Experiment: SIR2-HerA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speA and yqgB are separated by 8 nucleotides yqgB and yqgC are separated by 135 nucleotides yqgC and metK are separated by 303 nucleotides metK and galP are separated by 423 nucleotides
b2938: speA - arginine decarboxylase (NCBI), at 3,081,957 to 3,083,933
speA
b2939: yqgB - predicted protein (RefSeq), at 3,083,942 to 3,084,073
yqgB
b2940: yqgC - hypothetical protein (NCBI), at 3,084,209 to 3,084,424
yqgC
b2942: metK - S-adenosylmethionine synthetase (NCBI), at 3,084,728 to 3,085,882
metK
b2943: galP - galactose:H+ symporter (galP) (from data) , at 3,086,306 to 3,087,700
galP
Position (kb)
3084
3085
3086 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3083.761 kb on - strand at 3083.772 kb on - strand at 3083.802 kb on - strand at 3083.823 kb on + strand at 3083.834 kb on - strand at 3083.834 kb on - strand at 3083.872 kb on + strand at 3083.874 kb on + strand at 3083.880 kb on - strand at 3083.880 kb on - strand at 3083.911 kb on + strand at 3083.985 kb on + strand, within yqgB at 3084.042 kb on + strand, within yqgB at 3084.150 kb on - strand at 3084.153 kb on + strand at 3084.166 kb on - strand at 3084.181 kb on - strand at 3084.227 kb on + strand at 3084.242 kb on + strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.285 kb on - strand, within yqgC at 3084.345 kb on - strand, within yqgC at 3084.471 kb on + strand at 3084.632 kb on + strand at 3084.632 kb on + strand at 3085.886 kb on + strand at 3085.886 kb on + strand at 3085.886 kb on + strand at 3085.896 kb on + strand at 3085.984 kb on - strand at 3085.984 kb on - strand at 3086.143 kb on - strand at 3086.233 kb on - strand at 3086.330 kb on + strand at 3086.330 kb on + strand at 3086.370 kb on - strand at 3086.372 kb on + strand at 3086.373 kb on + strand at 3086.482 kb on - strand, within galP at 3086.482 kb on - strand, within galP at 3086.496 kb on + strand, within galP at 3086.660 kb on - strand, within galP at 3086.660 kb on - strand, within galP at 3086.681 kb on - strand, within galP at 3086.681 kb on - strand, within galP at 3086.692 kb on - strand, within galP at 3086.703 kb on - strand, within galP at 3086.757 kb on + strand, within galP at 3086.780 kb on - strand, within galP at 3086.780 kb on - strand, within galP at 3086.797 kb on + strand, within galP
Per-strain Table
Position Strand Gene LocusTag Fraction SIR2-HerA remove 3,083,761 - -0.3 3,083,772 - -0.2 3,083,802 - -3.4 3,083,823 + -0.3 3,083,834 - -1.0 3,083,834 - -1.6 3,083,872 + -2.4 3,083,874 + -2.1 3,083,880 - +0.9 3,083,880 - +0.6 3,083,911 + -0.8 3,083,985 + yqgB b2939 0.33 -1.6 3,084,042 + yqgB b2939 0.76 +0.6 3,084,150 - +0.6 3,084,153 + -0.3 3,084,166 - +1.0 3,084,181 - -1.2 3,084,227 + -0.5 3,084,242 + yqgC b2940 0.15 -0.7 3,084,259 - yqgC b2940 0.23 -0.1 3,084,259 - yqgC b2940 0.23 +1.7 3,084,259 - yqgC b2940 0.23 +1.2 3,084,285 - yqgC b2940 0.35 +1.4 3,084,345 - yqgC b2940 0.63 +1.3 3,084,471 + +0.2 3,084,632 + +1.7 3,084,632 + -1.1 3,085,886 + -0.5 3,085,886 + -0.3 3,085,886 + -0.2 3,085,896 + +0.3 3,085,984 - +1.3 3,085,984 - -0.6 3,086,143 - -1.1 3,086,233 - -1.5 3,086,330 + +1.0 3,086,330 + -0.8 3,086,370 - -1.2 3,086,372 + -0.9 3,086,373 + +1.6 3,086,482 - galP b2943 0.13 +1.3 3,086,482 - galP b2943 0.13 -0.7 3,086,496 + galP b2943 0.14 -0.8 3,086,660 - galP b2943 0.25 +0.2 3,086,660 - galP b2943 0.25 +1.4 3,086,681 - galP b2943 0.27 -1.7 3,086,681 - galP b2943 0.27 +1.2 3,086,692 - galP b2943 0.28 -1.9 3,086,703 - galP b2943 0.28 -0.1 3,086,757 + galP b2943 0.32 +0.6 3,086,780 - galP b2943 0.34 +1.6 3,086,780 - galP b2943 0.34 +0.1 3,086,797 + galP b2943 0.35 -1.8
Or see this region's nucleotide sequence