Experiment: SIR2-HerA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddpD and ddpC overlap by 4 nucleotides ddpC and ddpB overlap by 4 nucleotides ddpB and ddpA are separated by 1 nucleotides
b1484: ddpD - D-ala-D-ala transporter subunit (NCBI), at 1,556,055 to 1,557,041
ddpD
b1485: ddpC - D-ala-D-ala transporter subunit (NCBI), at 1,557,038 to 1,557,934
ddpC
b1486: ddpB - D-ala-D-ala transporter subunit (NCBI), at 1,557,931 to 1,558,953
ddpB
b1487: ddpA - D-ala-D-a la transporter subunit (NCBI), at 1,558,955 to 1,560,505
ddpA
Position (kb)
1557
1558
1559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1557.386 kb on - strand, within ddpC at 1557.505 kb on + strand, within ddpC at 1557.505 kb on + strand, within ddpC at 1557.567 kb on + strand, within ddpC at 1557.628 kb on + strand, within ddpC at 1557.835 kb on + strand, within ddpC at 1557.835 kb on + strand, within ddpC at 1558.000 kb on - strand at 1558.010 kb on - strand at 1558.182 kb on - strand, within ddpB at 1558.182 kb on - strand, within ddpB at 1558.226 kb on + strand, within ddpB at 1558.573 kb on - strand, within ddpB at 1558.909 kb on + strand at 1558.909 kb on + strand at 1558.940 kb on - strand at 1559.057 kb on - strand at 1559.057 kb on - strand at 1559.426 kb on - strand, within ddpA at 1559.525 kb on - strand, within ddpA at 1559.596 kb on - strand, within ddpA at 1559.703 kb on - strand, within ddpA at 1559.785 kb on - strand, within ddpA at 1559.785 kb on - strand, within ddpA
Per-strain Table
Position Strand Gene LocusTag Fraction SIR2-HerA remove 1,557,386 - ddpC b1485 0.39 +0.8 1,557,505 + ddpC b1485 0.52 +1.9 1,557,505 + ddpC b1485 0.52 +0.9 1,557,567 + ddpC b1485 0.59 -0.7 1,557,628 + ddpC b1485 0.66 -1.5 1,557,835 + ddpC b1485 0.89 +0.4 1,557,835 + ddpC b1485 0.89 -0.1 1,558,000 - +0.2 1,558,010 - +0.7 1,558,182 - ddpB b1486 0.25 -1.6 1,558,182 - ddpB b1486 0.25 -3.7 1,558,226 + ddpB b1486 0.29 -2.9 1,558,573 - ddpB b1486 0.63 -0.3 1,558,909 + -1.1 1,558,909 + +1.1 1,558,940 - -1.7 1,559,057 - +1.2 1,559,057 - +0.3 1,559,426 - ddpA b1487 0.30 +0.4 1,559,525 - ddpA b1487 0.37 -2.6 1,559,596 - ddpA b1487 0.41 +0.4 1,559,703 - ddpA b1487 0.48 +0.7 1,559,785 - ddpA b1487 0.54 +1.4 1,559,785 - ddpA b1487 0.54 +0.5
Or see this region's nucleotide sequence