Experiment: retron_Ec_48_control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspU and dkgB are separated by 162 nucleotides dkgB and yafC overlap by 4 nucleotides yafC and yafD are separated by 240 nucleotides
b0206: aspU - tRNA-Asp (NCBI), at 228,928 to 229,004
aspU
b0207: dkgB - 2,5-diketo-D-gluconate reductase B (NCBI), at 229,167 to 229,970
dkgB
b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881
yafC
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
Position (kb) 229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2 at 229.157 kb on - strand at 229.224 kb on - strand at 229.230 kb on - strand at 229.351 kb on - strand, within dkgB at 229.437 kb on - strand, within dkgB at 229.491 kb on - strand, within dkgB at 229.501 kb on - strand, within dkgB at 229.501 kb on - strand, within dkgB at 229.674 kb on - strand, within dkgB at 229.674 kb on - strand, within dkgB at 229.685 kb on + strand, within dkgB at 229.755 kb on + strand, within dkgB at 229.763 kb on - strand, within dkgB at 229.768 kb on - strand, within dkgB at 229.798 kb on + strand, within dkgB at 229.802 kb on + strand, within dkgB at 229.803 kb on + strand, within dkgB at 229.830 kb on + strand, within dkgB at 229.972 kb on - strand at 229.972 kb on - strand at 230.069 kb on - strand, within yafC at 230.208 kb on - strand, within yafC at 230.223 kb on - strand, within yafC at 230.230 kb on + strand, within yafC at 230.244 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.282 kb on + strand, within yafC at 230.282 kb on + strand, within yafC at 230.323 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.572 kb on + strand, within yafC at 230.600 kb on - strand, within yafC at 230.611 kb on + strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 231.017 kb on - strand at 231.145 kb on + strand at 231.161 kb on - strand at 231.161 kb on - strand at 231.183 kb on - strand at 231.235 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.535 kb on + strand, within yafD at 231.583 kb on - strand, within yafD at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction retron_Ec_48_control remove 229,157 - +0.2 229,224 - +0.8 229,230 - -0.3 229,351 - dkgB b0207 0.23 +0.3 229,437 - dkgB b0207 0.34 -2.2 229,491 - dkgB b0207 0.40 +0.5 229,501 - dkgB b0207 0.42 -1.2 229,501 - dkgB b0207 0.42 -2.1 229,674 - dkgB b0207 0.63 +0.6 229,674 - dkgB b0207 0.63 +0.2 229,685 + dkgB b0207 0.64 -0.0 229,755 + dkgB b0207 0.73 +0.1 229,763 - dkgB b0207 0.74 -1.1 229,768 - dkgB b0207 0.75 -0.5 229,798 + dkgB b0207 0.78 -0.2 229,802 + dkgB b0207 0.79 +0.9 229,803 + dkgB b0207 0.79 -1.3 229,830 + dkgB b0207 0.82 +0.1 229,972 - +0.8 229,972 - +0.5 230,069 - yafC b0208 0.11 -0.3 230,208 - yafC b0208 0.26 -0.5 230,223 - yafC b0208 0.28 -1.0 230,230 + yafC b0208 0.29 +1.0 230,244 - yafC b0208 0.30 -1.1 230,276 - yafC b0208 0.34 +0.4 230,276 - yafC b0208 0.34 -0.7 230,282 + yafC b0208 0.34 +0.0 230,282 + yafC b0208 0.34 -2.1 230,323 - yafC b0208 0.39 +0.8 230,549 - yafC b0208 0.64 -0.2 230,549 - yafC b0208 0.64 -1.7 230,572 + yafC b0208 0.66 -1.3 230,600 - yafC b0208 0.69 -1.5 230,611 + yafC b0208 0.70 -0.1 230,732 - yafC b0208 0.84 +0.5 230,732 - yafC b0208 0.84 +0.5 230,732 - yafC b0208 0.84 -0.1 230,785 - yafC b0208 0.89 -0.9 230,785 - yafC b0208 0.89 -0.1 231,017 - -1.2 231,145 + +0.1 231,161 - -0.2 231,161 - +0.5 231,183 - -0.5 231,235 + yafD b0209 0.14 +1.9 231,446 + yafD b0209 0.40 +0.3 231,446 + yafD b0209 0.40 +0.9 231,535 + yafD b0209 0.52 +1.7 231,583 - yafD b0209 0.58 +0.9 231,601 + -0.7 231,621 + yafD b0209 0.62 +0.6 231,627 - yafD b0209 0.63 +1.5 231,627 - yafD b0209 0.63 +1.7 231,659 + yafD b0209 0.67 +0.2 231,659 + yafD b0209 0.67 +0.3 231,664 - yafD b0209 0.68 +1.7 231,664 - yafD b0209 0.68 +1.2 231,667 - yafD b0209 0.68 +1.1 231,755 + yafD b0209 0.79 -0.8 231,828 + yafD b0209 0.88 -1.7 231,828 + yafD b0209 0.88 +0.9 231,837 + yafD b0209 0.89 +1.1 231,851 + +1.9 231,851 + +0.0
Or see this region's nucleotide sequence