Strain Fitness in Escherichia coli BW25113 around b1492

Experiment: DRT_type_3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyddW and gadC are separated by 130 nucleotidesgadC and gadB are separated by 155 nucleotides b1491: yddW - predicted liprotein (NCBI), at 1,565,528 to 1,566,847 yddW b1492: gadC - predicted glutamate:gamma-aminobutyric acid antiporter (NCBI), at 1,566,978 to 1,568,513 gadC b1493: gadB - glutamate decarboxylase B, PLP-dependent (NCBI), at 1,568,669 to 1,570,069 gadB Position (kb) 1566 1567 1568 1569Strain fitness (log2 ratio) -3 -2 -1 0 1at 1566.047 kb on + strand, within yddWat 1566.127 kb on - strand, within yddWat 1566.158 kb on + strand, within yddWat 1566.244 kb on - strand, within yddWat 1566.298 kb on + strand, within yddWat 1566.403 kb on - strand, within yddWat 1566.507 kb on - strand, within yddWat 1566.507 kb on - strand, within yddWat 1566.594 kb on - strand, within yddWat 1566.685 kb on + strand, within yddWat 1566.768 kb on - strandat 1566.778 kb on - strandat 1566.964 kb on + strandat 1566.979 kb on - strandat 1566.998 kb on + strandat 1567.129 kb on + strandat 1567.143 kb on + strand, within gadCat 1567.143 kb on + strand, within gadCat 1567.206 kb on - strand, within gadCat 1567.206 kb on - strand, within gadCat 1567.212 kb on - strand, within gadCat 1567.212 kb on - strand, within gadCat 1567.401 kb on - strand, within gadCat 1567.558 kb on + strand, within gadCat 1567.795 kb on - strand, within gadCat 1567.894 kb on + strand, within gadCat 1567.894 kb on + strand, within gadCat 1567.920 kb on - strand, within gadCat 1567.987 kb on - strand, within gadCat 1568.041 kb on + strand, within gadCat 1568.119 kb on - strand, within gadCat 1568.119 kb on - strand, within gadCat 1568.164 kb on + strand, within gadCat 1568.186 kb on - strand, within gadCat 1568.186 kb on - strand, within gadCat 1568.280 kb on - strand, within gadCat 1568.280 kb on - strand, within gadCat 1568.387 kb on - strandat 1568.551 kb on + strandat 1568.658 kb on - strandat 1568.658 kb on - strandat 1568.690 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction DRT_type_3
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1,566,047 + yddW b1491 0.39 +1.0
1,566,127 - yddW b1491 0.45 -0.1
1,566,158 + yddW b1491 0.48 +0.5
1,566,244 - yddW b1491 0.54 +0.2
1,566,298 + yddW b1491 0.58 +1.2
1,566,403 - yddW b1491 0.66 +1.2
1,566,507 - yddW b1491 0.74 -0.2
1,566,507 - yddW b1491 0.74 +0.7
1,566,594 - yddW b1491 0.81 -3.0
1,566,685 + yddW b1491 0.88 +0.2
1,566,768 - -0.0
1,566,778 - +1.2
1,566,964 + +0.0
1,566,979 - +0.6
1,566,998 + -0.1
1,567,129 + -0.1
1,567,143 + gadC b1492 0.11 +0.4
1,567,143 + gadC b1492 0.11 +1.7
1,567,206 - gadC b1492 0.15 -0.4
1,567,206 - gadC b1492 0.15 +0.2
1,567,212 - gadC b1492 0.15 -2.5
1,567,212 - gadC b1492 0.15 +1.2
1,567,401 - gadC b1492 0.28 -1.5
1,567,558 + gadC b1492 0.38 +0.9
1,567,795 - gadC b1492 0.53 -0.0
1,567,894 + gadC b1492 0.60 -1.4
1,567,894 + gadC b1492 0.60 -0.5
1,567,920 - gadC b1492 0.61 +1.3
1,567,987 - gadC b1492 0.66 -0.2
1,568,041 + gadC b1492 0.69 -1.0
1,568,119 - gadC b1492 0.74 +1.6
1,568,119 - gadC b1492 0.74 +1.1
1,568,164 + gadC b1492 0.77 +0.0
1,568,186 - gadC b1492 0.79 +0.7
1,568,186 - gadC b1492 0.79 +0.2
1,568,280 - gadC b1492 0.85 -0.6
1,568,280 - gadC b1492 0.85 +0.5
1,568,387 - -2.8
1,568,551 + -0.3
1,568,658 - -0.1
1,568,658 - -1.5
1,568,690 - +0.6

Or see this region's nucleotide sequence