Strain Fitness in Escherichia coli BW25113 around b2940

Experiment: SIR2-HerA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspeA and yqgB are separated by 8 nucleotidesyqgB and yqgC are separated by 135 nucleotidesyqgC and metK are separated by 303 nucleotides b2938: speA - arginine decarboxylase (NCBI), at 3,081,957 to 3,083,933 speA b2939: yqgB - predicted protein (RefSeq), at 3,083,942 to 3,084,073 yqgB b2940: yqgC - hypothetical protein (NCBI), at 3,084,209 to 3,084,424 yqgC b2942: metK - S-adenosylmethionine synthetase (NCBI), at 3,084,728 to 3,085,882 metK Position (kb) 3084 3085Strain fitness (log2 ratio) -3 -2 -1 0 1at 3083.287 kb on - strand, within speAat 3083.287 kb on - strand, within speAat 3083.305 kb on + strand, within speAat 3083.305 kb on + strand, within speAat 3083.361 kb on + strand, within speAat 3083.361 kb on + strand, within speAat 3083.365 kb on - strand, within speAat 3083.365 kb on - strand, within speAat 3083.408 kb on + strand, within speAat 3083.416 kb on - strand, within speAat 3083.440 kb on - strand, within speAat 3083.440 kb on - strand, within speAat 3083.452 kb on - strand, within speAat 3083.452 kb on - strand, within speAat 3083.462 kb on - strand, within speAat 3083.575 kb on - strand, within speAat 3083.575 kb on - strand, within speAat 3083.618 kb on - strand, within speAat 3083.677 kb on - strand, within speAat 3083.693 kb on + strand, within speAat 3083.693 kb on + strand, within speAat 3083.761 kb on - strandat 3083.772 kb on - strandat 3083.802 kb on - strandat 3083.823 kb on + strandat 3083.834 kb on - strandat 3083.834 kb on - strandat 3083.872 kb on + strandat 3083.874 kb on + strandat 3083.880 kb on - strandat 3083.880 kb on - strandat 3083.911 kb on + strandat 3083.985 kb on + strand, within yqgBat 3084.042 kb on + strand, within yqgBat 3084.150 kb on - strandat 3084.153 kb on + strandat 3084.166 kb on - strandat 3084.181 kb on - strandat 3084.227 kb on + strandat 3084.242 kb on + strand, within yqgCat 3084.259 kb on - strand, within yqgCat 3084.259 kb on - strand, within yqgCat 3084.259 kb on - strand, within yqgCat 3084.285 kb on - strand, within yqgCat 3084.345 kb on - strand, within yqgCat 3084.471 kb on + strandat 3084.632 kb on + strandat 3084.632 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SIR2-HerA
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3,083,287 - speA b2938 0.67 -0.4
3,083,287 - speA b2938 0.67 -1.5
3,083,305 + speA b2938 0.68 -2.0
3,083,305 + speA b2938 0.68 -0.5
3,083,361 + speA b2938 0.71 -2.6
3,083,361 + speA b2938 0.71 -0.4
3,083,365 - speA b2938 0.71 -0.1
3,083,365 - speA b2938 0.71 -2.5
3,083,408 + speA b2938 0.73 -1.1
3,083,416 - speA b2938 0.74 -1.6
3,083,440 - speA b2938 0.75 -3.3
3,083,440 - speA b2938 0.75 -2.2
3,083,452 - speA b2938 0.76 -3.5
3,083,452 - speA b2938 0.76 -1.6
3,083,462 - speA b2938 0.76 -1.3
3,083,575 - speA b2938 0.82 -1.2
3,083,575 - speA b2938 0.82 -1.4
3,083,618 - speA b2938 0.84 -0.3
3,083,677 - speA b2938 0.87 -0.3
3,083,693 + speA b2938 0.88 -2.1
3,083,693 + speA b2938 0.88 -0.1
3,083,761 - -0.8
3,083,772 - -0.5
3,083,802 - -2.3
3,083,823 + -0.9
3,083,834 - -1.0
3,083,834 - -1.7
3,083,872 + -0.9
3,083,874 + -3.3
3,083,880 - +0.6
3,083,880 - +0.3
3,083,911 + -0.4
3,083,985 + yqgB b2939 0.33 -0.7
3,084,042 + yqgB b2939 0.76 -0.9
3,084,150 - +0.7
3,084,153 + -0.2
3,084,166 - +1.0
3,084,181 - -0.9
3,084,227 + -0.3
3,084,242 + yqgC b2940 0.15 -0.3
3,084,259 - yqgC b2940 0.23 -0.1
3,084,259 - yqgC b2940 0.23 +1.0
3,084,259 - yqgC b2940 0.23 +1.1
3,084,285 - yqgC b2940 0.35 +1.1
3,084,345 - yqgC b2940 0.63 +0.6
3,084,471 + -0.1
3,084,632 + +0.3
3,084,632 + -0.2

Or see this region's nucleotide sequence