Experiment: ppI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt surA and imp are separated by 52 nucleotides imp and djlA are separated by 254 nucleotides
b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702
surA
b0054: imp - organic solvent tolerance protein precursor (NCBI), at 54,755 to 57,109
imp
b0055: djlA - Dna-J like membrane chaperone protein (NCBI), at 57,364 to 58,179
djlA
Position (kb) 54
55
56
57
58 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 53.766 kb on - strand, within surA at 53.766 kb on - strand, within surA at 53.776 kb on - strand, within surA at 53.797 kb on - strand, within surA at 53.802 kb on + strand, within surA at 53.807 kb on - strand, within surA at 53.910 kb on + strand, within surA at 53.991 kb on + strand, within surA at 53.997 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.035 kb on - strand, within surA at 54.048 kb on + strand, within surA at 54.148 kb on + strand, within surA at 54.166 kb on - strand, within surA at 54.166 kb on - strand, within surA at 54.178 kb on + strand, within surA at 54.196 kb on - strand, within surA at 54.205 kb on + strand, within surA at 54.285 kb on - strand, within surA at 54.285 kb on - strand, within surA at 54.406 kb on + strand, within surA at 54.487 kb on - strand, within surA at 54.495 kb on - strand, within surA at 54.507 kb on + strand, within surA at 54.568 kb on - strand, within surA at 54.612 kb on - strand at 54.612 kb on - strand at 54.612 kb on - strand at 54.637 kb on - strand at 54.826 kb on - strand at 57.158 kb on + strand at 57.192 kb on + strand at 57.192 kb on + strand at 57.199 kb on - strand at 57.226 kb on - strand at 57.226 kb on - strand at 57.231 kb on - strand at 57.231 kb on - strand at 57.253 kb on - strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.274 kb on + strand at 57.282 kb on - strand at 57.282 kb on - strand at 57.359 kb on - strand at 57.359 kb on - strand at 57.367 kb on - strand at 57.367 kb on - strand at 57.383 kb on + strand at 57.394 kb on + strand at 57.394 kb on + strand at 57.402 kb on - strand at 57.402 kb on - strand at 57.406 kb on + strand at 57.413 kb on - strand at 57.414 kb on - strand at 57.414 kb on - strand at 57.420 kb on + strand at 57.433 kb on + strand at 57.441 kb on - strand at 57.463 kb on + strand, within djlA at 57.469 kb on - strand, within djlA at 57.479 kb on + strand, within djlA at 57.541 kb on + strand, within djlA at 57.544 kb on - strand, within djlA at 57.585 kb on + strand, within djlA at 57.620 kb on + strand, within djlA at 57.647 kb on - strand, within djlA at 57.647 kb on - strand, within djlA at 57.694 kb on + strand, within djlA at 57.694 kb on + strand, within djlA at 57.787 kb on + strand, within djlA at 57.794 kb on + strand, within djlA at 57.820 kb on + strand, within djlA at 57.823 kb on + strand, within djlA at 57.896 kb on + strand, within djlA at 57.896 kb on + strand, within djlA at 57.906 kb on - strand, within djlA at 57.935 kb on - strand, within djlA at 57.952 kb on + strand, within djlA at 57.952 kb on + strand, within djlA at 57.967 kb on + strand, within djlA at 57.983 kb on + strand, within djlA at 58.034 kb on - strand, within djlA at 58.051 kb on + strand, within djlA at 58.051 kb on + strand, within djlA at 58.067 kb on - strand, within djlA
Per-strain Table
Position Strand Gene LocusTag Fraction ppI remove 53,766 - surA b0053 0.27 -3.0 53,766 - surA b0053 0.27 -1.9 53,776 - surA b0053 0.28 -2.3 53,797 - surA b0053 0.30 -2.5 53,802 + surA b0053 0.30 -2.8 53,807 - surA b0053 0.30 -1.3 53,910 + surA b0053 0.38 -2.6 53,991 + surA b0053 0.45 -2.1 53,997 - surA b0053 0.45 -2.3 54,015 - surA b0053 0.47 -2.5 54,015 - surA b0053 0.47 -2.0 54,015 - surA b0053 0.47 -2.4 54,035 - surA b0053 0.48 -2.5 54,048 + surA b0053 0.49 -1.3 54,148 + surA b0053 0.57 -3.6 54,166 - surA b0053 0.58 -2.0 54,166 - surA b0053 0.58 -1.9 54,178 + surA b0053 0.59 -3.0 54,196 - surA b0053 0.61 -3.4 54,205 + surA b0053 0.61 -2.1 54,285 - surA b0053 0.68 -1.4 54,285 - surA b0053 0.68 -2.0 54,406 + surA b0053 0.77 -1.9 54,487 - surA b0053 0.83 -4.0 54,495 - surA b0053 0.84 -1.4 54,507 + surA b0053 0.85 -1.9 54,568 - surA b0053 0.90 -2.5 54,612 - -2.6 54,612 - -3.4 54,612 - -2.3 54,637 - -1.8 54,826 - +1.0 57,158 + -2.3 57,192 + -0.5 57,192 + +0.2 57,199 - -1.6 57,226 - -0.3 57,226 - -0.5 57,231 - +0.5 57,231 - -0.7 57,253 - -1.2 57,273 + -0.3 57,273 + -1.5 57,274 + +0.1 57,282 - +0.7 57,282 - +0.1 57,359 - +0.2 57,359 - -0.3 57,367 - -0.5 57,367 - +0.6 57,383 + -0.2 57,394 + +1.0 57,394 + -0.7 57,402 - -0.2 57,402 - +0.1 57,406 + +0.6 57,413 - -2.6 57,414 - -0.3 57,414 - -1.4 57,420 + -0.6 57,433 + +0.6 57,441 - -0.0 57,463 + djlA b0055 0.12 +0.3 57,469 - djlA b0055 0.13 -0.3 57,479 + djlA b0055 0.14 -0.0 57,541 + djlA b0055 0.22 -2.5 57,544 - djlA b0055 0.22 +1.1 57,585 + djlA b0055 0.27 -0.6 57,620 + djlA b0055 0.31 +0.9 57,647 - djlA b0055 0.35 -0.8 57,647 - djlA b0055 0.35 -0.5 57,694 + djlA b0055 0.40 -1.1 57,694 + djlA b0055 0.40 +0.8 57,787 + djlA b0055 0.52 -0.5 57,794 + djlA b0055 0.53 -2.6 57,820 + djlA b0055 0.56 -0.5 57,823 + djlA b0055 0.56 +0.5 57,896 + djlA b0055 0.65 -0.4 57,896 + djlA b0055 0.65 +0.2 57,906 - djlA b0055 0.66 -0.0 57,935 - djlA b0055 0.70 -1.3 57,952 + djlA b0055 0.72 +0.3 57,952 + djlA b0055 0.72 -0.2 57,967 + djlA b0055 0.74 +0.3 57,983 + djlA b0055 0.76 -1.1 58,034 - djlA b0055 0.82 +0.2 58,051 + djlA b0055 0.84 -0.1 58,051 + djlA b0055 0.84 -0.9 58,067 - djlA b0055 0.86 +0.7
Or see this region's nucleotide sequence