Experiment: SIR2-HerA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serS and dmsA are separated by 238 nucleotides dmsA and dmsB are separated by 10 nucleotides dmsB and dmsC are separated by 1 nucleotides
b0893: serS - seryl-tRNA synthetase (NCBI), at 938,651 to 939,943
serS
b0894: dmsA - anaerobic dimethyl sulfoxide reductase subunit A (VIMSS), at 940,182 to 942,626
dmsA
b0895: dmsB - dimethyl sulfoxide reductase, anaerobic, subunit B (NCBI), at 942,637 to 943,254
dmsB
b0896: dmsC - dimethyl sulfoxide reductase, anaerobic, subunit C (NCBI), at 943,256 to 944,119
dmsC
Position (kb) 940
941
942
943 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 940.056 kb on + strand at 940.322 kb on - strand at 940.364 kb on - strand at 940.550 kb on + strand, within dmsA at 940.550 kb on + strand, within dmsA at 940.630 kb on + strand, within dmsA at 940.660 kb on - strand, within dmsA at 940.660 kb on - strand, within dmsA at 940.790 kb on + strand, within dmsA at 940.820 kb on + strand, within dmsA at 940.823 kb on - strand, within dmsA at 940.904 kb on - strand, within dmsA at 941.180 kb on - strand, within dmsA at 941.577 kb on - strand, within dmsA at 941.580 kb on - strand, within dmsA at 941.580 kb on - strand, within dmsA at 941.704 kb on - strand, within dmsA at 941.704 kb on - strand, within dmsA at 941.720 kb on - strand, within dmsA at 941.723 kb on + strand, within dmsA at 941.723 kb on + strand, within dmsA at 941.749 kb on + strand, within dmsA at 941.873 kb on - strand, within dmsA at 941.873 kb on - strand, within dmsA at 941.878 kb on + strand, within dmsA at 941.878 kb on + strand, within dmsA at 941.942 kb on + strand, within dmsA at 941.946 kb on - strand, within dmsA at 941.975 kb on - strand, within dmsA at 942.104 kb on + strand, within dmsA at 942.177 kb on - strand, within dmsA at 942.269 kb on + strand, within dmsA at 942.432 kb on + strand at 942.485 kb on + strand at 942.485 kb on + strand at 942.493 kb on - strand at 942.501 kb on - strand at 942.626 kb on - strand at 942.693 kb on - strand at 942.850 kb on + strand, within dmsB at 942.860 kb on + strand, within dmsB at 942.863 kb on - strand, within dmsB at 942.971 kb on - strand, within dmsB at 943.027 kb on - strand, within dmsB at 943.027 kb on - strand, within dmsB at 943.107 kb on - strand, within dmsB at 943.182 kb on + strand, within dmsB at 943.319 kb on - strand at 943.319 kb on - strand at 943.348 kb on + strand, within dmsC at 943.348 kb on + strand, within dmsC at 943.348 kb on + strand, within dmsC at 943.412 kb on - strand, within dmsC at 943.412 kb on - strand, within dmsC at 943.485 kb on + strand, within dmsC
Per-strain Table
Position Strand Gene LocusTag Fraction SIR2-HerA remove 940,056 + -0.0 940,322 - -0.7 940,364 - -0.6 940,550 + dmsA b0894 0.15 -0.9 940,550 + dmsA b0894 0.15 +0.5 940,630 + dmsA b0894 0.18 +0.1 940,660 - dmsA b0894 0.20 -0.4 940,660 - dmsA b0894 0.20 -0.2 940,790 + dmsA b0894 0.25 -0.0 940,820 + dmsA b0894 0.26 +0.0 940,823 - dmsA b0894 0.26 -2.3 940,904 - dmsA b0894 0.30 -0.2 941,180 - dmsA b0894 0.41 -0.4 941,577 - dmsA b0894 0.57 -0.3 941,580 - dmsA b0894 0.57 -1.0 941,580 - dmsA b0894 0.57 -0.8 941,704 - dmsA b0894 0.62 -0.0 941,704 - dmsA b0894 0.62 -3.3 941,720 - dmsA b0894 0.63 -1.2 941,723 + dmsA b0894 0.63 -0.7 941,723 + dmsA b0894 0.63 -0.4 941,749 + dmsA b0894 0.64 -0.3 941,873 - dmsA b0894 0.69 -1.4 941,873 - dmsA b0894 0.69 +0.2 941,878 + dmsA b0894 0.69 -0.6 941,878 + dmsA b0894 0.69 -0.1 941,942 + dmsA b0894 0.72 -0.1 941,946 - dmsA b0894 0.72 +0.1 941,975 - dmsA b0894 0.73 -0.6 942,104 + dmsA b0894 0.79 -0.8 942,177 - dmsA b0894 0.82 -0.8 942,269 + dmsA b0894 0.85 -1.3 942,432 + -1.4 942,485 + +0.1 942,485 + -1.1 942,493 - -1.4 942,501 - -0.8 942,626 - +0.6 942,693 - -0.5 942,850 + dmsB b0895 0.34 -1.1 942,860 + dmsB b0895 0.36 -0.8 942,863 - dmsB b0895 0.37 +0.2 942,971 - dmsB b0895 0.54 -0.8 943,027 - dmsB b0895 0.63 +0.4 943,027 - dmsB b0895 0.63 +0.7 943,107 - dmsB b0895 0.76 -0.6 943,182 + dmsB b0895 0.88 -0.3 943,319 - +0.7 943,319 - +0.9 943,348 + dmsC b0896 0.11 +0.3 943,348 + dmsC b0896 0.11 -0.9 943,348 + dmsC b0896 0.11 +0.0 943,412 - dmsC b0896 0.18 -1.5 943,412 - dmsC b0896 0.18 +0.3 943,485 + dmsC b0896 0.27 +0.7
Or see this region's nucleotide sequence