Strain Fitness in Escherichia coli BW25113 around b3179

Experiment: GFP_control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsH and rlmE are separated by 99 nucleotidesrlmE and yhbY are separated by 125 nucleotidesyhbY and greA are separated by 155 nucleotides b3178: ftsH - protease, ATP-dependent zinc-metallo (NCBI), at 3,323,023 to 3,324,957 ftsH b3179: rlmE - 23S rRNA U2552 ribose 2'-O-methyltransferase, SAM-dependent (NCBI), at 3,325,057 to 3,325,686 rlmE b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105 yhbY b3181: greA - transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS), at 3,326,261 to 3,326,737 greA Position (kb) 3325 3326Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 3324.988 kb on - strandat 3324.988 kb on - strandat 3325.020 kb on - strandat 3325.021 kb on - strandat 3325.022 kb on - strandat 3325.025 kb on - strandat 3325.061 kb on + strandat 3325.061 kb on + strandat 3325.064 kb on - strandat 3325.064 kb on - strandat 3325.085 kb on - strandat 3325.085 kb on - strandat 3325.749 kb on - strandat 3325.849 kb on + strand, within yhbYat 3325.849 kb on + strandat 3325.883 kb on - strand, within yhbYat 3325.908 kb on - strand, within yhbYat 3325.913 kb on + strand, within yhbYat 3325.921 kb on - strand, within yhbYat 3325.928 kb on + strand, within yhbYat 3325.932 kb on + strand, within yhbYat 3325.939 kb on + strandat 3325.940 kb on - strand, within yhbYat 3325.940 kb on - strand, within yhbYat 3325.976 kb on - strand, within yhbYat 3326.010 kb on - strand, within yhbYat 3326.015 kb on - strand, within yhbYat 3326.015 kb on - strand, within yhbYat 3326.066 kb on - strand, within yhbYat 3326.187 kb on - strandat 3326.190 kb on - strandat 3326.197 kb on + strandat 3326.267 kb on - strandat 3326.278 kb on - strandat 3326.278 kb on - strandat 3326.323 kb on + strand, within greAat 3326.338 kb on - strand, within greAat 3326.500 kb on - strand, within greAat 3326.500 kb on - strand, within greAat 3326.548 kb on + strand, within greAat 3326.548 kb on + strand, within greAat 3326.552 kb on + strand, within greAat 3326.552 kb on + strand, within greAat 3326.602 kb on - strand, within greAat 3326.653 kb on + strand, within greA

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Per-strain Table

Position Strand Gene LocusTag Fraction GFP_control
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3,324,988 - -1.5
3,324,988 - -5.6
3,325,020 - +0.6
3,325,021 - -1.2
3,325,022 - +1.0
3,325,025 - -1.7
3,325,061 + +1.9
3,325,061 + -0.4
3,325,064 - +0.6
3,325,064 - -0.3
3,325,085 - -1.7
3,325,085 - -1.8
3,325,749 - +0.4
3,325,849 + yhbY b3180 0.13 -2.4
3,325,849 + -0.7
3,325,883 - yhbY b3180 0.24 +1.0
3,325,908 - yhbY b3180 0.33 -5.1
3,325,913 + yhbY b3180 0.34 -2.5
3,325,921 - yhbY b3180 0.37 -0.2
3,325,928 + yhbY b3180 0.39 -2.2
3,325,932 + yhbY b3180 0.41 -0.7
3,325,939 + -0.1
3,325,940 - yhbY b3180 0.44 +0.5
3,325,940 - yhbY b3180 0.44 +0.3
3,325,976 - yhbY b3180 0.56 -2.9
3,326,010 - yhbY b3180 0.67 -2.7
3,326,015 - yhbY b3180 0.69 -0.7
3,326,015 - yhbY b3180 0.69 +0.3
3,326,066 - yhbY b3180 0.86 -0.8
3,326,187 - +1.7
3,326,190 - +2.2
3,326,197 + +0.0
3,326,267 - -0.8
3,326,278 - -0.0
3,326,278 - -1.1
3,326,323 + greA b3181 0.13 -4.6
3,326,338 - greA b3181 0.16 -5.4
3,326,500 - greA b3181 0.50 -2.9
3,326,500 - greA b3181 0.50 -2.4
3,326,548 + greA b3181 0.60 -3.5
3,326,548 + greA b3181 0.60 -4.6
3,326,552 + greA b3181 0.61 -5.8
3,326,552 + greA b3181 0.61 -4.8
3,326,602 - greA b3181 0.71 -2.8
3,326,653 + greA b3181 0.82 -4.8

Or see this region's nucleotide sequence