Experiment: SIR2-HerA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yifK and argX are separated by 102 nucleotides argX and hisR are separated by 57 nucleotides hisR and leuT are separated by 20 nucleotides leuT and proM are separated by 42 nucleotides proM and aslB are separated by 146 nucleotides
b3795: yifK - predicted transporter (NCBI), at 3,978,910 to 3,980,295
yifK
b3796: argX - tRNA-Arg (NCBI), at 3,980,398 to 3,980,474
argX
b3797: hisR - tRNA-His (NCBI), at 3,980,532 to 3,980,608
hisR
b3798: leuT - tRNA-Leu (NCBI), at 3,980,629 to 3,980,715
leuT
b3799: proM - tRNA-Pro (NCBI), at 3,980,758 to 3,980,834
proM
b3800: aslB - predicted regulator of arylsulfatase activity (NCBI), at 3,980,981 to 3,982,216
aslB
Position (kb)
3980
3981 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3979.553 kb on - strand, within yifK at 3979.599 kb on + strand, within yifK at 3979.696 kb on + strand, within yifK at 3979.696 kb on + strand, within yifK at 3979.717 kb on - strand, within yifK at 3979.780 kb on + strand, within yifK at 3979.780 kb on + strand, within yifK at 3979.780 kb on + strand, within yifK at 3979.795 kb on + strand, within yifK at 3979.801 kb on + strand, within yifK at 3979.806 kb on + strand, within yifK at 3979.821 kb on - strand, within yifK at 3979.823 kb on + strand, within yifK at 3979.838 kb on + strand, within yifK at 3979.838 kb on + strand, within yifK at 3979.846 kb on - strand, within yifK at 3979.846 kb on - strand, within yifK at 3979.846 kb on - strand, within yifK at 3979.905 kb on + strand, within yifK at 3979.905 kb on + strand, within yifK at 3979.928 kb on + strand, within yifK at 3979.928 kb on + strand, within yifK at 3979.944 kb on - strand, within yifK at 3979.944 kb on - strand, within yifK at 3979.980 kb on + strand, within yifK at 3980.003 kb on + strand, within yifK at 3980.017 kb on - strand, within yifK at 3980.017 kb on - strand, within yifK at 3980.022 kb on - strand, within yifK at 3980.022 kb on - strand, within yifK at 3980.033 kb on + strand, within yifK at 3980.041 kb on - strand, within yifK at 3980.051 kb on - strand, within yifK at 3980.899 kb on - strand at 3980.909 kb on + strand at 3980.922 kb on + strand at 3980.922 kb on + strand at 3980.978 kb on + strand at 3980.978 kb on + strand at 3980.979 kb on - strand at 3980.979 kb on - strand at 3981.033 kb on + strand at 3981.126 kb on + strand, within aslB at 3981.190 kb on - strand, within aslB at 3981.204 kb on + strand, within aslB at 3981.239 kb on + strand, within aslB at 3981.318 kb on - strand, within aslB at 3981.365 kb on + strand, within aslB at 3981.365 kb on + strand, within aslB at 3981.410 kb on + strand, within aslB at 3981.414 kb on - strand, within aslB at 3981.427 kb on + strand, within aslB at 3981.427 kb on + strand, within aslB at 3981.485 kb on - strand, within aslB at 3981.490 kb on + strand, within aslB at 3981.513 kb on - strand, within aslB
Per-strain Table
Position Strand Gene LocusTag Fraction SIR2-HerA remove 3,979,553 - yifK b3795 0.46 +0.4 3,979,599 + yifK b3795 0.50 -0.3 3,979,696 + yifK b3795 0.57 +0.1 3,979,696 + yifK b3795 0.57 +1.1 3,979,717 - yifK b3795 0.58 -0.1 3,979,780 + yifK b3795 0.63 -0.2 3,979,780 + yifK b3795 0.63 +0.3 3,979,780 + yifK b3795 0.63 -0.8 3,979,795 + yifK b3795 0.64 -0.2 3,979,801 + yifK b3795 0.64 -1.3 3,979,806 + yifK b3795 0.65 +0.9 3,979,821 - yifK b3795 0.66 -0.1 3,979,823 + yifK b3795 0.66 +0.4 3,979,838 + yifK b3795 0.67 +0.6 3,979,838 + yifK b3795 0.67 +0.8 3,979,846 - yifK b3795 0.68 +1.9 3,979,846 - yifK b3795 0.68 -0.6 3,979,846 - yifK b3795 0.68 +1.4 3,979,905 + yifK b3795 0.72 +0.7 3,979,905 + yifK b3795 0.72 -0.4 3,979,928 + yifK b3795 0.73 +1.5 3,979,928 + yifK b3795 0.73 -0.3 3,979,944 - yifK b3795 0.75 +0.4 3,979,944 - yifK b3795 0.75 +0.2 3,979,980 + yifK b3795 0.77 -2.9 3,980,003 + yifK b3795 0.79 +0.5 3,980,017 - yifK b3795 0.80 -1.1 3,980,017 - yifK b3795 0.80 +0.5 3,980,022 - yifK b3795 0.80 +0.9 3,980,022 - yifK b3795 0.80 -0.9 3,980,033 + yifK b3795 0.81 -0.5 3,980,041 - yifK b3795 0.82 +0.8 3,980,051 - yifK b3795 0.82 +1.6 3,980,899 - +0.8 3,980,909 + -0.7 3,980,922 + -0.1 3,980,922 + +1.0 3,980,978 + -1.4 3,980,978 + +0.7 3,980,979 - +1.2 3,980,979 - -0.8 3,981,033 + -0.2 3,981,126 + aslB b3800 0.12 +0.5 3,981,190 - aslB b3800 0.17 -0.5 3,981,204 + aslB b3800 0.18 +1.0 3,981,239 + aslB b3800 0.21 +0.7 3,981,318 - aslB b3800 0.27 +0.2 3,981,365 + aslB b3800 0.31 +0.7 3,981,365 + aslB b3800 0.31 +0.4 3,981,410 + aslB b3800 0.35 -1.1 3,981,414 - aslB b3800 0.35 -2.7 3,981,427 + aslB b3800 0.36 -1.0 3,981,427 + aslB b3800 0.36 +1.2 3,981,485 - aslB b3800 0.41 +1.4 3,981,490 + aslB b3800 0.41 +0.7 3,981,513 - aslB b3800 0.43 +0.1
Or see this region's nucleotide sequence