Strain Fitness in Escherichia coli BW25113 around b3638

Experiment: retron_Ec_48_control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmutM and rpmG are separated by 97 nucleotidesrpmG and rpmB are separated by 20 nucleotidesrpmB and radC are separated by 216 nucleotidesradC and dfp are separated by 171 nucleotides b3635: mutM - formamidopyrimidine-DNA glycosylase (NCBI), at 3,808,366 to 3,809,175 mutM b3636: rpmG - 50S ribosomal protein L33 (NCBI), at 3,809,273 to 3,809,440 rpmG b3637: rpmB - 50S ribosomal protein L28 (NCBI), at 3,809,461 to 3,809,697 rpmB b3638: radC - DNA repair protein (VIMSS), at 3,809,914 to 3,810,582 radC b3639: dfp - flavoprotein affecting synthesis of DNA and pantothenate metabolism (VIMSS), at 3,810,754 to 3,811,974 dfp Position (kb) 3809 3810 3811Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3808.931 kb on + strand, within mutMat 3808.931 kb on + strand, within mutMat 3808.954 kb on - strand, within mutMat 3808.954 kb on - strand, within mutMat 3808.954 kb on - strand, within mutMat 3808.987 kb on - strand, within mutMat 3809.014 kb on + strand, within mutMat 3809.014 kb on + strand, within mutMat 3809.031 kb on - strand, within mutMat 3809.056 kb on - strand, within mutMat 3809.056 kb on - strand, within mutMat 3809.066 kb on - strand, within mutMat 3809.105 kb on - strandat 3809.112 kb on - strandat 3809.114 kb on + strandat 3809.114 kb on + strandat 3809.130 kb on + strandat 3809.138 kb on - strandat 3809.138 kb on - strandat 3809.143 kb on - strandat 3809.177 kb on + strandat 3809.204 kb on + strandat 3809.204 kb on + strandat 3809.204 kb on + strandat 3809.206 kb on + strandat 3809.214 kb on - strandat 3809.214 kb on - strandat 3809.306 kb on - strand, within rpmGat 3809.306 kb on - strand, within rpmGat 3809.384 kb on - strand, within rpmGat 3809.432 kb on - strandat 3809.790 kb on - strandat 3809.851 kb on - strandat 3809.851 kb on - strandat 3809.852 kb on - strandat 3809.867 kb on + strandat 3810.048 kb on + strand, within radCat 3810.048 kb on + strand, within radCat 3810.053 kb on - strand, within radCat 3810.053 kb on - strand, within radCat 3810.053 kb on - strand, within radCat 3810.062 kb on + strand, within radCat 3810.063 kb on + strand, within radCat 3810.072 kb on + strand, within radCat 3810.080 kb on - strand, within radCat 3810.080 kb on - strand, within radCat 3810.087 kb on - strand, within radCat 3810.087 kb on - strand, within radCat 3810.087 kb on - strand, within radCat 3810.267 kb on + strand, within radCat 3810.267 kb on + strand, within radCat 3810.285 kb on - strand, within radCat 3810.317 kb on + strand, within radCat 3810.338 kb on + strand, within radCat 3810.346 kb on - strand, within radCat 3810.369 kb on + strand, within radCat 3810.462 kb on - strand, within radCat 3810.464 kb on + strand, within radCat 3810.464 kb on + strand, within radCat 3810.470 kb on - strand, within radCat 3810.482 kb on - strand, within radCat 3810.555 kb on + strandat 3810.564 kb on + strandat 3810.619 kb on + strandat 3810.729 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction retron_Ec_48_control
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3,808,931 + mutM b3635 0.70 +1.0
3,808,931 + mutM b3635 0.70 +0.3
3,808,954 - mutM b3635 0.73 +0.0
3,808,954 - mutM b3635 0.73 +0.6
3,808,954 - mutM b3635 0.73 +0.4
3,808,987 - mutM b3635 0.77 +0.1
3,809,014 + mutM b3635 0.80 -0.9
3,809,014 + mutM b3635 0.80 -0.5
3,809,031 - mutM b3635 0.82 -1.0
3,809,056 - mutM b3635 0.85 +0.2
3,809,056 - mutM b3635 0.85 -2.0
3,809,066 - mutM b3635 0.86 +0.7
3,809,105 - -1.4
3,809,112 - -0.3
3,809,114 + -0.3
3,809,114 + -1.4
3,809,130 + -0.8
3,809,138 - -0.5
3,809,138 - -0.1
3,809,143 - +0.4
3,809,177 + +0.9
3,809,204 + -0.5
3,809,204 + -0.5
3,809,204 + -1.1
3,809,206 + -1.7
3,809,214 - +0.9
3,809,214 - -2.7
3,809,306 - rpmG b3636 0.20 -0.8
3,809,306 - rpmG b3636 0.20 -0.4
3,809,384 - rpmG b3636 0.66 -1.9
3,809,432 - -0.9
3,809,790 - +0.5
3,809,851 - +1.1
3,809,851 - +1.6
3,809,852 - -1.1
3,809,867 + -0.1
3,810,048 + radC b3638 0.20 +0.4
3,810,048 + radC b3638 0.20 +1.2
3,810,053 - radC b3638 0.21 -1.5
3,810,053 - radC b3638 0.21 +0.6
3,810,053 - radC b3638 0.21 +1.0
3,810,062 + radC b3638 0.22 +0.6
3,810,063 + radC b3638 0.22 -0.1
3,810,072 + radC b3638 0.24 +0.7
3,810,080 - radC b3638 0.25 +1.3
3,810,080 - radC b3638 0.25 +1.7
3,810,087 - radC b3638 0.26 -0.8
3,810,087 - radC b3638 0.26 +1.2
3,810,087 - radC b3638 0.26 -0.1
3,810,267 + radC b3638 0.53 +1.0
3,810,267 + radC b3638 0.53 +0.4
3,810,285 - radC b3638 0.55 +0.4
3,810,317 + radC b3638 0.60 -0.7
3,810,338 + radC b3638 0.63 +0.6
3,810,346 - radC b3638 0.65 +0.3
3,810,369 + radC b3638 0.68 +1.7
3,810,462 - radC b3638 0.82 +0.6
3,810,464 + radC b3638 0.82 -0.3
3,810,464 + radC b3638 0.82 -0.7
3,810,470 - radC b3638 0.83 +0.4
3,810,482 - radC b3638 0.85 +1.1
3,810,555 + +0.5
3,810,564 + -1.3
3,810,619 + -2.7
3,810,729 - -4.0

Or see this region's nucleotide sequence