Experiment: SIR2-HerA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafL and yafM are separated by 175 nucleotides yafM and lfhA are separated by 223 nucleotides
b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461
yafL
b0228: yafM - hypothetical protein (NCBI), at 247,637 to 248,134
yafM
b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070
lfhA
Position (kb) 247
248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 246.742 kb on - strand at 246.821 kb on - strand, within yafL at 246.854 kb on + strand, within yafL at 246.859 kb on + strand, within yafL at 247.129 kb on - strand, within yafL at 247.129 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.288 kb on + strand, within yafL at 247.312 kb on - strand, within yafL at 247.409 kb on - strand at 247.493 kb on + strand at 247.502 kb on - strand at 247.729 kb on + strand, within yafM at 247.786 kb on - strand, within yafM at 247.786 kb on - strand, within yafM at 247.797 kb on - strand, within yafM at 247.824 kb on - strand, within yafM at 247.831 kb on - strand, within yafM at 248.035 kb on + strand, within yafM at 248.067 kb on + strand, within yafM at 248.185 kb on - strand at 248.185 kb on - strand at 248.336 kb on + strand at 248.337 kb on - strand at 248.479 kb on + strand at 248.479 kb on + strand at 248.479 kb on - strand at 248.500 kb on + strand at 248.539 kb on - strand, within lfhA at 248.579 kb on + strand, within lfhA at 248.579 kb on + strand, within lfhA at 248.594 kb on + strand, within lfhA at 248.609 kb on + strand, within lfhA at 248.666 kb on - strand, within lfhA at 248.911 kb on + strand, within lfhA at 248.977 kb on - strand, within lfhA at 249.077 kb on + strand, within lfhA at 249.103 kb on - strand, within lfhA
Per-strain Table
Position Strand Gene LocusTag Fraction SIR2-HerA remove 246,742 - +0.5 246,821 - yafL b0227 0.15 +0.5 246,854 + yafL b0227 0.19 -0.3 246,859 + yafL b0227 0.20 +0.8 247,129 - yafL b0227 0.56 -2.3 247,129 - yafL b0227 0.56 +0.8 247,227 - yafL b0227 0.69 +1.5 247,227 - yafL b0227 0.69 -0.1 247,288 + yafL b0227 0.77 -0.5 247,312 - yafL b0227 0.80 -1.7 247,409 - +0.7 247,493 + -2.6 247,502 - -0.5 247,729 + yafM b0228 0.18 -0.2 247,786 - yafM b0228 0.30 -1.7 247,786 - yafM b0228 0.30 -3.1 247,797 - yafM b0228 0.32 -1.0 247,824 - yafM b0228 0.38 +0.0 247,831 - yafM b0228 0.39 -1.4 248,035 + yafM b0228 0.80 +0.6 248,067 + yafM b0228 0.86 -2.3 248,185 - -0.3 248,185 - -1.1 248,336 + -0.6 248,337 - +0.5 248,479 + +0.7 248,479 + -1.9 248,479 - +0.9 248,500 + -0.2 248,539 - lfhA b0229 0.11 +0.5 248,579 + lfhA b0229 0.13 +0.3 248,579 + lfhA b0229 0.13 +1.0 248,594 + lfhA b0229 0.14 -1.5 248,609 + lfhA b0229 0.15 +0.9 248,666 - lfhA b0229 0.18 +1.5 248,911 + lfhA b0229 0.32 -0.6 248,977 - lfhA b0229 0.36 -1.1 249,077 + lfhA b0229 0.42 -0.5 249,103 - lfhA b0229 0.43 -0.4
Or see this region's nucleotide sequence