
1335
1336
1337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1332.922 kb on + strand at 1332.930 kb on - strand at 1332.930 kb on - strand at 1333.156 kb on + strand at 1333.158 kb on - strand at 1333.160 kb on - strand at 1333.197 kb on + strand, within yciX_1 at 1333.198 kb on + strand, within yciX_1 at 1333.275 kb on - strand, within yciX_1 at 1333.286 kb on - strand, within yciX_1 at 1333.286 kb on - strand, within yciX_1 at 1333.301 kb on + strand at 1333.301 kb on + strand at 1333.311 kb on + strand at 1333.320 kb on - strand at 1333.320 kb on - strand at 1333.322 kb on + strand at 1333.322 kb on + strand at 1333.345 kb on + strand, within yciX at 1333.345 kb on + strand, within yciX at 1333.345 kb on + strand, within yciX at 1333.349 kb on + strand, within yciX at 1333.418 kb on + strand, within yciX at 1333.420 kb on - strand, within yciX at 1333.434 kb on - strand, within yciX at 1333.450 kb on - strand, within yciX at 1333.450 kb on - strand, within yciX at 1333.525 kb on + strand at 1333.538 kb on + strand at 1333.578 kb on + strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.630 kb on - strand at 1333.655 kb on + strand at 1333.670 kb on + strand at 1333.670 kb on + strand at 1333.678 kb on - strand at 1333.794 kb on + strand at 1333.794 kb on + strand at 1333.818 kb on + strand at 1333.838 kb on + strand at 1333.841 kb on - strand at 1333.847 kb on - strand at 1333.873 kb on - strand at 1333.932 kb on - strand at 1334.080 kb on - strand at 1334.080 kb on - strand at 1334.663 kb on - strand, within acnA at 1334.824 kb on - strand, within acnA at 1334.824 kb on - strand, within acnA at 1334.962 kb on + strand, within acnA at 1335.010 kb on + strand, within acnA at 1335.010 kb on + strand, within acnA at 1335.167 kb on + strand, within acnA at 1335.181 kb on + strand, within acnA at 1335.573 kb on - strand, within acnA at 1335.601 kb on - strand, within acnA at 1335.601 kb on - strand, within acnA at 1335.669 kb on + strand, within acnA at 1335.669 kb on + strand, within acnA at 1335.825 kb on - strand, within acnA at 1335.825 kb on - strand, within acnA at 1335.902 kb on - strand, within acnA at 1335.902 kb on - strand, within acnA at 1336.129 kb on - strand, within acnA at 1336.223 kb on + strand, within acnA at 1337.219 kb on - strand at 1337.219 kb on - strand at 1337.498 kb on + strand, within pgpB at 1337.503 kb on + strand, within pgpB at 1337.522 kb on - strand, within pgpB
Per-strain Table
Position Strand Gene LocusTag Fraction ppI remove 1,332,922 + +1.0 1,332,930 - -1.4 1,332,930 - +0.1 1,333,156 + -0.1 1,333,158 - -1.0 1,333,160 - +0.7 1,333,197 + yciX_1 b4522 0.10 +0.0 1,333,198 + yciX_1 b4522 0.11 +0.3 1,333,275 - yciX_1 b4522 0.71 -1.1 1,333,286 - yciX_1 b4522 0.79 +0.0 1,333,286 - yciX_1 b4522 0.79 -1.7 1,333,301 + +0.7 1,333,301 + -1.0 1,333,311 + +0.7 1,333,320 - +1.0 1,333,320 - +1.0 1,333,322 + -0.8 1,333,322 + -0.3 1,333,345 + yciX b4523 0.18 -0.7 1,333,345 + yciX b4523 0.18 -0.1 1,333,345 + yciX b4523 0.18 -0.1 1,333,349 + yciX b4523 0.20 +0.7 1,333,418 + yciX b4523 0.61 -0.1 1,333,420 - yciX b4523 0.62 +1.0 1,333,434 - yciX b4523 0.71 +1.0 1,333,450 - yciX b4523 0.80 +1.0 1,333,450 - yciX b4523 0.80 +0.6 1,333,525 + -0.1 1,333,538 + -0.1 1,333,578 + +0.1 1,333,606 - -1.3 1,333,606 - +0.2 1,333,606 - +0.1 1,333,606 - +1.3 1,333,630 - -0.5 1,333,655 + +0.1 1,333,670 + +0.7 1,333,670 + -0.5 1,333,678 - -1.6 1,333,794 + +0.5 1,333,794 + +0.4 1,333,818 + +0.8 1,333,838 + +0.3 1,333,841 - +0.6 1,333,847 - +0.8 1,333,873 - +0.2 1,333,932 - -0.2 1,334,080 - +0.5 1,334,080 - +0.7 1,334,663 - acnA b1276 0.30 +1.9 1,334,824 - acnA b1276 0.36 -0.0 1,334,824 - acnA b1276 0.36 -0.6 1,334,962 + acnA b1276 0.41 +1.0 1,335,010 + acnA b1276 0.43 +0.6 1,335,010 + acnA b1276 0.43 -1.0 1,335,167 + acnA b1276 0.49 +0.0 1,335,181 + acnA b1276 0.50 +0.6 1,335,573 - acnA b1276 0.64 +0.1 1,335,601 - acnA b1276 0.65 +0.5 1,335,601 - acnA b1276 0.65 -3.1 1,335,669 + acnA b1276 0.68 -0.1 1,335,669 + acnA b1276 0.68 -0.8 1,335,825 - acnA b1276 0.74 -1.2 1,335,825 - acnA b1276 0.74 -0.9 1,335,902 - acnA b1276 0.76 -0.6 1,335,902 - acnA b1276 0.76 +1.2 1,336,129 - acnA b1276 0.85 +0.1 1,336,223 + acnA b1276 0.88 +0.5 1,337,219 - -1.2 1,337,219 - +0.1 1,337,498 + pgpB b1278 0.19 -1.4 1,337,503 + pgpB b1278 0.19 -0.5 1,337,522 - pgpB b1278 0.22 -3.2
Or see this region's nucleotide sequence