Strain Fitness in Escherichia coli BW25113 around b2819

Experiment: retron_Ec_48_control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntargA and recD are separated by 61 nucleotidesrecD and recB overlap by 1 nucleotides b2818: argA - N-acetylglutamate synthase (NCBI), at 2,947,264 to 2,948,595 argA b2819: recD - exonuclease V (RecBCD complex), alpha chain (NCBI), at 2,948,657 to 2,950,483 recD b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025 recB Position (kb) 2948 2949 2950 2951Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 2947.748 kb on + strand, within argAat 2947.766 kb on + strand, within argAat 2947.782 kb on - strand, within argAat 2947.782 kb on - strand, within argAat 2947.783 kb on + strand, within argAat 2948.091 kb on + strand, within argAat 2948.357 kb on + strand, within argAat 2948.415 kb on - strand, within argAat 2948.474 kb on + strandat 2948.480 kb on - strandat 2948.485 kb on + strandat 2948.493 kb on - strandat 2948.557 kb on - strandat 2948.584 kb on + strandat 2948.592 kb on - strandat 2948.592 kb on - strandat 2948.630 kb on - strandat 2948.689 kb on - strandat 2948.698 kb on + strandat 2948.711 kb on - strandat 2948.975 kb on + strand, within recDat 2948.983 kb on - strand, within recDat 2948.985 kb on + strand, within recDat 2948.988 kb on - strand, within recDat 2949.122 kb on + strand, within recDat 2949.124 kb on + strand, within recDat 2949.124 kb on + strand, within recDat 2949.159 kb on + strand, within recDat 2949.159 kb on + strand, within recDat 2949.559 kb on - strand, within recDat 2949.564 kb on - strand, within recDat 2949.648 kb on + strand, within recDat 2949.648 kb on - strand, within recDat 2949.648 kb on - strand, within recDat 2949.658 kb on + strand, within recDat 2949.691 kb on - strand, within recDat 2949.691 kb on - strand, within recDat 2949.911 kb on - strand, within recDat 2950.492 kb on + strandat 2950.492 kb on + strandat 2950.500 kb on - strandat 2951.352 kb on - strand, within recB

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Per-strain Table

Position Strand Gene LocusTag Fraction retron_Ec_48_control
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2,947,748 + argA b2818 0.36 -0.7
2,947,766 + argA b2818 0.38 +0.8
2,947,782 - argA b2818 0.39 -0.2
2,947,782 - argA b2818 0.39 -0.3
2,947,783 + argA b2818 0.39 +0.8
2,948,091 + argA b2818 0.62 -0.5
2,948,357 + argA b2818 0.82 +1.7
2,948,415 - argA b2818 0.86 -4.1
2,948,474 + -0.6
2,948,480 - +0.4
2,948,485 + -0.7
2,948,493 - +0.3
2,948,557 - -4.9
2,948,584 + -0.0
2,948,592 - +0.1
2,948,592 - +0.4
2,948,630 - +0.3
2,948,689 - -0.0
2,948,698 + -7.5
2,948,711 - -7.2
2,948,975 + recD b2819 0.17 -5.5
2,948,983 - recD b2819 0.18 -6.5
2,948,985 + recD b2819 0.18 -4.2
2,948,988 - recD b2819 0.18 -4.5
2,949,122 + recD b2819 0.25 -6.6
2,949,124 + recD b2819 0.26 -5.6
2,949,124 + recD b2819 0.26 -4.8
2,949,159 + recD b2819 0.27 -6.0
2,949,159 + recD b2819 0.27 -5.8
2,949,559 - recD b2819 0.49 -7.2
2,949,564 - recD b2819 0.50 -6.1
2,949,648 + recD b2819 0.54 -4.2
2,949,648 - recD b2819 0.54 -7.6
2,949,648 - recD b2819 0.54 -6.5
2,949,658 + recD b2819 0.55 -5.3
2,949,691 - recD b2819 0.57 -3.7
2,949,691 - recD b2819 0.57 -4.0
2,949,911 - recD b2819 0.69 -4.9
2,950,492 + -5.9
2,950,492 + -3.8
2,950,500 - -1.9
2,951,352 - recB b2820 0.25 +1.5

Or see this region's nucleotide sequence